{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,4]],"date-time":"2026-06-04T06:26:31Z","timestamp":1780554391501,"version":"3.54.1"},"reference-count":74,"publisher":"American Society for Clinical Investigation","issue":"24","license":[{"start":{"date-parts":[[2025,12,22]],"date-time":"2025-12-22T00:00:00Z","timestamp":1766361600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"crossref","award":["R01AR074310,P50AR060804,R01AR073855,U54GM104938,UM1AI144292,P30AR073750"],"award-info":[{"award-number":["R01AR074310,P50AR060804,R01AR073855,U54GM104938,UM1AI144292,P30AR073750"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100006260","name":"Rheumatology Research Foundation","doi-asserted-by":"crossref","award":["Innovative Research Award"],"award-info":[{"award-number":["Innovative Research Award"]}],"id":[{"id":"10.13039\/100006260","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,12,22]]},"abstract":"<jats:p>\n                    CD4\n                    <jats:sup>+<\/jats:sup>\n                    T cells predominate lymphocytic foci found in the salivary glands (SGs) of Sj\u00f6gren\u2019s disease (SjD) cases. Yet little is known about T cell receptor (TCR) repertoire features that distinguish cases from healthy controls (HCs), the relationship between SG and peripheral blood (PB) repertoires of cases, and antigens recognized by pathogenic T cell clones. We performed deep sequencing of bulk-sorted CD4\n                    <jats:sup>+<\/jats:sup>\n                    CD45RA\n                    <jats:sup>\u2013<\/jats:sup>\n                    PB T cells from SjD cases and matched HCs, and single-cell TCR sequencing of the same T cell population from labial SG biopsies of these cases. We found that clonally expanded SG CD4\n                    <jats:sup>+<\/jats:sup>\n                    T cells expressed complementarity-determining region 3 (CDR3) sequences that were also detected in multiple copies in the blood of the same individuals with SjD. SjD cases displayed a \u201cprivate\u201d and restricted PB TCR repertoire with reduced clonotype diversity. We identified SjD-associated TCR motifs with the same putative antigen specificity shared between SGs and PB of cases. Their abundances in PB correlated with reduced salivary flow, linking these T cells with pathogenic disease features. Finally, we discovered 2 Ro60 epitopes eliciting an HLA-restricted immune response from expanded SG T cell clones. The comprehensive characterization of SjD TCR repertoires enables the discovery of target antigens and therapeutic strategies.\n                  <\/jats:p>","DOI":"10.1172\/jci.insight.188496","type":"journal-article","created":{"date-parts":[[2025,12,21]],"date-time":"2025-12-21T19:00:44Z","timestamp":1766343644000},"source":"Crossref","is-referenced-by-count":2,"title":["Identification of Sj\u00f6gren\u2019s disease\u2013associated T cell receptor motifs through deep sequencing"],"prefix":"10.1172","volume":"10","author":[{"given":"Ananth Aditya","family":"Jupudi","sequence":"first","affiliation":[{"name":"Arthritis and Clinical Immunology Program, Oklahoma Medical Research Foundation, Oklahoma City, Oklahoma, USA."},{"name":"Department of Microbiology and Immunology, University of Oklahoma Health Sciences Center, Oklahoma City, Oklahoma, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7673-798X","authenticated-orcid":true,"given":"Michelle L.","family":"Joachims","sequence":"additional","affiliation":[{"name":"Arthritis and Clinical Immunology Program, Oklahoma Medical Research Foundation, Oklahoma City, Oklahoma, USA."},{"name":"Genes and Human Disease Program, Oklahoma Medical Research Foundation, Oklahoma City, Oklahoma, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christina","family":"Lawrence","sequence":"additional","affiliation":[{"name":"Arthritis and Clinical Immunology Program, Oklahoma Medical Research Foundation, Oklahoma City, Oklahoma, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Charmaine","family":"Lopez-Davis","sequence":"additional","affiliation":[{"name":"Arthritis and Clinical Immunology Program, Oklahoma Medical Research Foundation, Oklahoma City, Oklahoma, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bhuwan","family":"Khatri","sequence":"additional","affiliation":[{"name":"Genes and Human Disease Program, Oklahoma Medical Research Foundation, Oklahoma City, Oklahoma, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7744-2948","authenticated-orcid":true,"given":"Astrid","family":"Rasmussen","sequence":"additional","affiliation":[{"name":"Genes and Human Disease Program, Oklahoma Medical Research Foundation, Oklahoma City, Oklahoma, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kiely","family":"Grundahl","sequence":"additional","affiliation":[{"name":"Genes and Human Disease Program, Oklahoma Medical Research Foundation, Oklahoma City, Oklahoma, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R. Hal","family":"Scofield","sequence":"additional","affiliation":[{"name":"Arthritis and Clinical Immunology Program, Oklahoma Medical Research Foundation, Oklahoma City, Oklahoma, USA."},{"name":"Department of Medicine, University of Oklahoma Health Sciences Center, Oklahoma City, Oklahoma, USA."},{"name":"Department of Veteran\u2019s Affairs Medical Center, Oklahoma City, Oklahoma, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9574-7355","authenticated-orcid":true,"given":"Judith A.","family":"James","sequence":"additional","affiliation":[{"name":"Arthritis and Clinical Immunology Program, Oklahoma Medical Research Foundation, Oklahoma City, Oklahoma, USA."},{"name":"Department of Medicine, University of Oklahoma Health Sciences Center, Oklahoma City, Oklahoma, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9308-237X","authenticated-orcid":true,"given":"Joel M.","family":"Guthridge","sequence":"additional","affiliation":[{"name":"Arthritis and Clinical Immunology Program, Oklahoma Medical Research Foundation, Oklahoma City, Oklahoma, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christopher J.","family":"Lessard","sequence":"additional","affiliation":[{"name":"Genes and Human Disease Program, Oklahoma Medical Research Foundation, Oklahoma City, Oklahoma, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Linda F.","family":"Thompson","sequence":"additional","affiliation":[{"name":"Arthritis and Clinical Immunology Program, Oklahoma Medical Research Foundation, Oklahoma City, Oklahoma, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3009-2017","authenticated-orcid":true,"given":"A. Darise","family":"Farris","sequence":"additional","affiliation":[{"name":"Arthritis and Clinical Immunology Program, Oklahoma Medical Research Foundation, Oklahoma City, Oklahoma, USA."},{"name":"Department of Microbiology and Immunology, University of Oklahoma Health Sciences Center, Oklahoma City, Oklahoma, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"232","published-online":{"date-parts":[[2025,12,22]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","DOI":"10.1038\/nrdp.2016.47"},{"key":"B2","doi-asserted-by":"publisher","DOI":"10.1136\/annrheumdis-2014-206418"},{"key":"B3","doi-asserted-by":"publisher","DOI":"10.1007\/s10067-020-05004-8"},{"key":"B4","doi-asserted-by":"publisher","DOI":"10.3899\/jrheum.090804"},{"key":"B5","doi-asserted-by":"publisher","DOI":"10.1093\/rheumatology\/kel252"},{"key":"B6","doi-asserted-by":"publisher","DOI":"10.1016\/j.autrev.2015.03.008"},{"key":"B7","doi-asserted-by":"publisher","DOI":"10.3109\/03009742.2014.931457"},{"key":"B8","doi-asserted-by":"publisher","DOI":"10.1001\/archinte.164.12.1275"},{"key":"B9","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaut.2014.02.008"},{"key":"B10","doi-asserted-by":"publisher","DOI":"10.1038\/ng.2792"},{"key":"B11","doi-asserted-by":"publisher","DOI":"10.1038\/nrrheum.2010.118"},{"key":"B12","doi-asserted-by":"publisher","DOI":"10.3390\/jcm9072164"},{"key":"B13","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.immunol.23.021704.115658"},{"key":"B14","doi-asserted-by":"publisher","DOI":"10.1172\/jci.insight.85609"},{"key":"B15","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2021.639672"},{"key":"B16","doi-asserted-by":"publisher","DOI":"10.1172\/JCI117561"},{"key":"B17","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1098-2825(1998)12:3<162::AID-JCLA6>3.0.CO;2-B"},{"issue":"10","key":"B18","first-page":"2510","article-title":"T cell receptor repertoire of infiltrating T cells in the interstitial nephritis of patients with Sj\u00f6gren\u2019s syndrome","volume":"53","author":"Murata","year":"1995","journal-title":"Nihon Rinsho"},{"key":"B19","doi-asserted-by":"publisher","DOI":"10.3892\/ijmm.4.5.519"},{"key":"B20","doi-asserted-by":"publisher","DOI":"10.1172\/JCI118629"},{"key":"B21","doi-asserted-by":"publisher","DOI":"10.1016\/j.ebiom.2022.104252"},{"key":"B22","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.2100803"},{"key":"B23","doi-asserted-by":"publisher","DOI":"10.1038\/s41587-020-0505-4"},{"key":"B24","doi-asserted-by":"publisher","DOI":"10.1002\/art.1780290207"},{"key":"B25","doi-asserted-by":"publisher","DOI":"10.1002\/art.39859"},{"key":"B26","doi-asserted-by":"publisher","DOI":"10.1093\/oxfordjournals.aje.a010064"},{"key":"B27","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2022.858057"},{"key":"B28","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.68605"},{"key":"B29","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-021-25006-7"},{"key":"B30","doi-asserted-by":"publisher","DOI":"10.1038\/nature22976"},{"key":"B31","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2021.664514"},{"key":"B32","doi-asserted-by":"publisher","DOI":"10.1136\/ard.2009.110619"},{"key":"B33","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2018.00462"},{"key":"B34","doi-asserted-by":"publisher","DOI":"10.1111\/imr.12665"},{"key":"B35","doi-asserted-by":"publisher","DOI":"10.1038\/nri2260"},{"key":"B36","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1010586107"},{"key":"B37","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2020.06.024"},{"key":"B38","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btz035"},{"key":"B39","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btz614"},{"key":"B40","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btx286"},{"key":"B41","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkx760"},{"key":"B42","doi-asserted-by":"publisher","DOI":"10.1084\/jem.177.1.119"},{"key":"B43","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.135.5.3542"},{"key":"B44","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2023.10.024"},{"key":"B45","doi-asserted-by":"publisher","DOI":"10.2147\/JIR.S137024"},{"key":"B46","doi-asserted-by":"publisher","DOI":"10.2337\/db16-1025"},{"key":"B47","doi-asserted-by":"publisher","DOI":"10.36069\/jols\/20201203"},{"key":"B48","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2013.00456"},{"key":"B49","doi-asserted-by":"publisher","DOI":"10.1186\/ar2553"},{"key":"B50","doi-asserted-by":"publisher","DOI":"10.1093\/intimm\/dxt037"},{"key":"B51","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1402724111"},{"key":"B52","doi-asserted-by":"publisher","DOI":"10.1038\/nri3279"},{"key":"B53","doi-asserted-by":"publisher","DOI":"10.1385\/IR:30:2:231"},{"key":"B54","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M111.289488"},{"key":"B55","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-022-05501-7"},{"issue":"4","key":"B56","first-page":"47","article-title":"Serious infections in Sj\u00f6gren\u2019s syndrome patients: a national U.S. study","volume":"38 Suppl 126","author":"Singh","year":"2020","journal-title":"Clin Exp Rheumatol"},{"key":"B57","doi-asserted-by":"publisher","DOI":"10.1080\/07853890.2022.2126517"},{"key":"B58","doi-asserted-by":"publisher","DOI":"10.1183\/16000617.0011-2016"},{"key":"B59","doi-asserted-by":"publisher","DOI":"10.1136\/bjo.2003.028340"},{"key":"B60","doi-asserted-by":"publisher","DOI":"10.1002\/art.1780310406"},{"key":"B61","doi-asserted-by":"publisher","DOI":"10.1002\/art.42122"},{"key":"B62","doi-asserted-by":"publisher","DOI":"10.1136\/ard-2022-223105"},{"key":"B63","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20111485"},{"key":"B64","doi-asserted-by":"publisher","DOI":"10.1136\/annrheumdis-2013-203845"},{"key":"B65","doi-asserted-by":"publisher","DOI":"10.1038\/ng.3656"},{"key":"B66","doi-asserted-by":"publisher","DOI":"10.1002\/art.38872"},{"key":"B67","doi-asserted-by":"publisher","DOI":"10.1038\/icb.2016.16"},{"key":"B68","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1500215"},{"key":"B69","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0018556"},{"key":"B70","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.1318"},{"key":"B71","doi-asserted-by":"publisher","DOI":"10.1016\/j.jim.2018.08.011"},{"key":"B72","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1109438108"},{"key":"B73","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2013.111"},{"key":"B74","doi-asserted-by":"publisher","DOI":"10.2337\/db11-1113"}],"container-title":["JCI Insight"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/insight.jci.org\/articles\/view\/188496\/files\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,17]],"date-time":"2026-05-17T14:56:21Z","timestamp":1779029781000},"score":1,"resource":{"primary":{"URL":"https:\/\/insight.jci.org\/articles\/view\/188496"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,12,22]]},"references-count":74,"journal-issue":{"issue":"24","published-online":{"date-parts":[[2025,12,22]]},"published-print":{"date-parts":[[2025,12,22]]}},"alternative-id":["10.1172\/jci.insight.188496"],"URL":"https:\/\/doi.org\/10.1172\/jci.insight.188496","relation":{},"ISSN":["2379-3708"],"issn-type":[{"value":"2379-3708","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,12,22]]},"article-number":"e188496"}}