{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,20]],"date-time":"2026-05-20T05:03:27Z","timestamp":1779253407459,"version":"3.51.4"},"reference-count":40,"publisher":"American Society for Clinical Investigation","issue":"20","license":[{"start":{"date-parts":[[2021,10,22]],"date-time":"2021-10-22T00:00:00Z","timestamp":1634860800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Cancer Institute","award":["3R01CA166593-07S1"],"award-info":[{"award-number":["3R01CA166593-07S1"]}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"crossref","award":["T32AR065969"],"award-info":[{"award-number":["T32AR065969"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"crossref","award":["R01 CA166593"],"award-info":[{"award-number":["R01 CA166593"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2021,10,22]]},"abstract":"<jats:p>\n                    Dry eye disease affects over 16 million adults in the US, and the majority of cases are due to Meibomian gland dysfunction. Unfortunately, the identity of the stem cells involved in Meibomian gland development and homeostasis is not well elucidated. Here, we report that loss of\n                    <jats:italic>Krox20<\/jats:italic>\n                    , a zinc finger transcription factor involved in the development of ectoderm-derived tissues, or deletion of KROX20-expressing epithelial cells disrupted Meibomian gland formation and homeostasis, leading to dry eye disease secondary to Meibomian gland dysfunction. Ablation of\n                    <jats:italic>Krox20<\/jats:italic>\n                    -lineage cells in adult mice also resulted in dry eye disease, implicating\n                    <jats:italic>Krox20<\/jats:italic>\n                    in homeostasis of the mature Meibomian gland. Lineage-tracing and expression analyses revealed a restricted KROX20 expression pattern in the ductal areas of the Meibomian gland, although\n                    <jats:italic>Krox20<\/jats:italic>\n                    -lineage cells generate the full, mature Meibomian gland. This suggests that KROX20 marks a stem\/progenitor cell population that differentiates to generate the entire Meibomian gland. Our\n                    <jats:italic>Krox20<\/jats:italic>\n                    mouse models provide a powerful system that delineated the identity of stem cells required for Meibomian gland development and homeostasis and can be used to investigate the factors underlying these processes. They are also robust models of Meibomian gland dysfunction\u2013related dry eye disease, with a potential for use in preclinical therapeutic screening.\n                  <\/jats:p>","DOI":"10.1172\/jci.insight.151078","type":"journal-article","created":{"date-parts":[[2021,9,9]],"date-time":"2021-09-09T12:04:36Z","timestamp":1631189076000},"source":"Crossref","is-referenced-by-count":25,"title":["Epithelial stem cell homeostasis in Meibomian gland development, dysfunction, and dry eye disease"],"prefix":"10.1172","volume":"6","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2874-6472","authenticated-orcid":true,"given":"Edem","family":"Tchegnon","sequence":"first","affiliation":[{"name":"Department of Dermatology and"},{"name":"Genetics, Development and Disease Graduate Program, University of Texas Southwestern Medical Center, Dallas, Texas, 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