{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,2]],"date-time":"2026-11-02T19:34:23Z","timestamp":1793648063236,"version":"4.5.1"},"reference-count":60,"publisher":"American Society for Clinical Investigation","issue":"15","license":[{"start":{"date-parts":[[2025,12,8]],"date-time":"2025-12-08T00:00:00Z","timestamp":1765152000000},"content-version":"vor","delay-in-days":165,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Specialized Program of Research Excellence (SPORE) in Gastrointestinal Cancer at the Sidney Kimmel Comprehensive Cancer Center at Johns Hopkins","award":["P50CA062924"],"award-info":[{"award-number":["P50CA062924"]}],"id":[{"id":"https:\/\/ror.org\/05m5b8x20","id-type":"ROR","asserted-by":"crossref"}]},{"DOI":"10.13039\/100000069","name":"National Institute of Arthritis and Musculoskeletal and Skin Diseases","doi-asserted-by":"crossref","award":["U54AR081774"],"award-info":[{"award-number":["U54AR081774"]}],"id":[{"id":"10.13039\/100000069","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100005979","name":"Lustgarten Foundation","doi-asserted-by":"crossref","award":["A Translational Convergence Program of Personalized Immunotherapy for Pancreatic Cancer Patients at Johns Hopkins"],"award-info":[{"award-number":["A Translational Convergence Program of Personalized Immunotherapy for Pancreatic Cancer Patients at Johns Hopkins"]}],"id":[{"id":"10.13039\/100005979","id-type":"DOI","asserted-by":"crossref"}]},{"name":"National Cancer Institute","award":["R01CA197296,U54CA143868,U54CA268083,F31CA284525,P01CA247886,K08CA248624"],"award-info":[{"award-number":["R01CA197296,U54CA143868,U54CA268083,F31CA284525,P01CA247886,K08CA248624"]}]},{"name":"National Cancer Institute","award":["R37CA292056"],"award-info":[{"award-number":["R37CA292056"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,8,8]]},"abstract":"<jats:p>\n                    Pancreatic ductal adenocarcinoma (PDAC) has a poor survival rate due to late detection. PDAC arises from precursor microscopic lesions, termed pancreatic intraepithelial neoplasia (PanIN), that develop at least a decade before overt disease; this provides an opportunity to intercept PanIN-to-PDAC progression. However, immune interception strategies require full understanding of PanIN and PDAC cellular architecture. Surgical specimens containing PanIN and PDAC lesions from a unique cohort of 5 treatment-naive patients with PDAC were surveyed using spatial omics (proteomic and transcriptomic). Findings were corroborated by spatial proteomics of PanIN and PDAC from tamoxifen-inducible KPC mice. We uncovered the organization of lymphoid cells into tertiary lymphoid structures (TLSs) adjacent to PanIN lesions. These TLSs lacked CD21\n                    <jats:sup>+<\/jats:sup>\n                    CD23\n                    <jats:sup>+<\/jats:sup>\n                    B cells compared with more mature TLSs near the PDAC border. PanINs harbored mostly CD4\n                    <jats:sup>+<\/jats:sup>\n                    T cells, with fewer Tregs and exhausted T cells than PDAC. Peritumoral space was enriched with naive CD4\n                    <jats:sup>+<\/jats:sup>\n                    and central memory T cells. These observations highlight the opportunity to modulate the immune microenvironment in PanINs before immune exclusion and immunosuppression emerge during progression into PDAC.\n                  <\/jats:p>","DOI":"10.1172\/jci.insight.191595","type":"journal-article","created":{"date-parts":[[2025,6,26]],"date-time":"2025-06-26T12:00:14Z","timestamp":1750939214000},"source":"Crossref","is-referenced-by-count":18,"title":["Spatial proteomics and transcriptomics reveal early immune cell organization in pancreatic intraepithelial neoplasia"],"prefix":"10.1172","volume":"10","author":[{"given":"Melissa R.","family":"Lyman","sequence":"first","affiliation":[{"name":"Department of Oncology, Sidney Kimmel Comprehensive Cancer Center,"},{"name":"Convergence Institute,"},{"name":"Bloomberg Kimmel Immunology Institute,"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5370-9692","authenticated-orcid":true,"given":"Jacob 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