{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T05:26:36Z","timestamp":1790832396166,"version":"4.1.0"},"reference-count":68,"publisher":"American Society for Clinical Investigation","issue":"9","license":[{"start":{"date-parts":[[2023,5,8]],"date-time":"2023-05-08T00:00:00Z","timestamp":1683504000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["82130030"],"award-info":[{"award-number":["82130030"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["81920108011"],"award-info":[{"award-number":["81920108011"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["81630024"],"award-info":[{"award-number":["81630024"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["82071026"],"award-info":[{"award-number":["82071026"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["82101199"],"award-info":[{"award-number":["82101199"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Tongji Hospital (HUST) Foundation for Excellent Young Scientist","award":["2020YQ07"],"award-info":[{"award-number":["2020YQ07"]}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"crossref","award":["AI137174"],"award-info":[{"award-number":["AI137174"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"crossref","award":["PO1AI145818"],"award-info":[{"award-number":["PO1AI145818"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Key Research and Development Program of Hubei Province","award":["2021BCA119"],"award-info":[{"award-number":["2021BCA119"]}]},{"name":"National Key Research and Development Program of China","award":["2022YFE0131200"],"award-info":[{"award-number":["2022YFE0131200"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2023,5,8]]},"abstract":"<jats:p>\n                    Although the expression of Mex3 RNA-binding family member B (MEX3B) is upregulated in human nasal epithelial cells (HNECs) predominately in the eosinophilic chronic rhinosinusitis (CRS) with nasal polyps (CRSwNP) subtype, its functions as an RNA binding protein in airway epithelial cells remain unknown. Here, we revealed the role of MEX3B based on different subtypes of CRS and demonstrated that MEX3B decreased the TGF-\u03b2 receptor III (\n                    <jats:italic>TGFBR3<\/jats:italic>\n                    ) mRNA level by binding to its 3\u2032 UTR and reducing its stability in HNECs. TGF-\u03b2R3 was found to be a TGF-\u03b22\u2013specific coreceptor in HNECs. Knocking down or overexpressing MEX3B promoted or inhibited TGF-\u03b22\u2013induced phosphorylation of SMAD2 in HNECs, respectively. TGF-\u03b2R3 and phosphorylated SMAD2 levels were downregulated in CRSwNP compared with controls and CRS without nasal polyps with a more prominent downregulation in the eosinophilic CRSwNP. TGF-\u03b22 promoted collagen production in HNECs. Collagen abundance decreased and edema scores increased in CRSwNP compared with control, again more prominently in the eosinophilic type. Collagen expression in eosinophilic CRSwNP was negatively correlated with MEX3B but positively correlated with TGF-\u03b2R3. These results suggest that MEX3B inhibits tissue fibrosis in eosinophilic CRSwNP by downregulating epithelial cell\n                    <jats:italic>TGFBR3<\/jats:italic>\n                    expression; consequently, MEX3B might be a valuable therapeutic target against eosinophilic CRSwNP.\n                  <\/jats:p>","DOI":"10.1172\/jci.insight.159058","type":"journal-article","created":{"date-parts":[[2023,3,28]],"date-time":"2023-03-28T12:00:42Z","timestamp":1680004842000},"source":"Crossref","is-referenced-by-count":10,"title":["MEX3B inhibits collagen production in eosinophilic nasal polyps by downregulating epithelial cell TGFBR3 mRNA stability"],"prefix":"10.1172","volume":"8","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1310-547X","authenticated-orcid":true,"given":"Jin-Xin","family":"Liu","sequence":"first","affiliation":[{"name":"Department of Otolaryngology-Head and Neck Surgery, Tongji Hospital; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ao-Nan","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology-Head and Neck Surgery, Tongji Hospital; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1975-6898","authenticated-orcid":true,"given":"Qihong","family":"Yu","sequence":"additional","affiliation":[{"name":"Hepatic Surgery Center, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China."},{"name":"Clinical Medical Research Center of Hepatic Surgery at Hubei Province, Wuhan, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9803-4086","authenticated-orcid":true,"given":"Ke-Tai","family":"Shi","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology-Head and Neck Surgery, Tongji Hospital; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi-Bo","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology-Head and Neck Surgery, Tongji Hospital; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cui-Lian","family":"Guo","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology-Head and Neck Surgery, Tongji Hospital; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhe-Zheng","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology-Head and Neck Surgery, Tongji Hospital; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yin","family":"Yao","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology-Head and Neck Surgery, Tongji Hospital; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li","family":"Pan","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology-Head and Neck Surgery, Tongji Hospital; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiang","family":"Lu","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology-Head and Neck Surgery, Tongji Hospital; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2082-4643","authenticated-orcid":true,"given":"Kai","family":"Xu","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology-Head and Neck Surgery, Tongji Hospital; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Heng","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology-Head and Neck Surgery, Tongji Hospital; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9182-4969","authenticated-orcid":true,"given":"Ming","family":"Zeng","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology-Head and Neck Surgery, Tongji Hospital; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chaohong","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology-Head and Neck Surgery, Tongji Hospital; and"},{"name":"Department of Pathogen Biology, School of Basic Medicine, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert P.","family":"Schleimer","sequence":"additional","affiliation":[{"name":"Division of Allergy-Immunology, Department of Medicine; and"},{"name":"Department of Otolaryngology, Northwestern University Feinberg School of Medicine, Chicago, Illinois, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ning","family":"Wu","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology-Head and Neck Surgery, Tongji Hospital; and"},{"name":"Department of Immunology, School of Basic Medicine, Tongji Medical College, and"},{"name":"Cell Architecture Research Center, Huazhong University of Science and Technology, Wuhan, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bo","family":"Liao","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology-Head and Neck Surgery, Tongji Hospital; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4168-6702","authenticated-orcid":true,"given":"Zheng","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology-Head and Neck Surgery, Tongji Hospital; and"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"232","published-online":{"date-parts":[[2023,5,8]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","DOI":"10.1038\/s41577-020-00477-9"},{"key":"B2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2020.04.010"},{"key":"B3","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-018-0449-8"},{"key":"B4","doi-asserted-by":"publisher","DOI":"10.1164\/rccm.201603-0529OC"},{"key":"B5","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2019.07.009"},{"key":"B6","doi-asserted-by":"publisher","DOI":"10.1002\/wrna.1582"},{"key":"B7","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2018.05.001"},{"key":"B8","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2013.020"},{"key":"B9","doi-asserted-by":"publisher","DOI":"10.1038\/nsmb.3301"},{"key":"B10","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2015.02.014"},{"key":"B11","doi-asserted-by":"publisher","DOI":"10.1038\/nsmb.2638"},{"key":"B12","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.CCR-17-2483"},{"key":"B13","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2019.08.158"},{"key":"B14","doi-asserted-by":"publisher","DOI":"10.4193\/Rhin20.600"},{"key":"B15","doi-asserted-by":"publisher","DOI":"10.1111\/all.12577"},{"key":"B16","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaip.2016.04.012"},{"key":"B17","doi-asserted-by":"publisher","DOI":"10.1038\/s41572-020-00218-1"},{"key":"B18","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2009.05.017"},{"key":"B19","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2016.05.041"},{"key":"B20","doi-asserted-by":"publisher","DOI":"10.1186\/1939-4551-7-25"},{"key":"B21","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2015.12.1324"},{"key":"B22","doi-asserted-by":"publisher","DOI":"10.1111\/all.12064"},{"key":"B23","doi-asserted-by":"publisher","DOI":"10.1111\/all.15074"},{"key":"B24","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2019.03.026"},{"key":"B25","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2009.04.013"},{"key":"B26","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2222.2011.03898.x"},{"key":"B27","doi-asserted-by":"publisher","DOI":"10.1007\/s11882-007-0073-4"},{"key":"B28","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(93)90368-Z"},{"key":"B29","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.124.4.557"},{"key":"B30","doi-asserted-by":"publisher","DOI":"10.1038\/nrneph.2016.48"},{"key":"B31","doi-asserted-by":"publisher","DOI":"10.1126\/scisignal.aav5183"},{"key":"B32","doi-asserted-by":"publisher","DOI":"10.1126\/scitranslmed.abe0407"},{"key":"B33","doi-asserted-by":"publisher","DOI":"10.1101\/cshperspect.a022061"},{"key":"B34","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2008.07.008"},{"key":"B35","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms12626"},{"key":"B36","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2019.02.034"},{"key":"B37","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2019.03.024"},{"key":"B38","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20190103"},{"key":"B39","doi-asserted-by":"publisher","DOI":"10.5858\/arpa.2017-0412-RA"},{"key":"B40","doi-asserted-by":"publisher","DOI":"10.1111\/jcmm.14211"},{"key":"B41","doi-asserted-by":"publisher","DOI":"10.1172\/JCI65745"},{"key":"B42","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-4652(199903)178:3<275::AID-JCP1>3.0.CO;2-Q"},{"key":"B43","doi-asserted-by":"publisher","DOI":"10.1002\/adma.201801651"},{"key":"B44","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1400753"},{"key":"B45","doi-asserted-by":"publisher","DOI":"10.1189\/jlb.1107782"},{"key":"B46","doi-asserted-by":"publisher","DOI":"10.1016\/j.tibs.2013.08.004"},{"key":"B47","doi-asserted-by":"publisher","DOI":"10.1111\/all.12963"},{"key":"B48","doi-asserted-by":"publisher","DOI":"10.1183\/13993003.00598-2019"},{"key":"B49","doi-asserted-by":"publisher","DOI":"10.1111\/all.12667"},{"key":"B50","doi-asserted-by":"publisher","DOI":"10.1164\/rccm.200905-0740OC"},{"issue":"11","key":"B51","first-page":"2396","article-title":"Expression analysis of URI\/RMP gene in endometrioid adenocarcinoma by tissue microarray immunohistochemistry","volume":"6","author":"Gu","year":"2013","journal-title":"Int J Clin Exp Pathol"},{"key":"B52","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2017.08.045"},{"key":"B53","doi-asserted-by":"publisher","DOI":"10.1111\/all.14612"},{"key":"B54","doi-asserted-by":"publisher","DOI":"10.1016\/j.freeradbiomed.2016.02.010"},{"key":"B55","doi-asserted-by":"publisher","DOI":"10.1111\/cea.12304"},{"key":"B56","doi-asserted-by":"publisher","DOI":"10.1165\/rcmb.2010-0075OC"},{"key":"B57","doi-asserted-by":"publisher","DOI":"10.1111\/j.1398-9995.2008.01847.x"},{"key":"B58","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2020.12.623"},{"key":"B59","doi-asserted-by":"publisher","DOI":"10.3109\/01902148.2015.1033569"},{"key":"B60","doi-asserted-by":"publisher","DOI":"10.1038\/mtna.2012.36"},{"key":"B61","doi-asserted-by":"publisher","DOI":"10.1152\/ajplung.00122.2021"},{"key":"B62","doi-asserted-by":"publisher","DOI":"10.1261\/rna.1175909"},{"key":"B63","doi-asserted-by":"publisher","DOI":"10.1038\/s41385-018-0048-x"},{"key":"B64","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2020.625630"},{"key":"B65","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2017.12.998"},{"key":"B66","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.1226"},{"key":"B67","doi-asserted-by":"publisher","DOI":"10.1002\/advs.202100209"},{"key":"B68","doi-asserted-by":"publisher","DOI":"10.1172\/JCI94233"}],"container-title":["JCI Insight"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/insight.jci.org\/articles\/view\/159058\/files\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,18]],"date-time":"2026-05-18T21:54:50Z","timestamp":1779141290000},"score":1,"resource":{"primary":{"URL":"https:\/\/insight.jci.org\/articles\/view\/159058"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,5,8]]},"references-count":68,"journal-issue":{"issue":"9","published-online":{"date-parts":[[2023,5,8]]},"published-print":{"date-parts":[[2023,5,8]]}},"alternative-id":["10.1172\/jci.insight.159058"],"URL":"https:\/\/doi.org\/10.1172\/jci.insight.159058","relation":{},"ISSN":["2379-3708"],"issn-type":[{"value":"2379-3708","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,5,8]]},"article-number":"e159058"}}