{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T21:52:08Z","timestamp":1789509128662,"version":"build-2803163510"},"reference-count":42,"publisher":"American Society for Clinical Investigation","issue":"5","license":[{"start":{"date-parts":[[2025,3,10]],"date-time":"2025-03-10T00:00:00Z","timestamp":1741564800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000062","name":"NIDDK","doi-asserted-by":"crossref","award":["DK099052,DK118959"],"award-info":[{"award-number":["DK099052,DK118959"]}],"id":[{"id":"10.13039\/100000062","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100000738","name":"Department of Veterans Affairs","doi-asserted-by":"crossref","award":["CX001477-01"],"award-info":[{"award-number":["CX001477-01"]}],"id":[{"id":"10.13039\/100000738","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,3,10]]},"abstract":"<jats:p>\n                    Postinfectious, diarrhea-predominant, irritable bowel syndrome (PI-IBS-D) is difficult to treat owing to its unknown pathophysiology. Extracellular vesicles (EVs) derived from human colon tissue and long noncoding RNAs (lncRNAs), such as growth arrest\u2013specific 5 (GAS5), may play key roles in the pathophysiology of PI-IBS-D. To determine whether altered colonic EV lncRNA signaling leads to gastrointestinal dysfunction and heightened visceral nociception in patients with PI-IBS-D via the GAS5\/miR-23ab\/NMDA NR2B axis, we conducted translational studies, including those on (a) the role of colonic EV lncRNAs in patients with PI-IBS-D, human colonoids, and PI-IBS-D tissues; (b) i.p. injection of colonic EVs from patients with PI-IBS-D into Rab27a\/b\n                    <jats:sup>\u2013\/\u2013<\/jats:sup>\n                    mice (P-EV mice) to investigate whether colonic EVs drive visceral hypersensitivity in vivo via the GAS5\/miR-23ab\/NMDA NR2B axis; and (c) treatment of mice with oligo-miR-23 precursors and anti-GAS5 Vivo-Morpholinos for GAS5\/miR-23ab\/NMDA NR2B axis mechanisms. Colonic EVs from patients with PI-IBS-D, but not from control participants, demonstrated reduced miR-23a\/b expression caused by enhanced GAS5 expression, which drives increased NR2B expression. Intraperitoneal injection of anti\u2013GAS5-Vivo-Morpholino into P-EV mice increased miR-23 levels and decreased NR2B expression and VMR to CD. EVs are internal messengers that alter gastrointestinal function and increase visceral nociception in patients with PI-IBS-D. Strategies to deliver EVs to modulate GAS5\/miR-23ab\/NMDA NR2B axis signaling may lead to new and innovative treatments for patients with PI-IBS-D.\n                  <\/jats:p>","DOI":"10.1172\/jci.insight.178631","type":"journal-article","created":{"date-parts":[[2025,3,9]],"date-time":"2025-03-09T14:00:53Z","timestamp":1741528853000},"source":"Crossref","is-referenced-by-count":5,"title":["Human colonic EVs induce murine enteric neuroplasticity via the lncRNA GAS5\/miR-23\/NMDA NR2B axis"],"prefix":"10.1172","volume":"10","author":[{"given":"QiQi","family":"Zhou","sequence":"first","affiliation":[{"name":"College of Medicine, University of Tennessee Health Science Center, Memphis, Tennessee, USA."},{"name":"Lt. Col. Luke Weathers, Jr. VA Medical Center, Research Service, Memphis, Tennessee, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liuqing","family":"Yang","sequence":"additional","affiliation":[{"name":"College of Medicine, University of Tennessee Health Science Center, Memphis, Tennessee, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zachary T.","family":"Verne","sequence":"additional","affiliation":[{"name":"College of Medicine, University of Tennessee Health Science Center, Memphis, Tennessee, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Benjamin B.","family":"Zhang","sequence":"additional","affiliation":[{"name":"College of Medicine, University of Tennessee Health Science Center, Memphis, Tennessee, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeremy Z.","family":"Fields","sequence":"additional","affiliation":[{"name":"College of Medicine, University of Tennessee Health Science Center, Memphis, Tennessee, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amber T.","family":"Thacker","sequence":"additional","affiliation":[{"name":"College of Medicine, University of Tennessee Health Science Center, Memphis, Tennessee, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G. Nicholas","family":"Verne","sequence":"additional","affiliation":[{"name":"College of Medicine, University of Tennessee Health Science Center, Memphis, Tennessee, USA."},{"name":"Lt. Col. 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