{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,5]],"date-time":"2026-11-05T01:54:17Z","timestamp":1793843657488,"version":"4.6.1"},"reference-count":62,"publisher":"American Society for Clinical Investigation","issue":"14","license":[{"start":{"date-parts":[[2025,7,22]],"date-time":"2025-07-22T00:00:00Z","timestamp":1753142400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100002736","name":"CURE Epilepsy","doi-asserted-by":"crossref","award":["Taking Flight Award"],"award-info":[{"award-number":["Taking Flight Award"]}],"id":[{"id":"10.13039\/100002736","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Science Foundation Ireland","award":["SIRG\/19\/5646"],"award-info":[{"award-number":["SIRG\/19\/5646"]}]},{"name":"Science Foundation Ireland","award":["16\/RC\/3948"],"award-info":[{"award-number":["16\/RC\/3948"]}]},{"name":"Science Foundation Ireland","award":["21\/RC\/10294"],"award-info":[{"award-number":["21\/RC\/10294"]}]},{"DOI":"10.13039\/100010414","name":"Health Research Board","doi-asserted-by":"crossref","award":["ILP-POR-2024-034"],"award-info":[{"award-number":["ILP-POR-2024-034"]}],"id":[{"id":"10.13039\/100010414","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100018694","name":"HORIZON EUROPE Marie Sklodowska-Curie Actions","doi-asserted-by":"crossref","award":["MSCA-IF-101062165"],"award-info":[{"award-number":["MSCA-IF-101062165"]}],"id":[{"id":"10.13039\/100018694","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,7,22]]},"abstract":"<jats:p>\n                    Analogous to DNA methylation and protein phosphorylation, it is now well understood that RNA is also subject to extensive processing and modification.\n                    <jats:italic>N<\/jats:italic>\n                    <jats:sup>6<\/jats:sup>\n                    -methyladenosine (m\n                    <jats:sup>6<\/jats:sup>\n                    A) is the most abundant internal RNA modification and regulates RNA fate in several ways, including stability and translational efficiency. The role of m\n                    <jats:sup>6<\/jats:sup>\n                    A in both experimental and human epilepsy remains unknown. Here, we used transcriptome-wide m\n                    <jats:sup>6<\/jats:sup>\n                    A arrays to obtain a detailed analysis of the hippocampal m\n                    <jats:sup>6<\/jats:sup>\n                    A-ome from both mouse and human epilepsy samples. We combined this with human proteomic analyses and show that epileptic tissue displays disrupted metabolic and autophagic pathways that may be directly linked to m\n                    <jats:sup>6<\/jats:sup>\n                    A processing. Specifically, our results suggest that m\n                    <jats:sup>6<\/jats:sup>\n                    A levels inversely correlate with protein pathway activation. Finally, we show that elevated levels of m\n                    <jats:sup>6<\/jats:sup>\n                    A decrease seizure susceptibility and severity in mice. Together, our findings indicate that m\n                    <jats:sup>6<\/jats:sup>\n                    A represents an additional layer of gene regulation complexity in epilepsy and may contribute to the pathomechanisms that drive the development and maintenance of hyperexcitable brain networks.\n                  <\/jats:p>","DOI":"10.1172\/jci.insight.188612","type":"journal-article","created":{"date-parts":[[2025,7,21]],"date-time":"2025-07-21T18:00:27Z","timestamp":1753120827000},"source":"Crossref","is-referenced-by-count":8,"title":["N6-methyladenosine (m6A) dysregulation contributes to network excitability in temporal lobe epilepsy"],"prefix":"10.1172","volume":"10","author":[{"given":"Justine","family":"Mathoux","sequence":"first","affiliation":[{"name":"Department of Physiology and Medical Physics, RCSI University of Medicine and Health Sciences, Dublin, Ireland."},{"name":"FutureNeuro Research Ireland Centre for Translational Brain Science, and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marc-Michel","family":"Wilson","sequence":"additional","affiliation":[{"name":"Department of Physiology and Medical Physics, RCSI University of Medicine and Health Sciences, Dublin, Ireland."},{"name":"FutureNeuro Research Ireland Centre for Translational Brain Science, and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sujithra","family":"Srinivas","sequence":"additional","affiliation":[{"name":"FutureNeuro Research Ireland Centre for Translational Brain Science, and"},{"name":"UCD School of Biomolecular and Biomedical Science, University College Dublin, Belfield, Dublin 4, Ireland."},{"name":"UCD Conway Institute, University College Dublin, Dublin, Ireland."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gabrielle","family":"Litovskich","sequence":"additional","affiliation":[{"name":"UCD School of Biomolecular and Biomedical Science, University College Dublin, Belfield, Dublin 4, Ireland."},{"name":"UCD Conway Institute, University College Dublin, Dublin, Ireland."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leticia","family":"Villalba Benito","sequence":"additional","affiliation":[{"name":"UCD School of Biomolecular and Biomedical Science, University College Dublin, Belfield, Dublin 4, Ireland."},{"name":"UCD Conway Institute, University College Dublin, Dublin, Ireland."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cindy","family":"Tran","sequence":"additional","affiliation":[{"name":"Department of Neuroscience, Central Clinical School, Monash University, Melbourne, Victoria, Australia."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jaideep","family":"Kesavan","sequence":"additional","affiliation":[{"name":"Department of Physiology and Medical Physics, RCSI University of Medicine and Health Sciences, Dublin, Ireland."},{"name":"FutureNeuro Research Ireland Centre for Translational Brain Science, and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aileen","family":"Harnett","sequence":"additional","affiliation":[{"name":"Department of Physiology and Medical Physics, RCSI University of Medicine and Health Sciences, Dublin, Ireland."},{"name":"FutureNeuro Research Ireland Centre for Translational Brain Science, and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Theresa","family":"Auer","sequence":"additional","affiliation":[{"name":"UCD School of Biomolecular and Biomedical Science, University College Dublin, Belfield, Dublin 4, Ireland."},{"name":"UCD Conway Institute, University College Dublin, Dublin, Ireland."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amaya","family":"Sanz-Rodriguez","sequence":"additional","affiliation":[{"name":"Department of Physiology and Medical Physics, RCSI University of Medicine and Health Sciences, Dublin, Ireland."},{"name":"FutureNeuro Research Ireland Centre for Translational Brain Science, and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohammad","family":"Kh. A.E. Alkhayyat","sequence":"additional","affiliation":[{"name":"UCD School of Biomolecular and Biomedical Science, University College Dublin, Belfield, Dublin 4, Ireland."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mair\u00e9ad","family":"Sullivan","sequence":"additional","affiliation":[{"name":"UCD Conway Institute, University College Dublin, Dublin, Ireland."},{"name":"UCD School of Medicine, University College Dublin, Belfield, Dublin, Ireland."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zining","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Neuroscience, Central Clinical School, Monash University, Melbourne, Victoria, Australia."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yifan","family":"Huang","sequence":"additional","affiliation":[{"name":"Department of Neuroscience, Central Clinical School, Monash University, Melbourne, Victoria, Australia."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Austin","family":"Lacey","sequence":"additional","affiliation":[{"name":"FutureNeuro Research Ireland Centre for Translational Brain Science, and"},{"name":"School of Pharmacy and Biomolecular Sciences, RCSI University of Medicine and Health Sciences, Dublin, Ireland."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Norman","family":"Delanty","sequence":"additional","affiliation":[{"name":"FutureNeuro Research Ireland Centre for Translational Brain Science, and"},{"name":"School of Pharmacy and Biomolecular Sciences, RCSI University of Medicine and Health Sciences, Dublin, Ireland."},{"name":"Department of Neurology,"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jane","family":"Cryan","sequence":"additional","affiliation":[{"name":"Department of Neuropathology, and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francesca M.","family":"Brett","sequence":"additional","affiliation":[{"name":"Department of Neuropathology, and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael A.","family":"Farrell","sequence":"additional","affiliation":[{"name":"Department of Neuropathology, and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Donncha F.","family":"O\u2019Brien","sequence":"additional","affiliation":[{"name":"Department of Neurosurgery, Beaumont Hospital Dublin, Dublin, Ireland."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pablo M.","family":"Casillas-Espinosa","sequence":"additional","affiliation":[{"name":"Department of Neuroscience, Central Clinical School, Monash University, Melbourne, Victoria, Australia."},{"name":"Department of Neurology, The Alfred Hospital, Commercial Road, Melbourne, Victoria, Australia."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eva M.","family":"Jimenez-Mateos","sequence":"additional","affiliation":[{"name":"Discipline of Physiology, School of Medicine, Trinity College Dublin, The University of Dublin, Dublin, Ireland."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeffrey C.","family":"Glennon","sequence":"additional","affiliation":[{"name":"UCD Conway Institute, University College Dublin, Dublin, Ireland."},{"name":"UCD School of Medicine, University College Dublin, Belfield, Dublin, Ireland."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2310-2563","authenticated-orcid":true,"given":"Mary","family":"Canavan","sequence":"additional","affiliation":[{"name":"Translational Immunopathology, School of Biochemistry and Immunology and School of Medicine, Trinity College Dublin, Dublin, Ireland."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6237-9632","authenticated-orcid":true,"given":"David C.","family":"Henshall","sequence":"additional","affiliation":[{"name":"Department of Physiology and Medical Physics, RCSI University of Medicine and Health Sciences, Dublin, Ireland."},{"name":"FutureNeuro Research Ireland Centre for Translational Brain Science, and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gary P.","family":"Brennan","sequence":"additional","affiliation":[{"name":"FutureNeuro Research Ireland Centre for Translational Brain Science, and"},{"name":"UCD School of Biomolecular and Biomedical Science, University College Dublin, Belfield, Dublin 4, Ireland."},{"name":"UCD Conway Institute, University College Dublin, Dublin, Ireland."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"232","published-online":{"date-parts":[[2025,7,22]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","DOI":"10.1038\/s41582-024-00954-y"},{"key":"B2","doi-asserted-by":"publisher","DOI":"10.1111\/epi.13965"},{"key":"B3","doi-asserted-by":"publisher","DOI":"10.1684\/epd.2020.1159"},{"key":"B4","doi-asserted-by":"publisher","DOI":"10.1016\/S1474-4422(15)00248-3"},{"key":"B5","doi-asserted-by":"publisher","DOI":"10.1101\/cshperspect.a022822"},{"key":"B6","doi-asserted-by":"publisher","DOI":"10.1002\/epi4.12851"},{"key":"B7","doi-asserted-by":"publisher","DOI":"10.1038\/s41582-022-00693-y"},{"key":"B8","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.01267"},{"key":"B9","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-020-18752-7"},{"key":"B10","doi-asserted-by":"publisher","DOI":"10.1038\/s41582-020-0369-8"},{"key":"B11","doi-asserted-by":"publisher","DOI":"10.1007\/s00401-024-02683-4"},{"key":"B12","doi-asserted-by":"publisher","DOI":"10.1038\/s41583-019-0244-z"},{"key":"B13","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-024-47953-7"},{"key":"B14","doi-asserted-by":"publisher","DOI":"10.1038\/s41380-021-01282-z"},{"key":"B15","doi-asserted-by":"publisher","DOI":"10.1038\/s41593-018-0173-6"},{"key":"B16","doi-asserted-by":"publisher","DOI":"10.1038\/s41380-023-02083-2"},{"key":"B17","doi-asserted-by":"publisher","DOI":"10.1038\/nature11112"},{"key":"B18","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2012.05.003"},{"key":"B19","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2019.06.072"},{"key":"B20","doi-asserted-by":"publisher","DOI":"10.1038\/nature12730"},{"key":"B21","doi-asserted-by":"publisher","DOI":"10.1016\/1357-2725(96)00014-3"},{"key":"B22","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2015.05.014"},{"key":"B23","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/2.7.1043"},{"key":"B24","doi-asserted-by":"publisher","DOI":"10.3389\/fncel.2021.671932"},{"key":"B25","doi-asserted-by":"publisher","DOI":"10.15252\/embj.2020105977"},{"key":"B26","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbagrm.2018.10.014"},{"key":"B27","doi-asserted-by":"publisher","DOI":"10.1261\/rna.079988.124"},{"key":"B28","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2017.09.003"},{"key":"B29","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.4053-15.2016"},{"key":"B30","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2018.07.009"},{"key":"B31","doi-asserted-by":"publisher","DOI":"10.1038\/s41380-024-02574-w"},{"key":"B32","doi-asserted-by":"publisher","DOI":"10.4103\/1673-5374.385858"},{"key":"B33","doi-asserted-by":"publisher","DOI":"10.1016\/j.neulet.2020.135315"},{"key":"B34","doi-asserted-by":"publisher","DOI":"10.1021\/acschemneuro.8b00657"},{"key":"B35","doi-asserted-by":"publisher","DOI":"10.2217\/epi-2019-0002"},{"key":"B36","doi-asserted-by":"publisher","DOI":"10.1038\/nature04689"},{"key":"B37","doi-asserted-by":"publisher","DOI":"10.1016\/j.ccell.2019.03.006"},{"key":"B38","doi-asserted-by":"publisher","DOI":"10.1002\/syn.22270"},{"key":"B39","doi-asserted-by":"publisher","DOI":"10.3389\/fgene.2022.1042543"},{"key":"B40","doi-asserted-by":"publisher","DOI":"10.7150\/thno.100703"},{"key":"B41","doi-asserted-by":"publisher","DOI":"10.1002\/advs.202105731"},{"key":"B42","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.79994"},{"key":"B43","doi-asserted-by":"publisher","DOI":"10.1038\/s41419-021-03992-2"},{"key":"B44","doi-asserted-by":"publisher","DOI":"10.1038\/s41593-024-01850-w"},{"key":"B45","doi-asserted-by":"publisher","DOI":"10.1096\/fj.202400664R"},{"key":"B46","doi-asserted-by":"publisher","DOI":"10.1016\/j.nlm.2024.107903"},{"key":"B47","doi-asserted-by":"publisher","DOI":"10.1002\/1873-3468.12621"},{"key":"B48","doi-asserted-by":"publisher","DOI":"10.1186\/s13024-021-00484-x"},{"key":"B49","doi-asserted-by":"publisher","DOI":"10.1007\/s00401-022-02511-7"},{"key":"B50","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2022.12.019"},{"key":"B51","doi-asserted-by":"publisher","DOI":"10.1161\/STROKEAHA.122.040401"},{"key":"B52","doi-asserted-by":"publisher","DOI":"10.1161\/STROKEAHA.119.026433"},{"key":"B53","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/ddae029"},{"key":"B54","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.87283.3"},{"key":"B55","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2216658120"},{"key":"B56","doi-asserted-by":"publisher","DOI":"10.1016\/j.nbd.2019.104612"},{"key":"B57","doi-asserted-by":"publisher","DOI":"10.1101\/gr.278424.123"},{"key":"B58","doi-asserted-by":"publisher","DOI":"10.1038\/nm.2834"},{"key":"B59","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.21-13-04789.2001"},{"key":"B60","doi-asserted-by":"publisher","DOI":"10.1038\/nbt.1511"},{"key":"B61","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2016.136"},{"key":"B62","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkae1011"}],"container-title":["JCI Insight"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/insight.jci.org\/articles\/view\/188612\/files\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,18]],"date-time":"2026-05-18T16:42:00Z","timestamp":1779122520000},"score":1,"resource":{"primary":{"URL":"https:\/\/insight.jci.org\/articles\/view\/188612"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,7,22]]},"references-count":62,"journal-issue":{"issue":"14","published-online":{"date-parts":[[2025,6,12]]},"published-print":{"date-parts":[[2025,7,22]]}},"alternative-id":["10.1172\/jci.insight.188612"],"URL":"https:\/\/doi.org\/10.1172\/jci.insight.188612","relation":{},"ISSN":["2379-3708"],"issn-type":[{"value":"2379-3708","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,7,22]]},"article-number":"e188612"}}