{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T21:53:22Z","timestamp":1790114002790,"version":"4.0.1"},"reference-count":34,"publisher":"American Society for Clinical Investigation","issue":"22","license":[{"start":{"date-parts":[[2024,11,22]],"date-time":"2024-11-22T00:00:00Z","timestamp":1732233600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000069","name":"NIAMS","doi-asserted-by":"crossref","award":["R01AR072018"],"award-info":[{"award-number":["R01AR072018"]}],"id":[{"id":"10.13039\/100000069","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100000072","name":"NIDCR","doi-asserted-by":"crossref","award":["R01DE031288,R01DE031162"],"award-info":[{"award-number":["R01DE031288,R01DE031162"]}],"id":[{"id":"10.13039\/100000072","id-type":"DOI","asserted-by":"crossref"}]},{"name":"NIH","award":["T32DK060445"],"award-info":[{"award-number":["T32DK060445"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,11,22]]},"abstract":"<jats:p>\n                    Human periosteal skeletal stem cells (P-SSCs) are critical for cortical bone maintenance and repair. However, their in vivo identity, molecular characteristics, and specific markers remain unknown. Here, single-cell sequencing revealed human periosteum contains SSC clusters expressing known SSC markers, podoplanin (\n                    <jats:italic>PDPN<\/jats:italic>\n                    ) and\n                    <jats:italic>PDGFRA<\/jats:italic>\n                    . Notably, human P-SSCs, but not bone marrow SSCs, selectively expressed identified markers low density lipoprotein receptor-related protein 1 (LRP1) and CD13. These LRP1\n                    <jats:sup>+<\/jats:sup>\n                    CD13\n                    <jats:sup>+<\/jats:sup>\n                    human P-SSCs were perivascular cells with high osteochondrogenic but minimal adipogenic potential. Upon transplantation into bone injuries in mice, they preserved self-renewal capability in vivo. Single-cell analysis of mouse periosteum further supported the preferential expression of LRP1 and CD13 in\n                    <jats:italic>Prx1<\/jats:italic>\n                    <jats:sup>+<\/jats:sup>\n                    P-SSCs. When\n                    <jats:italic>Lrp1<\/jats:italic>\n                    was conditionally deleted in\n                    <jats:italic>Prx1<\/jats:italic>\n                    lineage cells, it led to severe bone deformity, short stature, and periosteal defects. By contrast, local treatment with an LRP1 agonist at the injury sites induced early P-SSC proliferation and bone healing. Thus, human and mouse periosteum contains unique osteochondrogenic stem cell subsets, and these P-SSCs express specific markers, LRP1 and CD13, with a regulatory mechanism through LRP1 that enhances P-SSC function and bone repair.\n                  <\/jats:p>","DOI":"10.1172\/jci.insight.173831","type":"journal-article","created":{"date-parts":[[2024,10,15]],"date-time":"2024-10-15T12:27:36Z","timestamp":1728995256000},"source":"Crossref","is-referenced-by-count":11,"title":["Identification of LRP1+CD13+ human periosteal stem cells that require LRP1 for bone repair"],"prefix":"10.1172","volume":"9","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2884-5702","authenticated-orcid":true,"given":"Youngjae","family":"Jeong","sequence":"first","affiliation":[{"name":"Department of Molecular and Human Genetics and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lorenzo","family":"Deveza","sequence":"additional","affiliation":[{"name":"Department of Orthopedic Surgery, Baylor College of Medicine, Houston, Texas, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laura","family":"Ortinau","sequence":"additional","affiliation":[{"name":"Department of Molecular and Human Genetics and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kevin","family":"Lei","sequence":"additional","affiliation":[{"name":"Department of Molecular and Human Genetics and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John R.","family":"Dawson","sequence":"additional","affiliation":[{"name":"Department of Orthopedic Surgery, Baylor College of Medicine, Houston, Texas, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2570-3250","authenticated-orcid":true,"given":"Dongsu","family":"Park","sequence":"additional","affiliation":[{"name":"Department of Molecular and Human Genetics 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