{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T17:54:10Z","timestamp":1790704450073,"version":"4.1.0"},"reference-count":66,"publisher":"American Society for Clinical Investigation","issue":"21","license":[{"start":{"date-parts":[[2023,5,31]],"date-time":"2023-05-31T00:00:00Z","timestamp":1685491200000},"content-version":"vor","delay-in-days":204,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"ARC","award":["Projet Fondation ARC n\u00b0PJA 20161204687"],"award-info":[{"award-number":["Projet Fondation ARC n\u00b0PJA 20161204687"]}]},{"name":"l\u2019Agence Nationale de la Recherche","award":["ANR-19-CE17-0017"],"award-info":[{"award-number":["ANR-19-CE17-0017"]}]},{"name":"l\u2019Agence Nationale de la Recherche","award":["ANR-19-CE17-0021"],"award-info":[{"award-number":["ANR-19-CE17-0021"]}]},{"name":"Fondation Recherche Medicale","award":["Equipes FRM 2018"],"award-info":[{"award-number":["Equipes FRM 2018"]}]},{"name":"Freiburg Institute for Advanced Studies (FRIAS) and University of Strasbourg Institute for Advanced Study","award":["The first joint program"],"award-info":[{"award-number":["The first joint program"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2022,11,8]]},"abstract":"<jats:p>\n                    Malignant melanoma is a major public health issue displaying frequent resistance to targeted therapy and immunotherapy. A major challenge lies in better understanding how melanoma cells evade immune elimination and how tumor growth and metastasis is facilitated by the tumor microenvironment. Here, we show that expression of the cytokine thymic stromal lymphopoietin (TSLP) by epidermal keratinocytes is induced by cutaneous melanoma in both mice and humans. Using genetically engineered models of melanoma and tumor cell grafting combined with TSLP-KO or overexpression, we defined a crosstalk between melanoma cells, keratinocytes, and immune cells in establishing a tumor-promoting microenvironment. Keratinocyte-derived TSLP is induced by signals derived from melanoma cells and subsequently acts via immune cells to promote melanoma progression and metastasis. Furthermore, we show that TSLP signals through TSLP receptor\u2013expressing (TSLPR-expressing) DCs to play an unrecognized role in promoting GATA3\n                    <jats:sup>+<\/jats:sup>\n                    Tregs expressing a gene signature including ST2, CCR8, ICOS, PD-1, CTLA-4, and OX40 and exhibiting a potent suppressive activity on CD8\n                    <jats:sup>+<\/jats:sup>\n                    T cell proliferation and IFN-\u03b3 production. An analogous population of GATA3-expressing Tregs was also identified in human melanoma tumors. Our study provides insights into the role of TSLP in programming a protumoral immune microenvironment in cutaneous melanoma.\n                  <\/jats:p>","DOI":"10.1172\/jci.insight.161438","type":"journal-article","created":{"date-parts":[[2022,9,15]],"date-time":"2022-09-15T12:00:29Z","timestamp":1663243229000},"source":"Crossref","is-referenced-by-count":23,"title":["Keratinocyte-derived cytokine TSLP promotes growth and metastasis of melanoma by regulating the tumor-associated immune microenvironment"],"prefix":"10.1172","volume":"7","author":[{"given":"Wenjin","family":"Yao","sequence":"first","affiliation":[{"name":"Institut de G\u00e9n\u00e9tique et de Biologie Mol\u00e9culaire et Cellulaire (IGBMC), CNRS UMR 7104, Inserm U 1258, University of Strasbourg, Illkirch, France."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Beatriz","family":"German","sequence":"additional","affiliation":[{"name":"Institut de G\u00e9n\u00e9tique et de Biologie Mol\u00e9culaire et 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