{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,5]],"date-time":"2026-11-05T22:24:42Z","timestamp":1793917482855,"version":"4.8.0"},"reference-count":56,"publisher":"American Society for Clinical Investigation","issue":"10","license":[{"start":{"date-parts":[[2025,5,22]],"date-time":"2025-05-22T00:00:00Z","timestamp":1747872000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"the Natural Science Foundation of Guangdong Province","award":["2021A1515011113"],"award-info":[{"award-number":["2021A1515011113"]}]},{"name":"the Guangzhou Science and Technology Program","award":["2023A03J0966"],"award-info":[{"award-number":["2023A03J0966"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,5,22]]},"abstract":"<jats:p>\n                    Stem cells play a pivotal role in the malignant behavior of gastric cancer (GC), complicating its treatment and prognosis. However, the regulatory mechanisms of GC stem cells (GCSCs) remain poorly understood. DAZ-associated protein 1 (DAZAP1), a splicing regulator linked to various malignancies, has an unclear role in GC. This study investigated DAZAP1\u2019s impact on GC stemness and its mechanisms. DAZAP1 promoted tumor progression in GCSCs, as shown by sphere formation assays and stemness marker analysis. Functional enrichment analysis suggested that DAZAP1 enhanced tumor stemness by promoting oxidative phosphorylation (OXPHOS), which was validated through Seahorse assays and measurements of mitochondrial potential. Transmission electron microscopy and immunofluorescence analyses demonstrated that DAZAP1 promoted mitophagy. RNA immunoprecipitation and PCR analysis revealed that DAZAP1 regulated the splicing and expression of the mitophagy-related gene\n                    <jats:italic>ULK1<\/jats:italic>\n                    through nonsense-mediated mRNA decay. Rescue experiments showed that overexpression of ULK1 reversed the suppression of GC stemness and OXPHOS levels induced by DAZAP1 silencing. Our findings indicate that DAZAP1 reduces\n                    <jats:italic>ULK1<\/jats:italic>\n                    decay, thereby activating mitophagy and enhancing OXPHOS to fulfill the metabolic demands of cancer stem cells. These findings highlight the therapeutic potential of DAZAP1 as a target for treating GC.\n                  <\/jats:p>","DOI":"10.1172\/jci.insight.175422","type":"journal-article","created":{"date-parts":[[2025,5,21]],"date-time":"2025-05-21T14:00:37Z","timestamp":1747836037000},"source":"Crossref","is-referenced-by-count":10,"title":["DAZAP1 maintains gastric cancer stemness by inducing mitophagy"],"prefix":"10.1172","volume":"10","author":[{"given":"Peiling","family":"Zhang","sequence":"first","affiliation":[{"name":"Department of Medical Oncology, Guangzhou First People\u2019s Hospital, South China University of Technology, Guangzhou, China."},{"name":"Department of Medical Oncology, Guangzhou First People\u2019s Hospital, Guangzhou Medical University, Guangzhou, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Gastric Surgery, Sun Yat-sen University Cancer Center, Guangzhou, 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