{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,17]],"date-time":"2026-05-17T15:09:00Z","timestamp":1779030540038,"version":"3.51.4"},"reference-count":62,"publisher":"American Society for Clinical Investigation","issue":"19","license":[{"start":{"date-parts":[[2025,10,8]],"date-time":"2025-10-08T00:00:00Z","timestamp":1759881600000},"content-version":"vor","delay-in-days":36,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National institute of health","award":["AI171980"],"award-info":[{"award-number":["AI171980"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,10,8]]},"abstract":"<jats:p>\n                    Recent findings suggest that the small intestine (SI) is a potentially novel site for B cell lymphopoiesis during fetal and neonatal life. However, the unique and\/or conserved features that enable B cell development at this site remain unclear. To investigate the molecular and cellular scaffolds for B cell lymphopoiesis in mouse and human fetal intestines, we leveraged single-cell RNA-Seq, in situ immunofluorescence, spatial transcriptomics, and high-dimensional spectral flow cytometry. We found that SI mesenchymal and stromal cells expressed higher levels of chemokines known to recruit common lymphoid progenitors. Importantly, local lymphatic endothelial cells expressed IL-7 and TSLP in proximity to IL-7R\n                    <jats:sup>+<\/jats:sup>\n                    precursor B cells, likely promoting their differentiation in the SI. Notably, we found that fetal-derived lymphoid tissue inducer (LTi) cells were required for B cell development and localization in the SI, but not fetal liver. These findings identify a lymphoid tissue development\u2013independent role for this immune cell in B cell development. Collectively, our data reveal a conserved intestinal B cell niche in mice and humans, challenging traditional models of lymphopoiesis. The identification of a requisite cellular\/molecular scaffold for fetal B cell development allows future studies to test the importance of this de novo B cell lymphopoiesis to long-term immunity.\n                  <\/jats:p>","DOI":"10.1172\/jci.insight.192550","type":"journal-article","created":{"date-parts":[[2025,9,2]],"date-time":"2025-09-02T16:00:59Z","timestamp":1756828859000},"source":"Crossref","is-referenced-by-count":0,"title":["Conserved interactions with stromal and immune cells coordinate de novo B cell lymphopoiesis in fetal intestines"],"prefix":"10.1172","volume":"10","author":[{"given":"Kimberly A.","family":"Carroll","sequence":"first","affiliation":[{"name":"Graduate School of Biomedical Sciences, Tufts University, Boston, Massachusetts, USA."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Weihong","family":"Gu","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, Yale School of Medicine, New Haven, Connecticut, 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