{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T14:51:44Z","timestamp":1787064704038,"version":"build-2736575974"},"reference-count":33,"publisher":"American Society for Clinical Investigation","issue":"4","license":[{"start":{"date-parts":[[2026,3,25]],"date-time":"2026-03-25T00:00:00Z","timestamp":1774396800000},"content-version":"vor","delay-in-days":76,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"JSPS KAKENHI","award":["24K02437"],"award-info":[{"award-number":["24K02437"]}]},{"name":"Agency for Medical Research and Development","award":["23ek0109597"],"award-info":[{"award-number":["23ek0109597"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,2,23]]},"abstract":"<jats:p>\n                    Ulcerative colitis (UC) is a chronic inflammatory condition of the colon that primarily affects the mucosal layer. Previously, we identified autoantibodies against integrin \u03b1\n                    <jats:sub>v<\/jats:sub>\n                    \u03b2\n                    <jats:sub>6<\/jats:sub>\n                    in patients with UC. In this study, we established monoclonal antibodies (mAbs) from patients with UC to reveal the features and functions of these anti\u2013integrin \u03b1\n                    <jats:sub>v<\/jats:sub>\n                    \u03b2\n                    <jats:sub>6<\/jats:sub>\n                    autoantibodies. We identified two shared heavy chain complementarity-determining region 3 (CDR3) amino acid sequences among different patients with UC. Notably, several mAbs contained the RGD sequence in their heavy chain CDR3 that mimicked the key recognition sequence of integrin \u03b1\n                    <jats:sub>v<\/jats:sub>\n                    \u03b2\n                    <jats:sub>6<\/jats:sub>\n                    ligands such as fibronectin. Almost all mAbs selectively reacted with integrin \u03b1\n                    <jats:sub>v<\/jats:sub>\n                    \u03b2\n                    <jats:sub>6<\/jats:sub>\n                    in the presence of divalent cations (Ca\n                    <jats:sup>2+<\/jats:sup>\n                    and Mg\n                    <jats:sup>2+<\/jats:sup>\n                    ) and blocked fibronectin\u2013integrin \u03b1\n                    <jats:sub>v<\/jats:sub>\n                    \u03b2\n                    <jats:sub>6<\/jats:sub>\n                    binding. MAbs that shared the same heavy chain CDR3 amino acid sequence showed differences in reactivity to integrin \u03b1\n                    <jats:sub>v<\/jats:sub>\n                    \u03b2\n                    <jats:sub>6<\/jats:sub>\n                    , indicating that the reactivity of these mAbs is also affected by the light chain. Some of the mAbs showed varying degrees of cross-reactivity with integrin \u03b1\n                    <jats:sub>v<\/jats:sub>\n                    \u03b2\n                    <jats:sub>3<\/jats:sub>\n                    . The identification of shared CDR3 amino acid sequences in anti\u2013integrin \u03b1\n                    <jats:sub>v<\/jats:sub>\n                    \u03b2\n                    <jats:sub>6<\/jats:sub>\n                    antibodies from several patients with UC suggests a common mechanism underlying their production, which may help elucidate the pathogenesis of UC.\n                  <\/jats:p>","DOI":"10.1172\/jci.insight.192953","type":"journal-article","created":{"date-parts":[[2026,1,8]],"date-time":"2026-01-08T18:39:33Z","timestamp":1767897573000},"source":"Crossref","is-referenced-by-count":2,"title":["Characteristics of anti\u2013integrin \u03b1v\u03b26 autoantibodies in patients with ulcerative colitis"],"prefix":"10.1172","volume":"11","author":[{"given":"Ikuhisa","family":"Takimoto","sequence":"first","affiliation":[{"name":"Department of Gastroenterology and Hepatology, Kyoto University Graduate School of Medicine, Sakyo-ku, Kyoto, Japan."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masahiro","family":"Shiokawa","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology and Hepatology, Kyoto University Graduate School of Medicine, Sakyo-ku, Kyoto, Japan."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1113-6937","authenticated-orcid":true,"given":"Yoshihiro","family":"Nishikawa","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology and Hepatology, Kyoto University Graduate School of Medicine, Sakyo-ku, Kyoto, Japan."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9808-4139","authenticated-orcid":true,"given":"Takeshi","family":"Kuwada","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology and Hepatology, Kyoto University Graduate School of Medicine, Sakyo-ku, Kyoto, 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