{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T18:58:42Z","timestamp":1790017122781,"version":"4.0.1"},"reference-count":50,"publisher":"American Society for Clinical Investigation","issue":"2","license":[{"start":{"date-parts":[[2023,1,24]],"date-time":"2023-01-24T00:00:00Z","timestamp":1674518400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["81771971"],"award-info":[{"award-number":["81771971"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"name":"The Science and Technology Commission of Shanghai Municipality","award":["20ZR1411700"],"award-info":[{"award-number":["20ZR1411700"]}]},{"name":"Shanghai Sailing Program","award":["22YF140660"],"award-info":[{"award-number":["22YF140660"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2023,1,24]]},"abstract":"<jats:p>Ciprofloxacin use may be associated with adverse aortic events. However, the mechanism underlying the effect of ciprofloxacin on the progression of thoracic aortic aneurysm (TAA) is not well understood. Using an in vitro microphysiological model, we treated human aortic smooth muscle cells (HASMCs) derived from patients with bicuspid aortic valve\u2013 or tricuspid aortic valve\u2013associated (BAV- or TAV-associated) TAAs with ciprofloxacin. TAA C57BL\/6 mouse models were utilized to verify the effects of ciprofloxacin exposure. In the microphysiological model, real-time PCR, Western blotting, and RNA sequencing showed that ciprofloxacin exposure was associated with a downregulated contractile phenotype, an upregulated inflammatory reaction, and extracellular matrix (ECM) degradation in the normal HASMCs derived from the nondiseased aorta. Ciprofloxacin induced mitochondrial dysfunction in the HASMCs and further increased apoptosis by activating the ERK1\/2 and P38 mitogen\u2013activated protein kinase pathways. These adverse effects appeared to be more severe in the HASMCs derived from BAV- and TAV-associated TAAs than in the normal HASMCs when the ciprofloxacin concentration exceeded 100 \u03bcg\/mL. In the aortic walls of the TAA-induced mice, ECM degradation and apoptosis were aggravated after ciprofloxacin exposure. Therefore, ciprofloxacin should be used with caution in patients with BAV- or TAV-associated TAAs.<\/jats:p>","DOI":"10.1172\/jci.insight.161729","type":"journal-article","created":{"date-parts":[[2022,12,6]],"date-time":"2022-12-06T12:02:06Z","timestamp":1670328126000},"source":"Crossref","is-referenced-by-count":16,"title":["Ciprofloxacin exacerbates dysfunction of smooth muscle cells in a microphysiological model of thoracic aortic aneurysm"],"prefix":"10.1172","volume":"8","author":[{"given":"Bitao","family":"Xiang","sequence":"first","affiliation":[{"name":"Department of Cardiac Surgery, and"},{"name":"Shanghai Institute of Cardiovascular Diseases, Zhongshan Hospital, Fudan University, Shanghai, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mieradilijiang","family":"Abudupataer","sequence":"additional","affiliation":[{"name":"Department of Cardiac Surgery, and"},{"name":"Shanghai Institute of Cardiovascular Diseases, Zhongshan Hospital, Fudan University, 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