{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,25]],"date-time":"2026-08-25T04:24:39Z","timestamp":1787631879450,"version":"build-2736575974"},"reference-count":78,"publisher":"American Society for Clinical Investigation","issue":"13","license":[{"start":{"date-parts":[[2025,7,8]],"date-time":"2025-07-08T00:00:00Z","timestamp":1751932800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000050","name":"National Heart, Lung, and Blood Institute","doi-asserted-by":"crossref","award":["R01HL148718"],"award-info":[{"award-number":["R01HL148718"]}],"id":[{"id":"10.13039\/100000050","id-type":"DOI","asserted-by":"crossref"}]},{"name":"National Cancer Institute","award":["P30CA013696"],"award-info":[{"award-number":["P30CA013696"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,7,8]]},"abstract":"<jats:p>\n                    Regulatory T cells (Tregs) are known to play critical roles in tissue repair via provision of growth factors, such as amphiregulin (Areg). Areg-producing Tregs have previously been difficult to study because of an inability to isolate live Areg-producing cells. In this report, we created a reporter mouse to detect Areg expression in live cells (\n                    <jats:italic>\n                      Areg\n                      <jats:sup>Thy1.1<\/jats:sup>\n                    <\/jats:italic>\n                    ). We employed influenza A and bleomycin models of lung damage to sort Areg-producing and non-Areg-producing Tregs for transcriptomic analyses. Single-cell RNA-Seq revealed distinct subpopulations of Tregs and allowed transcriptomic comparisons of damage-induced populations. Single-cell TCR sequencing showed that Treg clonal expansion was biased toward Areg-producing Tregs and largely occurred within damage-induced subgroups. Gene module analysis revealed functional divergence of Tregs into immunosuppression-oriented and tissue repair\u2013oriented groups, leading to identification of candidate receptors for induction of repair activity in Tregs. We tested these using an ex vivo assay for Treg-mediated tissue repair, identifying 4-1BB agonism as a mechanism for reparative activity induction. Overall, we demonstrate that the\n                    <jats:italic>\n                      Areg\n                      <jats:sup>Thy1.1<\/jats:sup>\n                    <\/jats:italic>\n                    mouse is a promising tool for investigating tissue repair activity in leukocytes.\n                  <\/jats:p>","DOI":"10.1172\/jci.insight.187245","type":"journal-article","created":{"date-parts":[[2025,7,7]],"date-time":"2025-07-07T14:00:32Z","timestamp":1751896832000},"source":"Crossref","is-referenced-by-count":4,"title":["An amphiregulin reporter mouse enables transcriptional and clonal expansion analysis of reparative lung Tregs"],"prefix":"10.1172","volume":"10","author":[{"given":"Lucas F.","family":"Loffredo","sequence":"first","affiliation":[{"name":"Department of Microbiology & Immunology;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katherine A.","family":"Kaiser","sequence":"additional","affiliation":[{"name":"Department of Microbiology & Immunology;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adam","family":"Kornberg","sequence":"additional","affiliation":[{"name":"Department of Microbiology & Immunology;"},{"name":"Columbia Center for Translational Immunology;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Samhita","family":"Rao","sequence":"additional","affiliation":[{"name":"Department of Microbiology & Immunology;"},{"name":"Columbia Center for Translational Immunology;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kenia","family":"de los Santos-Alexis","sequence":"additional","affiliation":[{"name":"Department of Microbiology & Immunology;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arnold","family":"Han","sequence":"additional","affiliation":[{"name":"Columbia Center for Translational Immunology;"},{"name":"Division of Digestive and Liver Diseases, Department of Medicine; 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