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Mouth dryness is not only due to reduced saliva volume, but also to alterations in the quality of salivary mucins in patients with Sj\u00f6gren\u2019s disease. Mucins play a leading role in mucosa hydration and protection, where sulfated and sialylated oligosaccharides retain water molecules at the epithelial surface. The correct localization of glycosyltransferases and sulfotransferases within the Golgi apparatus determines adequate O-glycosylation and sulfation of mucins, which depends on specific golgins that tether enzyme-bearing vesicles. Here, we show that a golgin called Giantin was mislocalized in salivary glands from patients with Sj\u00f6gren\u2019s disease and formed protein complexes with Gal3-O-sulfotransferases (Gal3STs), which changed their localization in Giantin-knockout and -knockdown cells. Our results suggest that Giantin could tether Gal3ST-bearing vesicles and that its altered localization could affect Gal3ST activity, explaining the decreased sulfation of MUC5B observed in salivary glands from patients with Sj\u00f6gren\u2019s disease.<\/jats:p>","DOI":"10.1172\/jci.insight.171585","type":"journal-article","created":{"date-parts":[[2024,10,10]],"date-time":"2024-10-10T12:00:34Z","timestamp":1728561634000},"source":"Crossref","is-referenced-by-count":6,"title":["Giantin mediates Golgi localization of Gal3-O-sulfotransferases and affects salivary mucin sulfation in patients with Sj\u00f6gren\u2019s disease"],"prefix":"10.1172","volume":"9","author":[{"given":"Matilde","family":"Nu\u00f1ez","sequence":"first","affiliation":[{"name":"Instituto de Ciencias Biom\u00e9dicas, Facultad de Medicina, Universidad de Chile, Santiago, 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