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This crosstalk between the virome and KSC populations remains largely unknown. Here, we investigated the effect of HPV8 on KSCs using various mouse models. We observed that the HPV8 early region gene E6 specifically caused Lrig1\n                    <jats:sup>+<\/jats:sup>\n                    hair follicle junctional zone KSC proliferation and expansion, which would facilitate viral transmission. Within Lrig1\n                    <jats:sup>+<\/jats:sup>\n                    KSCs specifically, HPV8 E6 bound intracellular p300 to phosphorylate the STAT3 transcriptional regulatory node. This induced \u0394Np63 expression, resulting in KSC expansion into the overlying epidermis. HPV8 was associated with 70% of human actinic keratoses. Together, these results define the \u201chit-and-run\u201d mechanism for HPV8 in human actinic keratosis as an expansion of KSCs, which lack melanosome protection and are thus susceptible to sun light\u2013induced malignant transformation.\n                  <\/jats:p>","DOI":"10.1172\/jci.insight.177898","type":"journal-article","created":{"date-parts":[[2024,6,25]],"date-time":"2024-06-25T12:00:41Z","timestamp":1719316841000},"source":"Crossref","is-referenced-by-count":6,"title":["HPV8-induced STAT3 activation led keratinocyte stem cell expansion in human actinic keratoses"],"prefix":"10.1172","volume":"9","author":[{"given":"Huw J.","family":"Morgan","sequence":"first","affiliation":[{"name":"European Cancer Stem Cell Research Institute, School of Biosciences, Cardiff University, Cardiff, United 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