{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,15]],"date-time":"2026-05-15T20:10:50Z","timestamp":1778875850671,"version":"3.51.4"},"reference-count":55,"publisher":"American Society for Clinical Investigation","issue":"5","license":[{"start":{"date-parts":[[2026,3,9]],"date-time":"2026-03-09T00:00:00Z","timestamp":1773014400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"crossref","award":["R01HL164583"],"award-info":[{"award-number":["R01HL164583"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,3,9]]},"abstract":"<jats:p>\n                    Vaso-occlusive episodes (VOEs) or acute pain events, involving complex interactions between sickle erythrocytes and other blood cells, are a hallmark of sickle cell disease (SCD). In this study, we analyzed changes in peripheral blood transcriptomes between steady state and VOEs in individuals with SCD. We followed a cohort of 174 individuals with SCD with or without chronic pain and collected peripheral blood at clinic visits (steady state) and during hospitalizations (VOEs). We performed RNA-Seq profiling of CD45\n                    <jats:sup>+<\/jats:sup>\n                    leukocytes and CD71\n                    <jats:sup>+<\/jats:sup>\n                    erythroid cells. Pathways linked to complement activation, coagulation, and IL-6\/JAK\/STAT3 signaling were enriched during VOEs in the CD45\n                    <jats:sup>+<\/jats:sup>\n                    cells. Contrastingly, the CD71\n                    <jats:sup>+<\/jats:sup>\n                    cells showed an enrichment of pathways related to the cell cycle, such as mTORC1 signaling and the G\n                    <jats:sub>2<\/jats:sub>\n                    M checkpoint during VOEs. We then analyzed the expression changes of genes in patients with longitudinal data to determine potential biomarkers for VOEs. Expression of 4 genes \u2014\n                    <jats:italic>FAM20A<\/jats:italic>\n                    ,\n                    <jats:italic>IL1B<\/jats:italic>\n                    ,\n                    <jats:italic>MS4A4A<\/jats:italic>\n                    , and\n                    <jats:italic>SERPINB2<\/jats:italic>\n                    \u2014 was elevated during VOEs compared with steady state in the majority of patients. Furthermore, our results indicate that patients experiencing chronic pain exhibited 44% increased enrichment of significant pathways during VOEs when compared with patients without chronic pain.\n                  <\/jats:p>","DOI":"10.1172\/jci.insight.193359","type":"journal-article","created":{"date-parts":[[2026,3,8]],"date-time":"2026-03-08T18:00:41Z","timestamp":1772992841000},"source":"Crossref","is-referenced-by-count":0,"title":["Gene expression profiling identifies potential biomarkers for vaso-occlusive episodes in sickle cell disease"],"prefix":"10.1172","volume":"11","author":[{"given":"Varsha","family":"Bhat","sequence":"first","affiliation":[{"name":"Center for Integrative Genomics, School of Biological Sciences, Georgia Institute of Technology, Atlanta, Georgia, USA."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Justin J.","family":"Yoo","sequence":"additional","affiliation":[{"name":"Aflac Cancer and Blood Disorders Center, Children\u2019s Healthcare of Atlanta, Emory University School of Medicine, Atlanta, Georgia, USA."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Srija","family":"Ponna","sequence":"additional","affiliation":[{"name":"Aflac Cancer and Blood Disorders Center, Children\u2019s Healthcare of Atlanta, Emory University School of Medicine, Atlanta, Georgia, USA."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alka A.","family":"Potdar","sequence":"additional","affiliation":[{"name":"Pfizer, South San Francisco, California, USA."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ashwin P.","family":"Patel","sequence":"additional","affiliation":[{"name":"Aflac Cancer and Blood Disorders Center, Children\u2019s Healthcare of Atlanta, Emory University School of Medicine, Atlanta, Georgia, USA."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G. 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