{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T22:03:39Z","timestamp":1790805819531,"version":"4.1.0"},"reference-count":31,"publisher":"American Society for Clinical Investigation","issue":"20","license":[{"start":{"date-parts":[[2022,10,24]],"date-time":"2022-10-24T00:00:00Z","timestamp":1666569600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"the Ministry of Health, Labor, and Welfare of Japan and the Japan Society for the Promotion of Science","award":["Grants-in-Aid for Scientific Research [KAKENHI 16H06279 (PAGS),18K08338,19H05653,221S0002,"],"award-info":[{"award-number":["Grants-in-Aid for Scientific Research [KAKENHI 16H06279 (PAGS),18K08338,19H05653,221S0002,"]}]},{"name":"the Ministry of Health, Labor, and Welfare of Japan and the Japan Society for the Promotion of Science","award":["19H05746]"],"award-info":[{"award-number":["19H05746]"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2022,10,24]]},"abstract":"<jats:p>\n                    POEMS syndrome is a rare monoclonal plasma cell disorder, with unique symptoms distinct from those of other plasma cell neoplasms, including high serum VEGF levels. Because the prospective isolation of POEMS clones has not yet been successful, their real nature remains unclear. Herein, we performed single-cell RNA-Seq of BM plasma cells from patients with POEMS syndrome and identified POEMS clones that had Ig \u03bb light chain (\n                    <jats:italic>IGL<\/jats:italic>\n                    ) sequences (\n                    <jats:italic>IGLV1-36<\/jats:italic>\n                    ,\n                    <jats:italic>-40<\/jats:italic>\n                    ,\n                    <jats:italic>-44<\/jats:italic>\n                    , and -\n                    <jats:italic>47<\/jats:italic>\n                    ) with amino acid changes specific to POEMS syndrome. The proportions of POEMS clones in plasma cells were markedly smaller than in patients with multiple myeloma (MM) and patients with monoclonal gammopathy of undetermined significance (MGUS). Single-cell transcriptomes revealed that POEMS clones were CD19\n                    <jats:sup>+<\/jats:sup>\n                    , CD138\n                    <jats:sup>+<\/jats:sup>\n                    , and MHC class II\n                    <jats:sup>lo<\/jats:sup>\n                    , which allowed for their prospective isolation. POEMS clones expressed significantly lower levels of\n                    <jats:italic>c-<\/jats:italic>\n                    <jats:italic>MYC<\/jats:italic>\n                    and\n                    <jats:italic>CCND1<\/jats:italic>\n                    than MM clones, accounting for their small size.\n                    <jats:italic>VEGF<\/jats:italic>\n                    mRNA was not upregulated in POEMS clones, directly indicating that VEGF is not produced by POEMS clones. These results reveal unique features of POEMS clones and enhance our understanding of the pathogenesis of POEMS syndrome.\n                  <\/jats:p>","DOI":"10.1172\/jci.insight.151482","type":"journal-article","created":{"date-parts":[[2022,9,21]],"date-time":"2022-09-21T12:00:28Z","timestamp":1663761628000},"source":"Crossref","is-referenced-by-count":11,"title":["Unraveling unique features of plasma cell clones in POEMS syndrome with single-cell analysis"],"prefix":"10.1172","volume":"7","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0789-6789","authenticated-orcid":true,"given":"Yusuke","family":"Isshiki","sequence":"first","affiliation":[{"name":"Department of Hematology, Chiba University Hospital, Chiba, Japan."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Motohiko","family":"Oshima","sequence":"additional","affiliation":[{"name":"Division of Stem Cell and Molecular Medicine, Center for Stem Cell Biology and Regenerative Medicine, The Institute of Medical Science, The University of Tokyo, Tokyo, Japan."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Naoya","family":"Mimura","sequence":"additional","affiliation":[{"name":"Department of Hematology, Chiba University Hospital, Chiba, Japan."},{"name":"Department of Transfusion Medicine and Cell Therapy, Chiba University Hospital, Chiba, Japan."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6140-3135","authenticated-orcid":true,"given":"Kensuke","family":"Kayamori","sequence":"additional","affiliation":[{"name":"Department of Hematology, Chiba University Hospital, Chiba, Japan."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yurie","family":"Miyamoto-Nagai","sequence":"additional","affiliation":[{"name":"Department of Hematology, Chiba University Hospital, Chiba, 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