{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,2]],"date-time":"2026-06-02T07:22:13Z","timestamp":1780384933569,"version":"3.54.1"},"reference-count":47,"publisher":"American Society for Clinical Investigation","issue":"4","license":[{"start":{"date-parts":[[2026,2,23]],"date-time":"2026-02-23T00:00:00Z","timestamp":1771804800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Cancer Institute","award":["F30CA265281"],"award-info":[{"award-number":["F30CA265281"]}]},{"name":"National Cancer Institute","award":["UG1CA233338"],"award-info":[{"award-number":["UG1CA233338"]}]},{"DOI":"10.13039\/100015979","name":"Pelotonia","doi-asserted-by":"crossref","award":["Graduate Student Fellowship"],"award-info":[{"award-number":["Graduate Student Fellowship"]}],"id":[{"id":"10.13039\/100015979","id-type":"DOI","asserted-by":"crossref"}]},{"name":"U.S. Department of Defense","award":["HT9425-24-1-0821"],"award-info":[{"award-number":["HT9425-24-1-0821"]}]},{"name":"National Cancer Institute","award":["R35CA197734"],"award-info":[{"award-number":["R35CA197734"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,2,23]]},"abstract":"<jats:p>\n                    Mutations in protein tyrosine phosphatase non-receptor type 11 (\n                    <jats:italic>PTPN11<\/jats:italic>\n                    ) have been considered late acquired mutations in acute myeloid leukemia (AML) development. Using single-cell DNA sequencing, we found that\n                    <jats:italic>PTPN11<\/jats:italic>\n                    mutations can occur as initiating events in some patients with AML when accompanied by strong oncogenic drivers, commonly\n                    <jats:italic>NPM1<\/jats:italic>\n                    mutations. The resulting AML has a diverse set of variably differentiated myeloid cells with few myeloid cells that lack leukemic mutations. The role of\n                    <jats:italic>Ptpn11<\/jats:italic>\n                    as a codriver was confirmed in a murine model that exhibits an AML phenotype with a comparable immune diversity that is serially engraftable and reconstituted from early precursor cells. Furthermore, lineage-negative bone marrow cells from these mice reconstitute the full diversity of mature myeloid cells, and these cells exhibit an altered cytokine response after physiologic stimulation. Our work highlights how\n                    <jats:italic>PTPN11<\/jats:italic>\n                    -mutated AML is derived from a multitude of codominant and late acquired aberrations that have a previously unrecognized differentiated myeloid clonal expansion potentially contributing to pathogenesis of the disease.\n                  <\/jats:p>","DOI":"10.1172\/jci.insight.193779","type":"journal-article","created":{"date-parts":[[2026,2,23]],"date-time":"2026-02-23T16:20:29Z","timestamp":1771863629000},"source":"Crossref","is-referenced-by-count":1,"title":["Characterization of the clonal hierarchy and immunophenotype of PTPN11 mutations in acute myeloid leukemia"],"prefix":"10.1172","volume":"11","author":[{"given":"Sydney","family":"Fobare","sequence":"first","affiliation":[{"name":"Medical Scientist Training Program, The Ohio State University, Columbus, Ohio, USA."},{"name":"Department of Internal Medicine, University of Cincinnati, Cincinnati, Ohio, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chia","family":"Sharpe","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, University of Cincinnati, Cincinnati, Ohio, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kate","family":"Quinn","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, University of Cincinnati, Cincinnati, Ohio, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kinsey","family":"Bryant","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, University of Cincinnati, Cincinnati, Ohio, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Linde A.","family":"Miles","sequence":"additional","affiliation":[{"name":"Division of Experimental Hematology and Cancer Biology, Cincinnati Children\u2019s Hospital, Cincinnati, Ohio, USA."},{"name":"Department of Pediatrics, Cincinnati Children\u2019s Hospital Medical Center, Cincinnati, Ohio, USA."},{"name":"Cancer and Blood Diseases Institute, Cincinnati Children\u2019s Hospital, Cincinnati, Ohio, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert L.","family":"Bowman","sequence":"additional","affiliation":[{"name":"Department of Cancer Biology, University of Pennsylvania, Philadelphia, Pennsylvania, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carolyn","family":"Cheney","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, University of Cincinnati, Cincinnati, Ohio, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Casie","family":"Furby","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, University of Cincinnati, Cincinnati, Ohio, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marissa","family":"Long","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, University of Cincinnati, Cincinnati, Ohio, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kaytlynn","family":"Fyock","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, University of Cincinnati, Cincinnati, Ohio, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ben","family":"Wronowski","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, University of Cincinnati, Cincinnati, Ohio, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"James R.","family":"Lerma","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, University of Cincinnati, Cincinnati, Ohio, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1408-5063","authenticated-orcid":true,"given":"Krzysztof","family":"Mr\u00f3zek","sequence":"additional","affiliation":[{"name":"Clara D. 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