{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,17]],"date-time":"2026-06-17T08:32:54Z","timestamp":1781685174227,"version":"3.54.5"},"reference-count":21,"publisher":"American Society for Clinical Investigation","issue":"17","license":[{"start":{"date-parts":[[2022,9,8]],"date-time":"2022-09-08T00:00:00Z","timestamp":1662595200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000050","name":"NHLBI","doi-asserted-by":"crossref","award":["R35 HL139930"],"award-info":[{"award-number":["R35 HL139930"]}],"id":[{"id":"10.13039\/100000050","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100000060","name":"NIAID","doi-asserted-by":"crossref","award":["R01 AI120033"],"award-info":[{"award-number":["R01 AI120033"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Louisiana Board of Regents Endowed Chairs for Eminent Scholars program","award":["N\/A"],"award-info":[{"award-number":["N\/A"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2022,9,8]]},"abstract":"\n Pneumocystis<\/jats:italic>\n is the most common fungal pulmonary infection in children under the age of 5 years. In children with primary immunodeficiency,\n Pneumocystis<\/jats:italic>\n often presents at 3\u20136 months of age, a time period that coincides with the nadir of maternal IgG and when IgM is the dominant Ig isotype. Because B cells are the dominant antigen-presenting cells for\n Pneumocystis<\/jats:italic>\n , we hypothesized the presence of fungal-specific IgMs in humans and mice and that these IgM specificities would predict T cell antigens. We detected fungal-specific IgMs in human and mouse sera and utilized immunoprecipitation to determine whether any antigens were similar across donors. We then assessed T cell responses to these antigens and found anti-\n Pneumocystis<\/jats:italic>\n IgM in WT mice,\n \n Aicda\n \u2013\/\u2013<\/jats:sup>\n <\/jats:italic>\n mice, and in human cord blood. Immunoprecipitation of\n Pneumocystis murina<\/jats:italic>\n with human cord blood identified shared antigens among these donors. Using class II MHC binding prediction, we designed peptides with these antigens and identified robust peptide-specific lung T cell responses after\n P<\/jats:italic>\n .\n murina<\/jats:italic>\n infection. After mice were immunized with 2 of the antigens, adoptive transfer of vaccine-elicited CD4\n +<\/jats:sup>\n T cells showed effector activity, suggesting that these antigens contain protective\n Pneumocystis<\/jats:italic>\n epitopes. These data support the notion that germline-encoded IgM B cell receptors are critical in antigen presentation and T cell priming in early\n Pneumocystis<\/jats:italic>\n infection.\n <\/jats:p>","DOI":"10.1172\/jci.insight.161450","type":"journal-article","created":{"date-parts":[[2022,8,2]],"date-time":"2022-08-02T12:01:20Z","timestamp":1659441680000},"source":"Crossref","is-referenced-by-count":8,"title":["Germline IgM predicts T cell immunity to Pneumocystis"],"prefix":"10.1172","volume":"7","author":[{"given":"Kristin","family":"Noell","sequence":"first","affiliation":[{"name":"Departments of Pediatrics and Medicine, Center for Translational Research in Infection and Inflammation, and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guixiang","family":"Dai","sequence":"additional","affiliation":[{"name":"Departments of Pediatrics and Medicine, Center for Translational Research in Infection and Inflammation, and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5631-2543","authenticated-orcid":true,"given":"Dora","family":"Pungan","sequence":"additional","affiliation":[{"name":"Departments of Pediatrics and Medicine, Center for Translational Research in Infection and Inflammation, and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna","family":"Ebacher","sequence":"additional","affiliation":[{"name":"Departments of Pediatrics and Medicine, Center for Translational Research in Infection and Inflammation, and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Janet E.","family":"McCombs","sequence":"additional","affiliation":[{"name":"Departments of Pediatrics and Medicine, Center for Translational Research in Infection and Inflammation, and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4082-0543","authenticated-orcid":true,"given":"Samuel J.","family":"Landry","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology, Tulane University School of Medicine, New Orleans, Louisiana, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5151-6304","authenticated-orcid":true,"given":"Jay K.","family":"Kolls","sequence":"additional","affiliation":[{"name":"Departments of Pediatrics and Medicine, Center for Translational Research in Infection and Inflammation, 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Insight"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/insight.jci.org\/articles\/view\/161450\/files\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,18]],"date-time":"2026-05-18T23:30:11Z","timestamp":1779147011000},"score":1,"resource":{"primary":{"URL":"https:\/\/insight.jci.org\/articles\/view\/161450"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,9,8]]},"references-count":21,"journal-issue":{"issue":"17","published-online":{"date-parts":[[2022,9,8]]},"published-print":{"date-parts":[[2022,9,8]]}},"alternative-id":["10.1172\/jci.insight.161450"],"URL":"https:\/\/doi.org\/10.1172\/jci.insight.161450","relation":{},"ISSN":["2379-3708"],"issn-type":[{"value":"2379-3708","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,9,8]]},"article-number":"e161450"}}