{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,10]],"date-time":"2026-10-10T06:47:27Z","timestamp":1791614847704,"version":"4.3.4"},"reference-count":64,"publisher":"American Society for Clinical Investigation","issue":"9","license":[{"start":{"date-parts":[[2025,5,8]],"date-time":"2025-05-08T00:00:00Z","timestamp":1746662400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000265","name":"UK Medical Research Council","doi-asserted-by":"crossref","award":["MR\/J002909,MR\/R00319X\/1"],"award-info":[{"award-number":["MR\/J002909,MR\/R00319X\/1"]}],"id":[{"id":"10.13039\/501100000265","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100012041","name":"Versus Arthritis","doi-asserted-by":"crossref","award":["21809"],"award-info":[{"award-number":["21809"]}],"id":[{"id":"10.13039\/501100012041","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,5,8]]},"abstract":"<jats:p>\n                    Dupuytren\u2019s disease is a common fibroproliferative disease of the palmar fascia of the hand, with advanced cases treated surgically. Anti-TNF injection has undergone phase 2 trials and may be effective in slowing early-stage disease progression. Here we sought to determine how new synthesis of type I collagen in Dupuytren\u2019s differs from normal palmar fascia samples and to analyze the role of TNF in aberrant collagen synthesis. Model nonfibrotic but fibrous connective tissues were used to analyze active type I collagen protein synthesis in development, aging, and degenerative disease, where it was restricted to early development and ruptured tissue. Dupuytren\u2019s tissue was shown to actively synthesize type I collagen, including abnormal type I collagen homotrimer. TNF-\u03b1 reduced\n                    <jats:italic>COL1A2<\/jats:italic>\n                    gene expression only in the presence of serum in 2D cell culture and had opposing effects on collagen protein production in the presence or absence of serum. TNF-\u03b1 had only limited effects in 3D tendon\u2013like constructs. Anti-TNF did not reduce type I collagen synthesis in 3D tendon\u2013like constructs or prevent type I collagen homotrimer synthesis in Dupuytren\u2019s tissue. Hence, modulation of the TNF-\u03b1 pathway in Dupuytren\u2019s disease is unlikely to prevent the pathological collagen accumulation that is characteristic of fibrosis.\n                  <\/jats:p>","DOI":"10.1172\/jci.insight.175188","type":"journal-article","created":{"date-parts":[[2025,5,7]],"date-time":"2025-05-07T14:00:36Z","timestamp":1746626436000},"source":"Crossref","is-referenced-by-count":7,"title":["Active synthesis of type I collagen homotrimer in Dupuytren\u2019s fibrosis is unaffected by anti\u2013TNF-\u03b1 treatment"],"prefix":"10.1172","volume":"10","author":[{"given":"Kate","family":"Williamson","sequence":"first","affiliation":[{"name":"Department of Musculoskeletal and Ageing Science, Institute of Life Course and Medical Sciences, University of Liverpool, William Henry Duncan Building, Liverpool, United Kingdom."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katie J.","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of 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Foundation Trust, Liverpool, United Kingdom."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rob","family":"Pettitt","sequence":"additional","affiliation":[{"name":"Institute of Infection, Veterinary and Ecological Sciences, Leahurst Campus, University of Liverpool, Neston, United Kingdom."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brendan","family":"Geraghty","sequence":"additional","affiliation":[{"name":"Department of Musculoskeletal and Ageing Science, Institute of Life Course and Medical Sciences, University of Liverpool, William Henry Duncan Building, Liverpool, United Kingdom."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lucy A.","family":"Bosworth","sequence":"additional","affiliation":[{"name":"Department of Eye and Vision Sciences, Institute of Life Course and Medical Sciences, University of Liverpool, William Henry Duncan Building, Liverpool, United 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