{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T20:30:18Z","timestamp":1792701018828,"version":"4.5.0"},"reference-count":41,"publisher":"American Society for Clinical Investigation","issue":"7","license":[{"start":{"date-parts":[[2025,4,8]],"date-time":"2025-04-08T00:00:00Z","timestamp":1744070400000},"content-version":"vor","delay-in-days":42,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001381","name":"National Research Foundation of Singapore","doi-asserted-by":"crossref","award":["NRF-CRP26-2021RS-0001,NRF2017_SISFP09"],"award-info":[{"award-number":["NRF-CRP26-2021RS-0001,NRF2017_SISFP09"]}],"id":[{"id":"10.13039\/501100001381","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001349","name":"National Medical Research Council","doi-asserted-by":"crossref","award":["NMRC\/OFLCG\/003\/2018"],"award-info":[{"award-number":["NMRC\/OFLCG\/003\/2018"]}],"id":[{"id":"10.13039\/501100001349","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001349","name":"National Medical Research Council","doi-asserted-by":"crossref","award":["OFIRG24jan-0111"],"award-info":[{"award-number":["OFIRG24jan-0111"]}],"id":[{"id":"10.13039\/501100001349","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,4,8]]},"abstract":"<jats:p>\n                    The functional plasticity of tumor-infiltrating lymphocyte B\u2013cells (TIL-B) spans from antitumor responses to noncanonical immune suppression. Yet, how the tumor microenvironment (TME) influences TIL-B development is still underappreciated. Our current study integrated single-cell transcriptomics and B cell receptor (BCR) sequencing to profile TIL-B phenotypes and clonalities in hepatocellular carcinoma (HCC). Using trajectory and gene regulatory network analysis, we were able to characterize plasma cells and memory and naive B cells within the HCC TME and further revealed a downregulation of BCR signaling genes in plasma cells and a subset of inflammatory TNF\n                    <jats:sup>+<\/jats:sup>\n                    memory B cells. Within the TME, a nonswitched memory B cell subset acquired an age-associated B cell phenotype (TBET\n                    <jats:sup>+<\/jats:sup>\n                    CD11c\n                    <jats:sup>+<\/jats:sup>\n                    ) and expressed higher levels of PD-L1, CD25, and granzyme B. We further demonstrated that the presence of HCC tumor cells could confer suppressive functions on peripheral blood B cells that in turn, dampen T cell costimulation. To the best of our knowledge, these findings represent novel mechanisms of noncanonical immune suppression in HCC. While previous studies identified atypical memory B cells in chronic hepatitis and across several solid cancer types, we further highlighted their potential role as regulatory B cells (Bregs) within both the TME and peripheral blood of HCC patients.\n                  <\/jats:p>","DOI":"10.1172\/jci.insight.187025","type":"journal-article","created":{"date-parts":[[2025,2,25]],"date-time":"2025-02-25T12:00:37Z","timestamp":1740484837000},"source":"Crossref","is-referenced-by-count":16,"title":["Atypical memory B cells acquire Breg phenotypes in hepatocellular carcinoma"],"prefix":"10.1172","volume":"10","author":[{"given":"Shi Yong","family":"Neo","sequence":"first","affiliation":[{"name":"Singapore Immunology Network (SIgN), Agency for Science, Technology and Research (A*STAR), Singapore."},{"name":"Department of Oncology and Pathology, Karolinska Institute, Stockholm, Sweden."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Timothy Wai 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