{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,21]],"date-time":"2026-07-21T14:01:35Z","timestamp":1784642495329,"version":"3.55.0"},"reference-count":46,"publisher":"American Society for Clinical Investigation","issue":"4","license":[{"start":{"date-parts":[[2022,2,22]],"date-time":"2022-02-22T00:00:00Z","timestamp":1645488000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000050","name":"NHLBI","doi-asserted-by":"crossref","award":["RO1-HL132115"],"award-info":[{"award-number":["RO1-HL132115"]}],"id":[{"id":"10.13039\/100000050","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100000050","name":"NHLBI","doi-asserted-by":"crossref","award":["RO1-HL150587"],"award-info":[{"award-number":["RO1-HL150587"]}],"id":[{"id":"10.13039\/100000050","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2022,2,22]]},"abstract":"<jats:p>\n                    Lung alveolar type 2 (AT2) cells are progenitors for alveolar type 1 (AT1) cells. Although many factors regulate AT2 cell plasticity, the role of mitochondrial calcium (\n                    <jats:sub>m<\/jats:sub>\n                    Ca\n                    <jats:sup>2+<\/jats:sup>\n                    ) uptake in controlling AT2 cells remains unclear. We previously identified that the miR-302 family supports lung epithelial progenitor cell proliferation and less differentiated phenotypes during development. Here, we report that a sustained elevation of miR-302 in adult AT2 cells decreases AT2-to-AT1 cell differentiation during the\n                    <jats:italic>Streptococcus pneumoniae<\/jats:italic>\n                    \u2013induced lung injury repair. We identified that miR-302 targets and represses the expression of mitochondrial Ca\n                    <jats:sup>2+<\/jats:sup>\n                    uptake 1 (MICU1), which regulates\n                    <jats:sub>m<\/jats:sub>\n                    Ca\n                    <jats:sup>2+<\/jats:sup>\n                    uptake through the\n                    <jats:sub>m<\/jats:sub>\n                    Ca\n                    <jats:sup>2+<\/jats:sup>\n                    uniporter channel by acting as a gatekeeper at low cytosolic Ca\n                    <jats:sup>2+<\/jats:sup>\n                    levels. Our results reveal a marked increase in MICU1 protein expression and decreased\n                    <jats:sub>m<\/jats:sub>\n                    Ca\n                    <jats:sup>2+<\/jats:sup>\n                    uptake during AT2-to-AT1 cell differentiation in the adult lung. Deletion of\n                    <jats:italic>Micu1<\/jats:italic>\n                    in AT2 cells reduces AT2-to-AT1 cell differentiation during steady-state tissue maintenance and alveolar epithelial regeneration after bacterial pneumonia. These studies indicate that\n                    <jats:sub>m<\/jats:sub>\n                    Ca\n                    <jats:sup>2+<\/jats:sup>\n                    uptake is extensively modulated during AT2-to-AT1 cell differentiation and that MICU1-dependent\n                    <jats:sub>m<\/jats:sub>\n                    Ca\n                    <jats:sup>2+<\/jats:sup>\n                    uniporter channel gating is a prominent mechanism modulating AT2-to-AT1 cell differentiation.\n                  <\/jats:p>","DOI":"10.1172\/jci.insight.154447","type":"journal-article","created":{"date-parts":[[2022,1,20]],"date-time":"2022-01-20T12:00:33Z","timestamp":1642680033000},"source":"Crossref","is-referenced-by-count":25,"title":["MICU1-dependent mitochondrial calcium uptake regulates lung alveolar type 2 cell plasticity and lung 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