{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,25]],"date-time":"2026-06-25T03:22:42Z","timestamp":1782357762454,"version":"3.54.5"},"reference-count":66,"publisher":"American Society for Clinical Investigation","issue":"23","license":[{"start":{"date-parts":[[2023,12,8]],"date-time":"2023-12-08T00:00:00Z","timestamp":1701993600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"NIH\/NIAMS","award":["R01AR071398-04S1"],"award-info":[{"award-number":["R01AR071398-04S1"]}]},{"name":"NIH\/NIAMS","award":["R01AR072061"],"award-info":[{"award-number":["R01AR072061"]}]},{"name":"NIH\/NIAMS","award":["K99AR081367"],"award-info":[{"award-number":["K99AR081367"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2023,12,8]]},"abstract":"<jats:sec>\n                    <jats:title>BACKGROUND<\/jats:title>\n                    <jats:p>Although 25-hydroxyvitamin D [25(OH)D] concentrations of 30 ng\/mL or higher are known to reduce injury risk and boost strength, the influence on anterior cruciate ligament reconstruction (ACLR) outcomes remains unexamined. This study aimed to define the vitamin D signaling response to ACLR, assess the relationship between vitamin D status and muscle fiber cross-sectional area (CSA) and bone density outcomes, and discover vitamin D receptor (VDR) targets after ACLR.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>METHODS<\/jats:title>\n                    <jats:p>\n                      Twenty-one young, healthy, physically active participants with recent ACL tears were enrolled (17.8 \u00b1 3.2 years, BMI 26.0 \u00b1 3.5 kg\/m\n                      <jats:sup>2<\/jats:sup>\n                      ). Data were collected through blood samples, vastus lateralis biopsies, dual energy x-ray bone density measurements, and isokinetic dynamometer measures at baseline, 1 week, 4 months, and 6 months after ACLR. The biopsies facilitated CSA, Western blotting, RNA-seq, and VDR ChIP-seq analyses.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>RESULTS<\/jats:title>\n                    <jats:p>\n                      ACLR surgery led to decreased circulating bioactive vitamin D and increased VDR and activating enzyme expression in skeletal muscle 1 week after ACLR. Participants with less than 30 ng\/mL 25(OH)D levels (\n                      <jats:italic>n<\/jats:italic>\n                      = 13) displayed more significant quadriceps fiber CSA loss 1 week and 4 months after ACLR than those with 30 ng\/mL or higher (\n                      <jats:italic>n<\/jats:italic>\n                      = 8;\n                      <jats:italic>P<\/jats:italic>\n                      &lt; 0.01 for post hoc comparisons;\n                      <jats:italic>P<\/jats:italic>\n                      = 0.041 for time \u00d7 vitamin D status interaction). RNA-seq and ChIP-seq data integration revealed genes associated with energy metabolism and skeletal muscle recovery, potentially mediating the impact of vitamin D status on ACLR recovery. No difference in bone mineral density losses between groups was observed.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>CONCLUSION<\/jats:title>\n                    <jats:p>Correcting vitamin D status prior to ACLR may aid in preserving skeletal muscle during recovery.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>FUNDING<\/jats:title>\n                    <jats:p>NIH grants R01AR072061, R01AR071398-04S1, and K99AR081367.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1172\/jci.insight.170518","type":"journal-article","created":{"date-parts":[[2023,10,19]],"date-time":"2023-10-19T13:26:45Z","timestamp":1697722005000},"source":"Crossref","is-referenced-by-count":12,"title":["Vitamin D status associates with skeletal muscle loss after anterior cruciate ligament 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