{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,19]],"date-time":"2026-05-19T00:08:54Z","timestamp":1779149334381,"version":"3.51.4"},"reference-count":72,"publisher":"American Society for Clinical Investigation","issue":"8","license":[{"start":{"date-parts":[[2022,4,22]],"date-time":"2022-04-22T00:00:00Z","timestamp":1650585600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Cancer Institute","award":["P01 CA108671"],"award-info":[{"award-number":["P01 CA108671"]}]},{"DOI":"10.13039\/100017655","name":"Incyte Corporation","doi-asserted-by":"crossref","award":["N\/A"],"award-info":[{"award-number":["N\/A"]}],"id":[{"id":"10.13039\/100017655","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2022,4,22]]},"abstract":"<jats:p>\n                    Chronic myeloproliferative neoplasms (MPN) frequently evolve to a blast phase (BP) that is almost uniformly resistant to induction chemotherapy or hypomethylating agents. We explored the functional properties, genomic architecture, and cell of origin of MPN-BP initiating cells (IC) using a serial NSG mouse xenograft transplantation model. Transplantation of peripheral blood mononuclear cells (MNC) from 7 of 18 patients resulted in a high degree of leukemic cell chimerism and recreated clinical characteristics of human MPN-BP. The function of MPN-BP ICs was not dependent on the presence of\n                    <jats:italic>JAK2V617F<\/jats:italic>\n                    , a driver mutation associated with the initial underlying MPN. By contrast, multiple MPN-BP IC subclones coexisted within MPN-BP MNCs characterized by different myeloid malignancy gene mutations and cytogenetic abnormalities. MPN-BP ICs in 4 patients exhibited extensive proliferative and self-renewal capacity, as demonstrated by their ability to recapitulate human MPN-BP in serial recipients. These MPN-BP IC subclones underwent extensive continuous clonal competition within individual xenografts and across multiple generations, and their subclonal dynamics were consistent with functional evolution of MPN-BP IC. Finally, we show that MPN-BP ICs originate from not only phenotypically identified hematopoietic stem cells, but also lymphoid-myeloid progenitor cells, which were each characterized by differences in MPN-BP initiating activity and self-renewal capacity.\n                  <\/jats:p>","DOI":"10.1172\/jci.insight.156534","type":"journal-article","created":{"date-parts":[[2022,3,8]],"date-time":"2022-03-08T12:00:46Z","timestamp":1646740846000},"source":"Crossref","is-referenced-by-count":4,"title":["Characterization of disease-propagating stem cells responsible for myeloproliferative neoplasm\u2013blast phase"],"prefix":"10.1172","volume":"7","author":[{"given":"Xiaoli","family":"Wang","sequence":"first","affiliation":[{"name":"Division of Hematology\/Medical Oncology\/Pathology, Tisch Cancer Institute, Icahn School of Medicine at Mount Sinai (ISMMS), New York, New York, USA."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Raajit 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