{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,28]],"date-time":"2026-10-28T18:30:18Z","timestamp":1793212218821,"version":"4.5.0"},"reference-count":49,"publisher":"American Society for Clinical Investigation","issue":"9","license":[{"start":{"date-parts":[[2025,5,8]],"date-time":"2025-05-08T00:00:00Z","timestamp":1746662400000},"content-version":"vor","delay-in-days":30,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100002551","name":"Seoul National University","doi-asserted-by":"crossref","award":["800-20220596"],"award-info":[{"award-number":["800-20220596"]}],"id":[{"id":"10.13039\/501100002551","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100016275","name":"Seoul National University Bundang Hospital","doi-asserted-by":"crossref","award":["2019-01164"],"award-info":[{"award-number":["2019-01164"]}],"id":[{"id":"10.13039\/100016275","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100003725","name":"National Research Foundation of Korea","doi-asserted-by":"crossref","award":["NRF-2020R1A2C2013095,NRF-2021R1C1C1003214"],"award-info":[{"award-number":["NRF-2020R1A2C2013095,NRF-2021R1C1C1003214"]}],"id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,5,8]]},"abstract":"<jats:p>Despite the accumulation of cisplatin in proximal tubules, direct visualization of the surrounding peritubular microcirculation, including its change in cisplatin-induced acute kidney injury (AKI), is lacking. Here, using fluorescence and cellular angiography through video-rate high-resolution intravital microscopy, progressive disturbance of peritubular microcirculation in cisplatin-induced AKI in mice was demonstrated. Fluorescence angiography revealed increasing perfusion defects, with a stepwise rise in time to peak (TTP), originating from capillaries surrounding S1 segments. Cellular angiography demonstrated a progressive decrease in the velocity and track length of individual erythrocytes during AKI progression, accompanied by a sequential decrease in the functional capillary ratio (FCR). Changes in the perfusion area, TTP, and FCR preceded significant changes in blood urea nitrogen and cystatin C, suggesting the potential for early diagnosis. Although neutrophil infiltration near proximal tubules increased throughout the progression, it did not cause obstruction of the peritubular microcirculation. Depletion of neutrophils increased mortality due to systemic side effects, whereas functional inactivation of neutrophils using an anti-CD11b antibody improved peritubular microcirculation in cisplatin-induced AKI. This approach enables direct visualization and quantification of peritubular microcirculation and immune cell dynamics, providing insights into renal pathophysiology and potential therapeutic strategies.<\/jats:p>","DOI":"10.1172\/jci.insight.178689","type":"journal-article","created":{"date-parts":[[2025,4,8]],"date-time":"2025-04-08T12:03:38Z","timestamp":1744113818000},"source":"Crossref","is-referenced-by-count":9,"title":["Intravital imaging of peritubular microcirculation impairment in cisplatin-induced acute kidney injury"],"prefix":"10.1172","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7525-9189","authenticated-orcid":true,"given":"Inwon","family":"Park","sequence":"first","affiliation":[{"name":"Department of Emergency Medicine, Seoul National University Bundang Hospital (SNUBH), Seongnam, Republic of Korea."},{"name":"Department of Emergency Medicine, and"},{"name":"Disaster Medicine Research Center, Seoul National University Medical Research Center, Seoul National University College of Medicine, Seoul, Republic of Korea."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Seonghye","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Emergency Medicine, Seoul National University Bundang Hospital (SNUBH), Seongnam, Republic of Korea."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Young Woo","family":"Um","sequence":"additional","affiliation":[{"name":"Department of Emergency Medicine, Seoul National University Bundang Hospital (SNUBH), Seongnam, Republic of Korea."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hee Eun","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Emergency Medicine, Seoul National University Bundang Hospital (SNUBH), Seongnam, Republic of Korea."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jae Hyuk","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Emergency Medicine, Seoul National University Bundang Hospital (SNUBH), Seongnam, Republic of Korea."},{"name":"Department of Emergency Medicine, and"},{"name":"Disaster Medicine Research Center, Seoul National University Medical Research Center, Seoul National University College of Medicine, Seoul, Republic of Korea."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sejoong","family":"Kim","sequence":"additional","affiliation":[{"name":"Division of Nephrology, Department of Internal Medicine, Seoul National University Bundang Hospital, Seongnam, Republic of Korea."},{"name":"Department of Internal Medicine, Seoul National University College of Medicine, Seoul, Republic of Korea."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pilhan","family":"Kim","sequence":"additional","affiliation":[{"name":"Graduate School of Medical Science and Engineering, and"},{"name":"KI for Health Science and Technology (KIHST), Korea Advanced Institute of Science and Technology (KAIST), Daejeon, Republic of Korea."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"You Hwan","family":"Jo","sequence":"additional","affiliation":[{"name":"Department of Emergency Medicine, Seoul National University Bundang Hospital (SNUBH), Seongnam, Republic of Korea."},{"name":"Department of Emergency Medicine, and"},{"name":"Disaster Medicine Research Center, Seoul National University Medical Research Center, Seoul National University College of Medicine, Seoul, Republic of Korea."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"232","published-online":{"date-parts":[[2025,4,8]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.ki.5002786"},{"key":"B2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejphar.2014.07.025"},{"key":"B3","doi-asserted-by":"publisher","DOI":"10.1097\/MAJ.0b013e31812dfe1e"},{"key":"B4","doi-asserted-by":"publisher","DOI":"10.1038\/ki.2008.214"},{"key":"B5","doi-asserted-by":"publisher","DOI":"10.1681\/ASN.2014080741"},{"key":"B6","doi-asserted-by":"publisher","DOI":"10.1681\/ASN.2020101455"},{"key":"B7","doi-asserted-by":"publisher","DOI":"10.1016\/j.kint.2021.06.018"},{"key":"B8","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.00310.2001"},{"key":"B9","doi-asserted-by":"publisher","DOI":"10.1681\/ASN.2013101121"},{"key":"B10","doi-asserted-by":"publisher","DOI":"10.1016\/j.kint.2017.01.009"},{"key":"B11","doi-asserted-by":"publisher","DOI":"10.3390\/toxins2112490"},{"key":"B12","doi-asserted-by":"publisher","DOI":"10.1159\/000503775"},{"key":"B13","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2011.11.004"},{"key":"B14","doi-asserted-by":"publisher","DOI":"10.1016\/j.kint.2021.02.042"},{"key":"B15","doi-asserted-by":"publisher","DOI":"10.1183\/13993003.00786-2018"},{"key":"B16","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4939-7762-8_12"},{"key":"B17","doi-asserted-by":"publisher","DOI":"10.1681\/ASN.2014060577"},{"key":"B18","doi-asserted-by":"publisher","DOI":"10.1681\/ASN.2011020203"},{"key":"B19","doi-asserted-by":"publisher","DOI":"10.14670\/HH-25.33"},{"key":"B20","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbamem.2006.05.012"},{"key":"B21","doi-asserted-by":"publisher","DOI":"10.1016\/j.kint.2017.03.023"},{"key":"B22","doi-asserted-by":"publisher","DOI":"10.1111\/j.1523-1755.2004.66010.x"},{"key":"B23","doi-asserted-by":"publisher","DOI":"10.1155\/2014\/967826"},{"key":"B24","doi-asserted-by":"publisher","DOI":"10.1038\/ki.2012.328"},{"key":"B25","doi-asserted-by":"publisher","DOI":"10.1038\/s41581-022-00539-2"},{"key":"B26","doi-asserted-by":"publisher","DOI":"10.1124\/jpet.107.119792"},{"key":"B27","doi-asserted-by":"publisher","DOI":"10.1038\/nrneph.2014.180"},{"key":"B28","doi-asserted-by":"publisher","DOI":"10.1002\/hep.1840170523"},{"key":"B29","doi-asserted-by":"publisher","DOI":"10.1126\/sciimmunol.aam8929"},{"key":"B30","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bjc.6690047"},{"key":"B31","doi-asserted-by":"publisher","DOI":"10.1038\/nature13165"},{"key":"B32","doi-asserted-by":"publisher","DOI":"10.1681\/ASN.2021070997"},{"key":"B33","doi-asserted-by":"publisher","DOI":"10.1038\/ki.2008.648"},{"key":"B34","doi-asserted-by":"publisher","DOI":"10.3390\/toxins7083245"},{"key":"B35","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajpath.2014.01.023"},{"key":"B36","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0911378107"},{"key":"B37","doi-asserted-by":"publisher","DOI":"10.1097\/MNH.0000000000000219"},{"key":"B38","doi-asserted-by":"publisher","DOI":"10.1159\/000363203"},{"key":"B39","doi-asserted-by":"publisher","DOI":"10.1016\/j.kint.2020.02.011"},{"key":"B40","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-018-19773-5"},{"key":"B41","doi-asserted-by":"publisher","DOI":"10.3390\/cells10051087"},{"key":"B42","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.1440"},{"key":"B43","doi-asserted-by":"publisher","DOI":"10.1038\/s41590-021-01101-1"},{"key":"B44","doi-asserted-by":"publisher","DOI":"10.1155\/2022\/3507721"},{"key":"B45","doi-asserted-by":"publisher","DOI":"10.1364\/BOE.9.002383"},{"key":"B46","doi-asserted-by":"publisher","DOI":"10.1364\/BOE.8.004706"},{"key":"B47","doi-asserted-by":"publisher","DOI":"10.1364\/BOE.6.002158"},{"key":"B48","doi-asserted-by":"publisher","DOI":"10.1080\/19382014.2017.1414764"},{"key":"B49","doi-asserted-by":"publisher","DOI":"10.3791\/61875"}],"container-title":["JCI Insight"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/insight.jci.org\/articles\/view\/178689\/files\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,18]],"date-time":"2026-05-18T17:42:39Z","timestamp":1779126159000},"score":1,"resource":{"primary":{"URL":"https:\/\/insight.jci.org\/articles\/view\/178689"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,4,8]]},"references-count":49,"journal-issue":{"issue":"9","published-online":{"date-parts":[[2025,4,8]]},"published-print":{"date-parts":[[2025,5,8]]}},"alternative-id":["10.1172\/jci.insight.178689"],"URL":"https:\/\/doi.org\/10.1172\/jci.insight.178689","relation":{},"ISSN":["2379-3708"],"issn-type":[{"value":"2379-3708","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,4,8]]},"article-number":"e178689"}}