{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T20:31:09Z","timestamp":1790800269516,"version":"4.1.0"},"reference-count":62,"publisher":"American Society for Clinical Investigation","issue":"12","license":[{"start":{"date-parts":[[2025,6,23]],"date-time":"2025-06-23T00:00:00Z","timestamp":1750636800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000052","name":"NIH Office of the Director","doi-asserted-by":"crossref","award":["R01AR065807,R01AI127654,DP2CA247831,U2C-CA233262"],"award-info":[{"award-number":["R01AR065807,R01AI127654,DP2CA247831,U2C-CA233262"]}],"id":[{"id":"10.13039\/100000052","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,6,23]]},"abstract":"<jats:p>\n                    Tissue-resident memory T (T\n                    <jats:sub>RM<\/jats:sub>\n                    ) cells play a central role in immune responses across all barrier tissues after infection. However, the mechanisms that drive T\n                    <jats:sub>RM<\/jats:sub>\n                    differentiation and priming for their recall effector function remains unclear. In this study, we leveraged newly generated and publicly available single-cell RNA-seq data generated across 10 developmental time points to define features of CD8\n                    <jats:sup>+<\/jats:sup>\n                    T\n                    <jats:sub>RM<\/jats:sub>\n                    across both skin and small-intestine intraepithelial lymphocytes (siIEL). We employed linear modeling to capture gene programs that increase their expression levels in T cells transitioning from an effector to a memory state. In addition to capturing tissue-specific gene programs, we defined a temporal T\n                    <jats:sub>RM<\/jats:sub>\n                    signature across skin and siIEL that can distinguish T\n                    <jats:sub>RM<\/jats:sub>\n                    from circulating T cell populations. This T\n                    <jats:sub>RM<\/jats:sub>\n                    signature highlights biology that is missed in published signatures that compared bulk T\n                    <jats:sub>RM<\/jats:sub>\n                    to naive or nontissue resident memory populations. This temporal T\n                    <jats:sub>RM<\/jats:sub>\n                    signature included the AP-1 transcription factor family members\n                    <jats:italic>Fos, Fosb,<\/jats:italic>\n                    <jats:italic>Fosl2<\/jats:italic>\n                    , and\n                    <jats:italic>Junb<\/jats:italic>\n                    . ATAC-seq analysis detected AP-1\u2013specific motifs at open chromatin sites in mature T\n                    <jats:sub>RM<\/jats:sub>\n                    . Cyclic immunofluorescence (CyCIF) tissue imaging detected nuclear colocalization of AP-1 members in resting CD8\n                    <jats:sup>+<\/jats:sup>\n                    T\n                    <jats:sub>RM<\/jats:sub>\n                    greater than 100 days after infection. Taken together, these results reveal a critical role of AP-1 transcription factor members in T\n                    <jats:sub>RM<\/jats:sub>\n                    biology.\n                  <\/jats:p>","DOI":"10.1172\/jci.insight.187381","type":"journal-article","created":{"date-parts":[[2025,6,22]],"date-time":"2025-06-22T14:00:26Z","timestamp":1750600826000},"source":"Crossref","is-referenced-by-count":10,"title":["Resident memory T cell development is gradual and shows AP-1 gene expression in mature cells"],"prefix":"10.1172","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1394-3158","authenticated-orcid":true,"given":"Neal P.","family":"Smith","sequence":"first","affiliation":[{"name":"Center for Immunology and Inflammatory Diseases, Department of Medicine, Massachusetts General Hospital, Boston, Massachusetts, USA."},{"name":"Massachusetts General Hospital, Cancer Center, Boston, Massachusetts, USA."},{"name":"Broad Institute of Massachusetts Institute of 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Hospital, Boston, Massachusetts, USA."},{"name":"Harvard Medical School, Boston, Massachusetts, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tian","family":"Tian","sequence":"additional","affiliation":[{"name":"Department of Dermatology, Brigham and Women\u2019s Hospital, Boston, Massachusetts, USA."},{"name":"Harvard Medical School, Boston, Massachusetts, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Timothy","family":"Pan","sequence":"additional","affiliation":[{"name":"Department of Dermatology, Brigham and Women\u2019s Hospital, Boston, Massachusetts, USA."},{"name":"Harvard Medical School, Boston, Massachusetts, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claire","family":"Stingley","sequence":"additional","affiliation":[{"name":"Department of Dermatology, Brigham and Women\u2019s Hospital, Boston, Massachusetts, USA."},{"name":"Harvard Medical School, Boston, Massachusetts, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kevin","family":"Wu","sequence":"additional","affiliation":[{"name":"Department of Dermatology, Brigham and Women\u2019s Hospital, Boston, Massachusetts, USA."},{"name":"Harvard Medical School, Boston, Massachusetts, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiang","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Dermatology, Brigham and Women\u2019s Hospital, Boston, Massachusetts, USA."},{"name":"Harvard Medical School, Boston, Massachusetts, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexander L.","family":"Kley","sequence":"additional","affiliation":[{"name":"Department of Dermatology, Brigham and Women\u2019s Hospital, Boston, Massachusetts, USA."},{"name":"Harvard Medical School, Boston, Massachusetts, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter 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