{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,29]],"date-time":"2026-10-29T06:34:56Z","timestamp":1793255696306,"version":"4.5.0"},"reference-count":77,"publisher":"American Society for Clinical Investigation","issue":"22","license":[{"start":{"date-parts":[[2024,11,22]],"date-time":"2024-11-22T00:00:00Z","timestamp":1732233600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100012659","name":"Foundation for Innovative Research Groups of the National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["82000956"],"award-info":[{"award-number":["82000956"]}],"id":[{"id":"10.13039\/501100012659","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100018543","name":"Natural Science Foundation of Tibet Autonomous Region","doi-asserted-by":"crossref","award":["XZ2023ZR-ZY38(Z)"],"award-info":[{"award-number":["XZ2023ZR-ZY38(Z)"]}],"id":[{"id":"10.13039\/501100018543","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Natural Science Foundation of Shigatse","award":["RKZ2023ZR-001(Z),RKZ2023ZR-008(Z)"],"award-info":[{"award-number":["RKZ2023ZR-001(Z),RKZ2023ZR-008(Z)"]}]},{"name":"Natural Science Foundation of Shanghai","award":["21ZR1411700"],"award-info":[{"award-number":["21ZR1411700"]}]},{"DOI":"10.13039\/501100018543","name":"Natural Science Foundation of Tibet Autonomous Region","doi-asserted-by":"crossref","award":["XZ202401ZR0001"],"award-info":[{"award-number":["XZ202401ZR0001"]}],"id":[{"id":"10.13039\/501100018543","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,11,22]]},"abstract":"<jats:p>\n                    Chronic rhinosinusitis with nasal polyps (CRSwNP) is an inflammatory upper airway disease, divided into eosinophilic CRSwNP (eCRSwNP) and noneosinophilic CRSwNP (neCRSwNP) according to eosinophilic levels. Neutrophils are major effector cells in CRSwNP, but their roles in different inflammatory environments remain largely unclear. We performed an integrated transcriptome analysis of polyp-infiltrating neutrophils from patients with CRSwNP, using healthy donor blood as a control. Additional experiments, including flow cytometry and in vitro epithelial cell and fibroblast culture, were performed to evaluate the phenotypic feature and functional role of neutrophils in CRSwNP. Single-cell RNA-sequencing analysis demonstrated that neutrophils could be classified into 5 functional subsets, with GBP5\n                    <jats:sup>+<\/jats:sup>\n                    neutrophils occurring mainly in neCRSwNP and a high proportion of CXCL8\n                    <jats:sup>+<\/jats:sup>\n                    neutrophils in both subendotypes. GBP5\n                    <jats:sup>+<\/jats:sup>\n                    neutrophils exhibited significant IFN-I pathway activity in neCRSwNP. CXCL8\n                    <jats:sup>+<\/jats:sup>\n                    neutrophils displayed increased neutrophil activation scores and mainly secreted oncostatin M (OSM), which facilitates communication with other cells. In vitro experiments showed that OSM enhanced IL-13\u2013 or IL-17\u2013mediated immune responses in nasal epithelial cells and fibroblasts. Our findings indicate that neutrophils display transcriptional plasticity and activation when exposed to polyp tissue, contributing to CRSwNP pathogenesis by releasing OSM, which interacts with epithelial cells and fibroblasts depending on the inflammatory environment.\n                  <\/jats:p>","DOI":"10.1172\/jci.insight.184739","type":"journal-article","created":{"date-parts":[[2024,10,3]],"date-time":"2024-10-03T12:00:34Z","timestamp":1727956834000},"source":"Crossref","is-referenced-by-count":18,"title":["Neutrophils in nasal polyps exhibit transcriptional adaptation and proinflammatory roles that depend on local polyp milieu"],"prefix":"10.1172","volume":"9","author":[{"given":"Chen","family":"Zhang","sequence":"first","affiliation":[{"name":"ENT Institute and Department of Otorhinolaryngology and"},{"name":"High Altitude Rhinology Research Center, Eye and ENT Hospital, Fudan University, Shanghai, China."},{"name":"Department of Otolaryngology, Shigatse People\u2019s Hospital, Shigatse City, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qianqian","family":"Zhang","sequence":"additional","affiliation":[{"name":"ENT Institute and Department of Otorhinolaryngology and"},{"name":"High Altitude Rhinology Research Center, Eye and ENT Hospital, Fudan University, Shanghai, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiani","family":"Chen","sequence":"additional","affiliation":[{"name":"ENT Institute and Department of Otorhinolaryngology and"},{"name":"High Altitude Rhinology Research Center, Eye and ENT Hospital, Fudan University, Shanghai, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Han","family":"Li","sequence":"additional","affiliation":[{"name":"ENT Institute and Department of Otorhinolaryngology and"},{"name":"High Altitude Rhinology Research Center, Eye and ENT Hospital, Fudan University, Shanghai, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fuying","family":"Cheng","sequence":"additional","affiliation":[{"name":"ENT Institute and Department of Otorhinolaryngology and"},{"name":"High Altitude Rhinology Research Center, Eye and ENT Hospital, Fudan University, Shanghai, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yizhang","family":"Wang","sequence":"additional","affiliation":[{"name":"ENT Institute and Department of Otorhinolaryngology and"},{"name":"High Altitude Rhinology Research Center, Eye and ENT Hospital, Fudan University, Shanghai, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yingqi","family":"Gao","sequence":"additional","affiliation":[{"name":"ENT Institute and Department of Otorhinolaryngology and"},{"name":"High Altitude Rhinology Research Center, Eye and ENT Hospital, Fudan University, Shanghai, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yumin","family":"Zhou","sequence":"additional","affiliation":[{"name":"ENT Institute and Department of Otorhinolaryngology and"},{"name":"High Altitude Rhinology Research Center, Eye and ENT Hospital, Fudan University, Shanghai, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Le","family":"Shi","sequence":"additional","affiliation":[{"name":"ENT Institute and Department of Otorhinolaryngology and"},{"name":"High Altitude Rhinology Research Center, Eye and ENT Hospital, Fudan University, Shanghai, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yufei","family":"Yang","sequence":"additional","affiliation":[{"name":"ENT Institute and Department of Otorhinolaryngology and"},{"name":"High Altitude Rhinology Research Center, Eye and ENT Hospital, Fudan University, Shanghai, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juan","family":"Liu","sequence":"additional","affiliation":[{"name":"ENT Institute and Department of Otorhinolaryngology and"},{"name":"High Altitude Rhinology Research Center, Eye and ENT Hospital, Fudan University, Shanghai, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kai","family":"Xue","sequence":"additional","affiliation":[{"name":"ENT Institute and Department of Otorhinolaryngology and"},{"name":"High Altitude Rhinology Research Center, Eye and ENT Hospital, Fudan University, Shanghai, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yaguang","family":"Zhang","sequence":"additional","affiliation":[{"name":"Med-X Institute, Center for Immunological and Metabolic Diseases, The First Affiliated Hospital of Xi\u2019an JiaoTong University, Xi\u2019an JiaoTong University, Xi\u2019an, Shaanxi, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongmeng","family":"Yu","sequence":"additional","affiliation":[{"name":"ENT Institute and Department of Otorhinolaryngology and"},{"name":"High Altitude Rhinology Research Center, Eye and ENT Hospital, Fudan University, Shanghai, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dehui","family":"Wang","sequence":"additional","affiliation":[{"name":"ENT Institute and Department of Otorhinolaryngology and"},{"name":"High Altitude Rhinology Research Center, Eye and ENT Hospital, Fudan University, Shanghai, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li","family":"Hu","sequence":"additional","affiliation":[{"name":"ENT Institute and Department of Otorhinolaryngology and"},{"name":"High Altitude Rhinology Research Center, Eye and ENT Hospital, Fudan University, Shanghai, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huan","family":"Wang","sequence":"additional","affiliation":[{"name":"ENT Institute and Department of Otorhinolaryngology and"},{"name":"High Altitude Rhinology Research Center, Eye and ENT Hospital, Fudan University, Shanghai, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xicai","family":"Sun","sequence":"additional","affiliation":[{"name":"ENT Institute and Department of Otorhinolaryngology and"},{"name":"High Altitude Rhinology Research Center, Eye and ENT Hospital, Fudan University, Shanghai, China."},{"name":"Department of Otolaryngology, Shigatse People\u2019s Hospital, Shigatse City, China."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"232","published-online":{"date-parts":[[2024,11,22]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","DOI":"10.4193\/Rhin20.401"},{"key":"B2","doi-asserted-by":"publisher","DOI":"10.1002\/alr.22741"},{"key":"B3","doi-asserted-by":"publisher","DOI":"10.1111\/all.15074"},{"key":"B4","doi-asserted-by":"publisher","DOI":"10.1111\/cea.14065"},{"key":"B5","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2022.10.010"},{"key":"B6","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2021.11.023"},{"key":"B7","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2020.08.036"},{"key":"B8","doi-asserted-by":"publisher","DOI":"10.1111\/all.14071"},{"key":"B9","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2023.11.016"},{"key":"B10","doi-asserted-by":"publisher","DOI":"10.1111\/all.12064"},{"key":"B11","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2024.02.005"},{"key":"B12","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2023.112525"},{"key":"B13","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-022-05400-x"},{"key":"B14","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.2200154"},{"key":"B15","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2022.12.679"},{"key":"B16","doi-asserted-by":"publisher","DOI":"10.1189\/jlb.0612273"},{"key":"B17","doi-asserted-by":"publisher","DOI":"10.1136\/gutjnl-2021-326070"},{"key":"B18","doi-asserted-by":"publisher","DOI":"10.1038\/s41590-020-0736-z"},{"key":"B19","doi-asserted-by":"publisher","DOI":"10.1093\/rheumatology\/keaa167"},{"key":"B20","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2015.01.043"},{"key":"B21","doi-asserted-by":"publisher","DOI":"10.1126\/scitranslmed.abf8188"},{"key":"B22","doi-asserted-by":"publisher","DOI":"10.1016\/j.alit.2023.07.004"},{"key":"B23","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2019.09.029"},{"key":"B24","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2017.12.972"},{"key":"B25","doi-asserted-by":"publisher","DOI":"10.1111\/all.13919"},{"key":"B26","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2020.01.009"},{"key":"B27","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2018.08.049"},{"key":"B28","doi-asserted-by":"publisher","DOI":"10.1111\/all.15384"},{"key":"B29","doi-asserted-by":"publisher","DOI":"10.1038\/nature15367"},{"key":"B30","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-pathmechdis-051222-015009"},{"key":"B31","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2003603117"},{"key":"B32","doi-asserted-by":"publisher","DOI":"10.1111\/cei.13525"},{"key":"B33","doi-asserted-by":"publisher","DOI":"10.1186\/s13578-023-01188-z"},{"key":"B34","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2012.01.079"},{"key":"B35","doi-asserted-by":"publisher","DOI":"10.1002\/wjo2.91"},{"key":"B36","doi-asserted-by":"publisher","DOI":"10.1126\/scitranslmed.adf3843"},{"key":"B37","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2019.01.042"},{"key":"B38","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2019.08.027"},{"key":"B39","doi-asserted-by":"publisher","DOI":"10.1002\/clt2.12269"},{"key":"B40","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2022.12.826"},{"key":"B41","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2024.04.020"},{"key":"B42","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-021-21246-9"},{"key":"B43","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2016.10.039"},{"key":"B44","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2022.11.029"},{"key":"B45","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2019.06.037"},{"key":"B46","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2015.09.005"},{"key":"B47","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2016.07.020"},{"key":"B48","doi-asserted-by":"publisher","DOI":"10.4168\/aair.2020.12.3.507"},{"key":"B49","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2020.11.037"},{"key":"B50","doi-asserted-by":"publisher","DOI":"10.1186\/s12931-014-0164-4"},{"key":"B51","doi-asserted-by":"publisher","DOI":"10.1165\/rcmb.2012-0460OC"},{"key":"B52","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1912702117"},{"key":"B53","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1801377115"},{"key":"B54","doi-asserted-by":"publisher","DOI":"10.1016\/j.chom.2023.08.016"},{"key":"B55","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2022.04.140"},{"issue":"4","key":"B56","first-page":"285","article-title":"Lund-Mackay score is predictive of bleeding in ethmoidectomy for nasal polyposis","volume":"46","author":"Mortuaire","year":"2008","journal-title":"Rhinology"},{"key":"B57","doi-asserted-by":"publisher","DOI":"10.1038\/s41590-022-01312-0"},{"key":"B58","doi-asserted-by":"publisher","DOI":"10.1038\/s41591-021-01576-3"},{"key":"B59","unstructured":"10x Genomics. Capturing Neutrophils in 10x Single Cell Gene Expression Data. https:\/\/support.10xgenomics.com\/single-cell-gene-expression\/software\/pipelines\/latest\/tutorials\/neutrophils Accessed October 17, 2024"},{"key":"B60","doi-asserted-by":"publisher","DOI":"10.1038\/nbt.4096"},{"key":"B61","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2021.04.048"},{"key":"B62","doi-asserted-by":"publisher","DOI":"10.1186\/s13059-019-1874-1"},{"key":"B63","doi-asserted-by":"publisher","DOI":"10.1038\/s41592-019-0619-0"},{"key":"B64","doi-asserted-by":"publisher","DOI":"10.1093\/gigascience\/giy083"},{"key":"B65","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-018-0449-8"},{"key":"B66","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2164-14-632"},{"key":"B67","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-018-0694-x"},{"key":"B68","doi-asserted-by":"publisher","DOI":"10.1038\/nbt.2859"},{"key":"B69","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkac1000"},{"key":"B70","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2007.324"},{"key":"B71","doi-asserted-by":"publisher","DOI":"10.1016\/j.xinn.2021.100141"},{"key":"B72","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-022-32552-1"},{"key":"B73","doi-asserted-by":"publisher","DOI":"10.1038\/ng.3593"},{"key":"B74","doi-asserted-by":"publisher","DOI":"10.1186\/s13059-020-1949-z"},{"key":"B75","doi-asserted-by":"publisher","DOI":"10.1172\/JCI121087"},{"key":"B76","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btw313"},{"key":"B77","doi-asserted-by":"publisher","DOI":"10.1007\/s00405-023-07905-1"}],"container-title":["JCI Insight"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/insight.jci.org\/articles\/view\/184739\/files\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,18]],"date-time":"2026-05-18T20:37:43Z","timestamp":1779136663000},"score":1,"resource":{"primary":{"URL":"https:\/\/insight.jci.org\/articles\/view\/184739"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,11,22]]},"references-count":77,"journal-issue":{"issue":"22","published-online":{"date-parts":[[2024,11,22]]},"published-print":{"date-parts":[[2024,11,22]]}},"alternative-id":["10.1172\/jci.insight.184739"],"URL":"https:\/\/doi.org\/10.1172\/jci.insight.184739","relation":{},"ISSN":["2379-3708"],"issn-type":[{"value":"2379-3708","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,11,22]]},"article-number":"e184739"}}