{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,29]],"date-time":"2026-06-29T18:47:08Z","timestamp":1782758828903,"version":"3.54.5"},"reference-count":45,"publisher":"American Society for Clinical Investigation","issue":"11","license":[{"start":{"date-parts":[[2026,6,8]],"date-time":"2026-06-08T00:00:00Z","timestamp":1780876800000},"content-version":"vor","delay-in-days":46,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"NIH\/NIAID","award":["R01 AI057029,R01 AI071852,R01 AI074417"],"award-info":[{"award-number":["R01 AI057029,R01 AI071852,R01 AI074417"]}]},{"name":"Emory National Primate Research Center base grant","award":["P51 RR00165"],"award-info":[{"award-number":["P51 RR00165"]}]},{"name":"Emory CFAR grant","award":["P30 AI050409"],"award-info":[{"award-number":["P30 AI050409"]}]},{"name":"Office of Research Infrastructure Programs","award":["OD P51OD011132"],"award-info":[{"award-number":["OD P51OD011132"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,6,8]]},"abstract":"<jats:p>\n                    Inhibiting the mammalian target of rapamycin (mTOR) during acute viral infection generates highly functional memory CD8\n                    <jats:sup>+<\/jats:sup>\n                    T cells. We investigated the effects of inhibiting mTOR by using rapamycin during the effector and contraction phases of the immune response to a DNA prime and Modified Vaccinia Ankara (MVA) boost SIV vaccination in rhesus macaques. Rapamycin administered either during MVA boosts alone (DM\n                    <jats:sub>R<\/jats:sub>\n                    ) or during both primes and boosts (D\n                    <jats:sub>R<\/jats:sub>\n                    M\n                    <jats:sub>R<\/jats:sub>\n                    ) reduced the contraction of effector CD8\n                    <jats:sup>+<\/jats:sup>\n                    T cells, resulting in higher frequencies of SIV-specific memory CD8\n                    <jats:sup>+<\/jats:sup>\n                    T cells with enhanced quality, as indicated by expression of Bcl2 and CD127. Additionally, rapamycin reduced the frequency of proliferating CCR5\n                    <jats:sup>+<\/jats:sup>\n                    CD4\n                    <jats:sup>+<\/jats:sup>\n                    T cells in the blood following the MVA boost. After SIVmac251 infection, rapamycin-treated macaques demonstrated marked expansion of SIV-specific CD8\n                    <jats:sup>+<\/jats:sup>\n                    T cells (reaching up to 50% in blood and 25% in gut). The heightened expansion of SIV-specific CD8\n                    <jats:sup>+<\/jats:sup>\n                    T cells in the DM\n                    <jats:sub>R<\/jats:sub>\n                    group was associated with markedly lower (2-logs compared with unvaccinated and 1-log compared with DM) peak viral load in the gut and set-point viremia, along with improved survival after infection. Thus, inhibiting the mTOR pathway during MVA boosts of a DNA\/MVA vaccine enhances vaccine efficacy by improving memory CD4\n                    <jats:sup>+<\/jats:sup>\n                    and CD8\n                    <jats:sup>+<\/jats:sup>\n                    T cell function.\n                  <\/jats:p>","DOI":"10.1172\/jci.insight.193752","type":"journal-article","created":{"date-parts":[[2026,4,23]],"date-time":"2026-04-23T16:01:01Z","timestamp":1776960061000},"source":"Crossref","is-referenced-by-count":0,"title":["Coadministration of rapamycin with a DNA\/MVA SIV vaccine improves memory CD8+ T cell response"],"prefix":"10.1172","volume":"11","author":[{"given":"Shanmugalakshmi","family":"Sadagopal","sequence":"first","affiliation":[{"name":"Emory Vaccine Center, Emory National Primate Research Center, and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kasey","family":"Stokdyk","sequence":"additional","affiliation":[{"name":"Emory Vaccine Center, Emory 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