{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,27]],"date-time":"2026-10-27T17:13:52Z","timestamp":1793121232588,"version":"4.5.0"},"reference-count":58,"publisher":"American Society for Clinical Investigation","issue":"12","funder":[{"DOI":"10.13039\/100000897","name":"Cystic Fibrosis Foundation","doi-asserted-by":"crossref","award":["810400-0621-00"],"award-info":[{"award-number":["810400-0621-00"]}],"id":[{"id":"10.13039\/100000897","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2022,6,22]]},"abstract":"<jats:p>\n                    Nontuberculous mycobacteria (NTM) are an increasingly common cause of respiratory infection in people with cystic fibrosis (PwCF). Relative to those with no history of NTM infection (CF-NTM\n                    <jats:sup>NEG<\/jats:sup>\n                    ), PwCF and a history of NTM infection (CF-NTM\n                    <jats:sup>POS<\/jats:sup>\n                    ) are more likely to develop severe lung disease and experience complications over the course of treatment. In other mycobacterial infections (e.g., tuberculosis), an overexuberant immune response causes pathology and compromises organ function; however, since the immune profiles of CF-NTM\n                    <jats:sup>POS<\/jats:sup>\n                    and CF-NTM\n                    <jats:sup>NEG<\/jats:sup>\n                    airways are largely unexplored, it is unknown which, if any, immune responses distinguish these cohorts or concentrate in damaged tissues. Here, we evaluated lung lobe\u2013specific immune profiles of 3 cohorts (CF-NTM\n                    <jats:sup>POS<\/jats:sup>\n                    , CF-NTM\n                    <jats:sup>NEG<\/jats:sup>\n                    , and non-CF adults) and found that CF-NTM\n                    <jats:sup>POS<\/jats:sup>\n                    airways are distinguished by a hyperinflammatory cytokine profile. Importantly, the CF-NTM\n                    <jats:sup>POS<\/jats:sup>\n                    airway immune profile was dominated by B cells, classical macrophages, and the cytokines that support their accumulation. These and other immunological differences between cohorts, including the near absence of NK cells and complement pathway members, were enriched in the most damaged lung lobes. The implications of these findings for our understanding of lung disease in PwCF are discussed, as are how they may inform the development of host-directed therapies to improve NTM disease treatment.\n                  <\/jats:p>","DOI":"10.1172\/jci.insight.157865","type":"journal-article","created":{"date-parts":[[2022,5,10]],"date-time":"2022-05-10T12:00:21Z","timestamp":1652184021000},"source":"Crossref","is-referenced-by-count":16,"title":["Tissue-localized immune responses in people with cystic fibrosis and respiratory nontuberculous mycobacteria infection"],"prefix":"10.1172","volume":"7","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6734-6052","authenticated-orcid":true,"suffix":"Jr.","given":"Don","family":"Hayes","sequence":"first","affiliation":[{"name":"Cincinnati Children\u2019s Hospital Medical Center, Department of Pediatrics, University of Cincinnati College of Medicine, Cincinnati, Ohio, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5722-449X","authenticated-orcid":true,"given":"Rajni Kant","family":"Shukla","sequence":"additional","affiliation":[{"name":"Department of Microbial Infection and Immunity, The Ohio State University, Columbus, Ohio, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6867-4683","authenticated-orcid":true,"given":"Yizi","family":"Cheng","sequence":"additional","affiliation":[{"name":"Cincinnati Children\u2019s Hospital Medical Center, Department of Pediatrics, University of Cincinnati College of Medicine, Cincinnati, Ohio, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emrah","family":"Gecili","sequence":"additional","affiliation":[{"name":"Cincinnati Children\u2019s Hospital Medical Center, Department of Pediatrics, University of Cincinnati College of Medicine, Cincinnati, Ohio, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marlena R.","family":"Merling","sequence":"additional","affiliation":[{"name":"Department of Microbial Infection and Immunity, The Ohio State University, Columbus, Ohio, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0705-715X","authenticated-orcid":true,"given":"Rhonda D.","family":"Szczesniak","sequence":"additional","affiliation":[{"name":"Cincinnati Children\u2019s Hospital Medical Center, Department of Pediatrics, University of Cincinnati College of Medicine, Cincinnati, Ohio, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8959-7218","authenticated-orcid":true,"given":"Assem G.","family":"Ziady","sequence":"additional","affiliation":[{"name":"Cincinnati Children\u2019s Hospital Medical Center, Department of Pediatrics, University of Cincinnati College of Medicine, Cincinnati, Ohio, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jason C.","family":"Woods","sequence":"additional","affiliation":[{"name":"Cincinnati Children\u2019s Hospital Medical Center, Department of Pediatrics, University of Cincinnati College of Medicine, Cincinnati, Ohio, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2531-4797","authenticated-orcid":true,"given":"Luanne","family":"Hall-Stoodley","sequence":"additional","affiliation":[{"name":"Department of Microbial Infection and Immunity, The Ohio State University, Columbus, Ohio, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7362-3282","authenticated-orcid":true,"given":"Namal P.M.","family":"Liyanage","sequence":"additional","affiliation":[{"name":"Department of Microbial Infection and Immunity, The Ohio State University, Columbus, Ohio, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Richard T.","family":"Robinson","sequence":"additional","affiliation":[{"name":"Department of Microbial Infection and Immunity, The Ohio State University, Columbus, Ohio, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"232","published-online":{"date-parts":[[2022,6,22]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(20)32542-3"},{"key":"B2","doi-asserted-by":"publisher","DOI":"10.1183\/13993003.00592-2015"},{"key":"B3","doi-asserted-by":"publisher","DOI":"10.1055\/s-0038-1651495"},{"key":"B4","doi-asserted-by":"publisher","DOI":"10.1007\/s40506-016-0092-6"},{"key":"B5","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcf.2015.09.007"},{"key":"B6","doi-asserted-by":"publisher","DOI":"10.1136\/thoraxjnl-2015-207983"},{"issue":"suppl 1","key":"B7","doi-asserted-by":"crossref","first-page":"i1","DOI":"10.1136\/thoraxjnl-2015-207360","article-title":"US Cystic Fibrosis Foundation and European Cystic Fibrosis Society consensus recommendations for the management of non-tuberculous mycobacteria in individuals with cystic fibrosis","volume":"71 Suppl 1","author":"Floto","year":"2016","journal-title":"Thorax"},{"key":"B8","doi-asserted-by":"publisher","DOI":"10.1186\/1465-9921-15-41"},{"key":"B9","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcf.2009.12.001"},{"key":"B10","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01257-09"},{"key":"B11","doi-asserted-by":"publisher","DOI":"10.1016\/j.thorsurg.2018.09.008"},{"key":"B12","doi-asserted-by":"publisher","DOI":"10.1002\/ppul.23646"},{"key":"B13","doi-asserted-by":"publisher","DOI":"10.1378\/chest.06-1906"},{"key":"B14","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijmyco.2016.08.002"},{"key":"B15","doi-asserted-by":"publisher","DOI":"10.1111\/apm.12689"},{"key":"B16","doi-asserted-by":"publisher","DOI":"10.1183\/13993003.01102-2015"},{"key":"B17","doi-asserted-by":"publisher","DOI":"10.1016\/j.healun.2006.04.003"},{"key":"B18","doi-asserted-by":"publisher","DOI":"10.1111\/j.1399-3062.2009.00378.x"},{"key":"B19","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.39.2.816-819.2001"},{"key":"B20","doi-asserted-by":"publisher","DOI":"10.1183\/18106838.0303.291"},{"key":"B21","doi-asserted-by":"publisher","DOI":"10.1016\/j.healun.2006.09.003"},{"key":"B22","doi-asserted-by":"publisher","DOI":"10.1111\/imr.12951"},{"key":"B23","doi-asserted-by":"publisher","DOI":"10.1183\/13993003.02127-2017"},{"key":"B24","doi-asserted-by":"publisher","DOI":"10.1183\/09031936.04.00036704"},{"key":"B25","doi-asserted-by":"publisher","DOI":"10.1164\/ajrccm.152.3.7663788"},{"key":"B26","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2334-8-17"},{"key":"B27","article-title":"Use of inhaled GM-CSF in treatment-refractory NTM infection. An open-label, exploratory clinical trial","volume":"58","author":"Thomson","year":"2021","journal-title":"Eur Respir J"},{"key":"B28","doi-asserted-by":"publisher","DOI":"10.1172\/JCI125669"},{"key":"B29","doi-asserted-by":"publisher","DOI":"10.1016\/j.cyto.2014.01.005"},{"key":"B30","doi-asserted-by":"publisher","DOI":"10.1007\/s10875-016-0233-1"},{"key":"B31","doi-asserted-by":"publisher","DOI":"10.1186\/s12879-020-05553-y"},{"key":"B32","doi-asserted-by":"publisher","DOI":"10.1111\/apm.12492"},{"key":"B33","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2013.02.023"},{"issue":"2","key":"B34","doi-asserted-by":"crossref","DOI":"10.1111\/tid.13229","article-title":"Non-tuberculous mycobacteria in lung transplant recipients: prevalence, risk factors, and impact on survival and chronic lung allograft dysfunction","volume":"22","author":"Friedman","year":"2020","journal-title":"Transpl Infect Dis"},{"key":"B35","doi-asserted-by":"publisher","DOI":"10.1016\/j.transproceed.2019.02.028"},{"key":"B36","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmi.2014.07.001"},{"key":"B37","doi-asserted-by":"publisher","DOI":"10.1016\/j.healun.2011.02.007"},{"key":"B38","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1800076"},{"key":"B39","doi-asserted-by":"publisher","DOI":"10.1080\/15548627.2016.1217370"},{"key":"B40","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0096681"},{"key":"B41","doi-asserted-by":"publisher","DOI":"10.1111\/irv.12670"},{"key":"B42","doi-asserted-by":"crossref","DOI":"10.12688\/f1000research.14184.2","article-title":"The convergent epidemiology of tuberculosis and human cytomegalovirus infection","volume":"7","author":"Cobelens","year":"2018","journal-title":"F1000Res"},{"key":"B43","doi-asserted-by":"publisher","DOI":"10.1038\/s41577-020-0282-9"},{"key":"B44","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1900866"},{"key":"B45","doi-asserted-by":"publisher","DOI":"10.1016\/j.imlet.2021.04.010"},{"key":"B46","doi-asserted-by":"publisher","DOI":"10.1513\/AnnalsATS.201501-029OC"},{"key":"B47","doi-asserted-by":"publisher","DOI":"10.1016\/S2213-2600(14)70028-1"},{"key":"B48","doi-asserted-by":"crossref","DOI":"10.3389\/fimmu.2017.00313","article-title":"The impact of the interferon\/TNF-related apoptosis-inducing ligand signaling axis on disease progression in respiratory viral infection and beyond","volume":"8","author":"Peteranderl","year":"2017","journal-title":"Front Immunol"},{"key":"B49","doi-asserted-by":"publisher","DOI":"10.1165\/rcmb.2010-0224OC"},{"key":"B50","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0098915"},{"key":"B51","doi-asserted-by":"publisher","DOI":"10.1165\/rcmb.2014-0256RC"},{"key":"B52","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0144723"},{"key":"B53","doi-asserted-by":"publisher","DOI":"10.1203\/00006450-198612000-00014"},{"key":"B54","doi-asserted-by":"publisher","DOI":"10.1021\/pr900093j"},{"key":"B55","doi-asserted-by":"publisher","DOI":"10.1165\/rcmb.2014-0250OC"},{"issue":"2","key":"B56","first-page":"185","article-title":"Cystic fibrosis is not caused by a defect in the gene coding for human complement C3","volume":"1","author":"Davies","year":"1983","journal-title":"Mol Biol Med"},{"issue":"6","key":"B57","first-page":"602","article-title":"Third component of complement in cystic fibrosis","volume":"28","author":"Holzhauer","year":"1976","journal-title":"Am J Hum Genet"},{"key":"B58","article-title":"CFTR modulators: the changing face of cystic fibrosis in the era of precision medicine","volume":"10","author":"Lopes-Pacheco","year":"2019","journal-title":"Front Pharmacol"}],"container-title":["JCI Insight"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/insight.jci.org\/articles\/view\/157865\/files\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,18]],"date-time":"2026-05-18T23:32:59Z","timestamp":1779147179000},"score":1,"resource":{"primary":{"URL":"https:\/\/insight.jci.org\/articles\/view\/157865"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,6,22]]},"references-count":58,"journal-issue":{"issue":"12","published-online":{"date-parts":[[2022,6,22]]},"published-print":{"date-parts":[[2022,6,22]]}},"alternative-id":["10.1172\/jci.insight.157865"],"URL":"https:\/\/doi.org\/10.1172\/jci.insight.157865","relation":{},"ISSN":["2379-3708"],"issn-type":[{"value":"2379-3708","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,6,22]]},"article-number":"e157865"}}