{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,17]],"date-time":"2026-10-17T20:57:07Z","timestamp":1792270627395,"version":"4.4.0"},"reference-count":63,"publisher":"American Society for Clinical Investigation","issue":"17","license":[{"start":{"date-parts":[[2024,9,10]],"date-time":"2024-09-10T00:00:00Z","timestamp":1725926400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000052","name":"NIH Office of the Director","doi-asserted-by":"crossref","award":["R01 AI134952,R01 DC016645,the Chronic Rhinosinusitis Integrative Studies Program 2 (CRISP2) P01"],"award-info":[{"award-number":["R01 AI134952,R01 DC016645,the Chronic Rhinosinusitis Integrative Studies Program 2 (CRISP2) P01"]}],"id":[{"id":"10.13039\/100000052","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,9,10]]},"abstract":"<jats:p>\n                    Elevated numbers of antibody-secreting cells (ASCs) and anti\u2013double-stranded DNA (anti-dsDNA) antibodies are found in nasal polyp (NP) tissue. The presence of anti-dsDNA IgG in tissue prospectively predicts recurrent NP but the characteristics of the source ASCs are unknown. Here, we investigated whether NP B cells expressing the extrafollicular marker EBI2 have increased propensity for autoantibody production and evaluated the molecular characteristics of NP ASCs. NPs showed increased frequencies of anti-dsDNA IgG and total IgG ASCs compared with tonsils, with more pronounced differences among EBI2\n                    <jats:sup>+<\/jats:sup>\n                    cells. In NPs, EBI2\n                    <jats:sup>+<\/jats:sup>\n                    cells were frequently double negative (IgD\n                    <jats:sup>\u2013<\/jats:sup>\n                    CD27\n                    <jats:sup>\u2013<\/jats:sup>\n                    ) and ASCs. Single-cell RNA-Seq analysis of tonsils and NPs revealed substantial differences in B lineage composition, including differences in percentages of ASCs, germinal centers, proliferative cells, and non-ASCs. NPs exhibited higher expression of specific isotypes (\n                    <jats:italic>IGHE<\/jats:italic>\n                    ,\n                    <jats:italic>IGHA1<\/jats:italic>\n                    ,\n                    <jats:italic>IGHA2<\/jats:italic>\n                    , and\n                    <jats:italic>IGHG4<\/jats:italic>\n                    ) and mature plasma genes, including\n                    <jats:italic>SDC1<\/jats:italic>\n                    and\n                    <jats:italic>XBP1<\/jats:italic>\n                    , than tonsils. Gene Ontology biological processes indicated upregulated NF-\u03baB and downregulated apoptosis pathways in NP ASCs. Together, these data indicate that NP EBI2\n                    <jats:sup>+<\/jats:sup>\n                    ASCs secret increased total and anti-dsDNA IgG compared with those from tonsils and had molecular features of mature plasma cell differentiation.\n                  <\/jats:p>","DOI":"10.1172\/jci.insight.177729","type":"journal-article","created":{"date-parts":[[2024,9,11]],"date-time":"2024-09-11T15:05:51Z","timestamp":1726067151000},"source":"Crossref","is-referenced-by-count":5,"title":["Increased autoreactivity and maturity of EBI2+ antibody-secreting cells from nasal polyps"],"prefix":"10.1172","volume":"9","author":[{"given":"Junqin","family":"Bai","sequence":"first","affiliation":[{"name":"Department of Otolaryngology,"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Atsushi","family":"Kato","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology,"},{"name":"Division of Allergy and Immunology, Department of Medicine, 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Department of Medicine, and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David B.","family":"Conley","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology,"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kevin C.","family":"Welch","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology,"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert C.","family":"Kern","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology,"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anju T.","family":"Peters","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology,"},{"name":"Division of Allergy and Immunology, Department of Medicine, and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephanie C.","family":"Eisenbarth","sequence":"additional","affiliation":[{"name":"Division of Allergy and Immunology, Department of Medicine, 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