{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,9]],"date-time":"2026-11-09T23:26:37Z","timestamp":1794266797930,"version":"4.9.0"},"reference-count":108,"publisher":"American Society for Clinical Investigation","issue":"20","license":[{"start":{"date-parts":[[2024,10,22]],"date-time":"2024-10-22T00:00:00Z","timestamp":1729555200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000002","name":"National institues of health","doi-asserted-by":"crossref","award":["R01DK123022,R21NS112974,R01NS116008,R21NS125449,R21MH127485"],"award-info":[{"award-number":["R01DK123022,R21NS112974,R01NS116008,R21NS125449,R21MH127485"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,10,22]]},"abstract":"<jats:p>\n                    Solute carrier family 39, member 8 (SLC39A8), is a transmembrane transporter that mediates the cellular uptake of zinc, iron, and manganese (Mn). Human genetic studies document the involvement of SLC39A8 in Mn homeostasis, brain development, and function. However, the role and pathophysiological mechanisms of SLC39A8 in the central nervous system remain elusive. We generated\n                    <jats:italic>Slc39a8<\/jats:italic>\n                    neuron-specific knockout (\n                    <jats:italic>Slc39a8<\/jats:italic>\n                    -NSKO) mice to study SLC39A8 function in neurons. The\n                    <jats:italic>Slc39a8<\/jats:italic>\n                    -NSKO mice displayed markedly decreased Mn levels in the whole brain and brain regions, especially the cerebellum. Radiotracer studies using\n                    <jats:sup>54<\/jats:sup>\n                    Mn revealed that\n                    <jats:italic>Slc39a8<\/jats:italic>\n                    -NSKO mice had impaired brain uptake of Mn.\n                    <jats:italic>Slc39a8<\/jats:italic>\n                    -NSKO cerebellums exhibited morphological defects and abnormal dendritic arborization of Purkinje cells. Reduced neurogenesis and increased apoptotic cell death occurred in the cerebellar external granular layer of\n                    <jats:italic>Slc39a8<\/jats:italic>\n                    -NSKO mice. Brain Mn deficiency in\n                    <jats:italic>Slc39a8<\/jats:italic>\n                    -NSKO mice was associated with motor dysfunction. Unbiased RNA-Seq analysis revealed downregulation of key pathways relevant to neurodevelopment and synaptic plasticity, including cAMP signaling pathway genes. We further demonstrated that Slc39a8 was required for the optimal transcriptional response to the cAMP-mediated signaling pathway. In summary, our study highlighted the essential roles of SLC39A8 in brain Mn uptake and cerebellum development and functions.\n                  <\/jats:p>","DOI":"10.1172\/jci.insight.168440","type":"journal-article","created":{"date-parts":[[2024,10,25]],"date-time":"2024-10-25T09:49:08Z","timestamp":1729849748000},"source":"Crossref","is-referenced-by-count":13,"title":["Neuronal SLC39A8 deficiency impairs cerebellar development by altering manganese homeostasis"],"prefix":"10.1172","volume":"9","author":[{"given":"Eun-Kyung","family":"Choi","sequence":"first","affiliation":[{"name":"Department of Nutritional Sciences, University of Michigan School of Public Health, Ann Arbor, Michigan, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luisa","family":"Aring","sequence":"additional","affiliation":[{"name":"Department of Nutritional Sciences, University of Michigan School of Public Health, Ann Arbor, Michigan, 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