{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,11]],"date-time":"2026-11-11T14:07:03Z","timestamp":1794406023040,"version":"4.9.0"},"reference-count":44,"publisher":"American Society for Clinical Investigation","issue":"2","license":[{"start":{"date-parts":[[2025,1,23]],"date-time":"2025-01-23T00:00:00Z","timestamp":1737590400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"crossref","award":["18KK0254,19H05654,19H05565,21K19552,23H03027,23H05484,23K19650"],"award-info":[{"award-number":["18KK0254,19H05654,19H05565,21K19552,23H03027,23H05484,23K19650"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100008731","name":"Nakatomi Foundation","doi-asserted-by":"crossref","award":["Nakatomi Foundation Grant"],"award-info":[{"award-number":["Nakatomi Foundation Grant"]}],"id":[{"id":"10.13039\/100008731","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,1,23]]},"abstract":"<jats:p>\n                    Osteoarthritis (OA) shows various clinical manifestations depending on the status of its joint components. We aimed to identify the synovial cell subsets responsible for OA pathophysiology by comprehensive analyses of human synovium samples in single-cell resolution. Two distinct OA synovial tissue groups were classified by gene expression profiles in RNA-Seq: inflammatory and fibrotic. The inflammatory group exhibited high expression of inflammatory cytokines, histologically inflammatory infiltrate, and a more severe pain score. The fibrotic group showed higher expression of fibroblast growth factor (FGFs) and bone morphogenetic proteins (BMPs), showed histologically perivascular fibrosis, and showed a lower pain score. In single-cell RNA-Seq (scRNA-Seq) of synovial cells,\n                    <jats:italic>\n                      MERTK\n                      <jats:sup>lo<\/jats:sup>\n                      CD206\n                      <jats:sup>lo<\/jats:sup>\n                    <\/jats:italic>\n                    macrophages and\n                    <jats:italic>\n                      CD34\n                      <jats:sup>hi<\/jats:sup>\n                    <\/jats:italic>\n                    fibroblasts were associated with the inflammatory and fibrotic groups, respectively. Among the 3 fibroblast subsets,\n                    <jats:italic>\n                      CD34\n                      <jats:sup>lo<\/jats:sup>\n                      THY1\n                      <jats:sup>lo<\/jats:sup>\n                    <\/jats:italic>\n                    and\n                    <jats:italic>\n                      CD34\n                      <jats:sup>lo<\/jats:sup>\n                      THY1\n                      <jats:sup>hi<\/jats:sup>\n                    <\/jats:italic>\n                    fibroblasts were influenced by synovial immune cells, whereas\n                    <jats:italic>\n                      CD34\n                      <jats:sup>hi<\/jats:sup>\n                    <\/jats:italic>\n                    fibroblasts were influenced by mural and endothelial cells. Particularly, in\n                    <jats:italic>\n                      CD34\n                      <jats:sup>hi<\/jats:sup>\n                    <\/jats:italic>\n                    fibroblast subsets,\n                    <jats:italic>\n                      CD34\n                      <jats:sup>hi<\/jats:sup>\n                      CD70\n                      <jats:sup>hi<\/jats:sup>\n                    <\/jats:italic>\n                    fibroblasts promoted proliferation of Tregs, potentially suppressing synovitis and protecting articular cartilage. Elucidation of the mechanisms underlying the regulation of these synovial cell subsets may lead to novel strategies for OA therapeutics.\n                  <\/jats:p>","DOI":"10.1172\/jci.insight.183690","type":"journal-article","created":{"date-parts":[[2025,1,22]],"date-time":"2025-01-22T14:00:48Z","timestamp":1737554448000},"source":"Crossref","is-referenced-by-count":33,"title":["CD34hi subset of synovial fibroblasts contributes to fibrotic phenotype of human knee osteoarthritis"],"prefix":"10.1172","volume":"10","author":[{"given":"Junya","family":"Miyahara","sequence":"first","affiliation":[{"name":"Sensory & Motor System Medicine,"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yasunori","family":"Omata","sequence":"additional","affiliation":[{"name":"Sensory & Motor System Medicine,"},{"name":"Bone and Cartilage Regenerative 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