{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,29]],"date-time":"2026-10-29T22:55:43Z","timestamp":1793314543899,"version":"4.5.0"},"reference-count":41,"publisher":"American Society for Clinical Investigation","issue":"8","license":[{"start":{"date-parts":[[2023,4,24]],"date-time":"2023-04-24T00:00:00Z","timestamp":1682294400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["82120108013"],"award-info":[{"award-number":["82120108013"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Program for Guangdong Introducing Innovative and Entrepreneurial Teams","award":["2017ZT07S054"],"award-info":[{"award-number":["2017ZT07S054"]}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["81901653"],"award-info":[{"award-number":["81901653"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2023,4,24]]},"abstract":"<jats:p>\n                    Primary Sj\u00f6gren\u2019s syndrome (pSS) is a systemic autoimmune inflammatory disease mainly defined by T cell\u2013dominated destruction of exocrine glands. Currently, CD8\n                    <jats:sup>+<\/jats:sup>\n                    T cells are thought to be involved in the pathogenesis of pSS. However, the single-cell immune profiling of pSS and molecular signatures of pathogenic CD8\n                    <jats:sup>+<\/jats:sup>\n                    T cells have not been well elucidated. Our multiomics investigation showed that both T cells and B cells, especially CD8\n                    <jats:sup>+<\/jats:sup>\n                    T cells, were undergoing significant clonal expansion in pSS patients. TCR clonality analysis revealed that peripheral blood granzyme K\n                    <jats:sup>+<\/jats:sup>\n                    (GZMK\n                    <jats:sup>+<\/jats:sup>\n                    ) CXCR6\n                    <jats:sup>+<\/jats:sup>\n                    CD8\n                    <jats:sup>+<\/jats:sup>\n                    T cells had higher a proportion of clones shared with CD69\n                    <jats:sup>+<\/jats:sup>\n                    CD103\n                    <jats:sup>\u2013<\/jats:sup>\n                    CD8\n                    <jats:sup>+<\/jats:sup>\n                    tissue-resident memory T (Trm) cells in labial glands in pSS. CD69\n                    <jats:sup>+<\/jats:sup>\n                    CD103\n                    <jats:sup>\u2013<\/jats:sup>\n                    CD8\n                    <jats:sup>+<\/jats:sup>\n                    Trm cells featured by high expression of GZMK were more active and cytotoxic in pSS compared with their CD103\n                    <jats:sup>+<\/jats:sup>\n                    counterparts. Peripheral blood GZMK\n                    <jats:sup>+<\/jats:sup>\n                    CXCR6\n                    <jats:sup>+<\/jats:sup>\n                    CD8\n                    <jats:sup>+<\/jats:sup>\n                    T cells with higher CD122 expression were increased and harbored a gene signature similar to Trm cells in pSS. Consistently, IL-15 was significantly elevated in pSS plasma and showed the capacity to promote differentiation of CD8\n                    <jats:sup>+<\/jats:sup>\n                    T cells into GZMK\n                    <jats:sup>+<\/jats:sup>\n                    CXCR6\n                    <jats:sup>+<\/jats:sup>\n                    CD8\n                    <jats:sup>+<\/jats:sup>\n                    T cells in a STAT5-dependent manner. In summary, we depicted the immune profile of pSS and further conducted comprehensive bioinformatics analysis and in vitro experimental investigations to characterize the pathogenic role and differentiation trajectory of CD8\n                    <jats:sup>+<\/jats:sup>\n                    Trm cells in pSS.\n                  <\/jats:p>","DOI":"10.1172\/jci.insight.167490","type":"journal-article","created":{"date-parts":[[2023,3,7]],"date-time":"2023-03-07T12:00:24Z","timestamp":1678190424000},"source":"Crossref","is-referenced-by-count":97,"title":["Single-cell profiling reveals pathogenic role and differentiation trajectory of granzyme K+CD8+ T cells in primary Sj\u00f6gren\u2019s syndrome"],"prefix":"10.1172","volume":"8","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2578-6532","authenticated-orcid":true,"given":"Ting","family":"Xu","sequence":"first","affiliation":[{"name":"Department of Rheumatology and Immunology, Guangdong Provincial People\u2019s Hospital (Guangdong Academy of Medical Sciences), Southern Medical University, Guangzhou, Guangdong, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hao-Xian","family":"Zhu","sequence":"additional","affiliation":[{"name":"School of Medicine, South China University of Technology, Guangzhou, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xing","family":"You","sequence":"additional","affiliation":[{"name":"School of Medicine, South China University of Technology, Guangzhou, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jin-Fen","family":"Ma","sequence":"additional","affiliation":[{"name":"School of Medicine, South China University of Technology, Guangzhou, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xin","family":"Li","sequence":"additional","affiliation":[{"name":"Medical Research Institute, Guangdong Provincial People\u2019s Hospital (Guangdong Academy of Medical Sciences), Southern Medical University, Guangzhou, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pan-Yue","family":"Luo","sequence":"additional","affiliation":[{"name":"School of Biomedical Sciences and Engineering, South China University of Technology, Guangzhou International Campus, Guangzhou, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yang","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Rheumatology and Immunology, Guangdong Provincial People\u2019s Hospital (Guangdong Academy of Medical Sciences), Southern Medical University, Guangzhou, Guangdong, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhe-Xiong","family":"Lian","sequence":"additional","affiliation":[{"name":"Guangdong Provincial People\u2019s Hospital (Guangdong Academy of Medical Sciences), Southern Medical University, Guangzhou, 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