{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,31]],"date-time":"2026-10-31T05:35:53Z","timestamp":1793424953526,"version":"4.5.0"},"reference-count":47,"publisher":"American Society for Clinical Investigation","issue":"7","license":[{"start":{"date-parts":[[2023,4,10]],"date-time":"2023-04-10T00:00:00Z","timestamp":1681084800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100006920","name":"University of Pennsylvania","doi-asserted-by":"crossref","award":["the Melissa"],"award-info":[{"award-number":["the Melissa"]}],"id":[{"id":"10.13039\/100006920","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100006920","name":"University of Pennsylvania","doi-asserted-by":"crossref","award":["Paul Anderson Gift Fund"],"award-info":[{"award-number":["Paul Anderson Gift Fund"]}],"id":[{"id":"10.13039\/100006920","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2023,4,10]]},"abstract":"<jats:p>\n                    B cells within secondary lymphoid tissues encompass a diversity of activation states and multiple maturation processes that reflect antigen recognition and transition through the germinal center (GC) reaction, in which mature B cells differentiate into memory and antibody-secreting cells (ASCs). Here, utilizing single-cell RNA-seq, we identify a range of distinct activation and maturation states of tonsil-derived B cells. In particular, we identify what we believe is a previously uncharacterized\n                    <jats:italic>CCL4<\/jats:italic>\n                    \/\n                    <jats:italic>CCL3<\/jats:italic>\n                    chemokine\u2013expressing B cell population with an expression pattern consistent with B cell receptor\/CD40 activation. Furthermore, we present a computational method that leverages regulatory network inference and pseudotemporal modeling to identify upstream transcription factor modulation along a GC-to-ASC axis of transcriptional maturation. Our data set provides valuable insight into diverse B cell functional profiles and will be a useful resource for further studies into the B cell immune compartment.\n                  <\/jats:p>","DOI":"10.1172\/jci.insight.155199","type":"journal-article","created":{"date-parts":[[2023,2,21]],"date-time":"2023-02-21T12:00:40Z","timestamp":1676980840000},"source":"Crossref","is-referenced-by-count":29,"title":["Distinct stage-specific transcriptional states of B cells derived from human tonsillar tissue"],"prefix":"10.1172","volume":"8","author":[{"given":"Diego A.","family":"Espinoza","sequence":"first","affiliation":[{"name":"Center for Neuroinflammation and Experimental Therapeutics, and"},{"name":"Department of Neurology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carole","family":"Le Coz","sequence":"additional","affiliation":[{"name":"Division of Immunology and Allergy, Children\u2019s Hospital of Philadelphia, Philadelphia, Pennsylvania, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emylette","family":"Cruz Cabrera","sequence":"additional","affiliation":[{"name":"Division of Immunology and Allergy, Children\u2019s Hospital of Philadelphia, Philadelphia, Pennsylvania, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Neil","family":"Romberg","sequence":"additional","affiliation":[{"name":"Division of Immunology and Allergy, Children\u2019s Hospital of Philadelphia, Philadelphia, Pennsylvania, USA."},{"name":"Department of Pediatrics, Perelman School of Medicine, Philadelphia, Pennsylvania, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7179-0335","authenticated-orcid":true,"given":"Amit","family":"Bar-Or","sequence":"additional","affiliation":[{"name":"Center for Neuroinflammation and Experimental Therapeutics, and"},{"name":"Department of Neurology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rui","family":"Li","sequence":"additional","affiliation":[{"name":"Center for Neuroinflammation and Experimental Therapeutics, and"},{"name":"Department of Neurology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"232","published-online":{"date-parts":[[2023,4,10]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","DOI":"10.1038\/nri3857"},{"key":"B2","doi-asserted-by":"publisher","DOI":"10.1038\/s41590-018-0135-x"},{"key":"B3","doi-asserted-by":"publisher","DOI":"10.1038\/ni735"},{"key":"B4","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2018.02044"},{"key":"B5","doi-asserted-by":"publisher","DOI":"10.1126\/sciimmunol.abe6291"},{"key":"B6","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20020201"},{"key":"B7","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.158.3.1108"},{"key":"B8","doi-asserted-by":"publisher","DOI":"10.1038\/nri3814"},{"key":"B9","doi-asserted-by":"publisher","DOI":"10.1038\/ni.1843"},{"key":"B10","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.180.6.3655"},{"key":"B11","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2018.02839"},{"key":"B12","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.179.7.4550"},{"key":"B13","doi-asserted-by":"publisher","DOI":"10.1126\/science.1141229"},{"key":"B14","doi-asserted-by":"publisher","DOI":"10.1002\/gcc.10186"},{"key":"B15","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2014-03-559690"},{"key":"B16","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20121387"},{"key":"B17","doi-asserted-by":"publisher","DOI":"10.1038\/ni.2418"},{"key":"B18","doi-asserted-by":"publisher","DOI":"10.1038\/ni.2428"},{"key":"B19","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.169.5.2507"},{"key":"B20","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2012-03-415380"},{"key":"B21","doi-asserted-by":"publisher","DOI":"10.1038\/s41590-018-0181-4"},{"key":"B22","doi-asserted-by":"publisher","DOI":"10.1038\/s41590-020-0660-2"},{"key":"B23","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20200483"},{"key":"B24","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.4463"},{"key":"B25","doi-asserted-by":"publisher","DOI":"10.1038\/nri3795"},{"key":"B26","doi-asserted-by":"publisher","DOI":"10.1038\/35085509"},{"key":"B27","doi-asserted-by":"publisher","DOI":"10.1038\/ni1357"},{"key":"B28","doi-asserted-by":"publisher","DOI":"10.1038\/ni.3348"},{"key":"B29","doi-asserted-by":"publisher","DOI":"10.1016\/j.cels.2015.12.004"},{"key":"B30","doi-asserted-by":"publisher","DOI":"10.1186\/s12864-018-4772-0"},{"key":"B31","doi-asserted-by":"publisher","DOI":"10.1038\/nri3804"},{"key":"B32","doi-asserted-by":"publisher","DOI":"10.1247\/csf.07044"},{"key":"B33","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.201004062"},{"key":"B34","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.162.8.4455"},{"key":"B35","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2008-07-170415"},{"key":"B36","doi-asserted-by":"publisher","DOI":"10.1111\/bjh.13659"},{"key":"B37","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0076215"},{"key":"B38","doi-asserted-by":"publisher","DOI":"10.1038\/s41591-020-01145-0"},{"key":"B39","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.4437"},{"key":"B40","doi-asserted-by":"publisher","DOI":"10.1186\/s13059-019-1874-1"},{"key":"B41","doi-asserted-by":"publisher","DOI":"10.1038\/nbt.3519"},{"key":"B42","doi-asserted-by":"publisher","DOI":"10.1186\/s13059-014-0550-8"},{"key":"B43","doi-asserted-by":"crossref","unstructured":"Korsunsky I, et al. Presto scales Wilcoxon and auROC analyses to millions of observations [preprint]. https:\/\/doi.org\/10.1101\/653253 Posted on bioRxiv May 29, 2019","DOI":"10.1101\/653253"},{"key":"B44","doi-asserted-by":"crossref","unstructured":"Espinoza DA. Frozen feather and motif files for distinct stage-specific transcriptional states of B cells derived from human tonsillar tissue. 2023; https:\/\/doi.org\/10.5281\/zenodo.7562143","DOI":"10.1172\/jci.insight.155199"},{"key":"B45","doi-asserted-by":"publisher","DOI":"10.1089\/omi.2011.0118"},{"key":"B46","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2019.05.031"},{"key":"B47","doi-asserted-by":"publisher","DOI":"10.1111\/j.2517-6161.1995.tb02031.x"}],"container-title":["JCI Insight"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/insight.jci.org\/articles\/view\/155199\/files\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,18]],"date-time":"2026-05-18T21:56:30Z","timestamp":1779141390000},"score":1,"resource":{"primary":{"URL":"https:\/\/insight.jci.org\/articles\/view\/155199"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,4,10]]},"references-count":47,"journal-issue":{"issue":"7","published-online":{"date-parts":[[2023,4,10]]},"published-print":{"date-parts":[[2023,4,10]]}},"alternative-id":["10.1172\/jci.insight.155199"],"URL":"https:\/\/doi.org\/10.1172\/jci.insight.155199","relation":{"has-preprint":[{"id-type":"doi","id":"10.1101\/2021.08.17.456535","asserted-by":"object"}]},"ISSN":["2379-3708"],"issn-type":[{"value":"2379-3708","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,4,10]]},"article-number":"e155199"}}