{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,1]],"date-time":"2026-11-01T11:56:22Z","timestamp":1793534182100,"version":"4.5.0"},"reference-count":71,"publisher":"American Society for Clinical Investigation","issue":"20","license":[{"start":{"date-parts":[[2025,9,23]],"date-time":"2025-09-23T00:00:00Z","timestamp":1758585600000},"content-version":"vor","delay-in-days":49,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"NIH","award":["R01AI114543"],"award-info":[{"award-number":["R01AI114543"]}]},{"name":"NIH","award":["R01AI042767"],"award-info":[{"award-number":["R01AI042767"]}]},{"name":"NIH","award":["GM140881"],"award-info":[{"award-number":["GM140881"]}]},{"name":"Veterans Administration Merit Review Award","award":["BX001324"],"award-info":[{"award-number":["BX001324"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,9,23]]},"abstract":"<jats:p>\n                    Radiation-induced lymphopenia (RIL) remains a challenging side effect of radiation therapy that is often associated with poor prognosis and reduced overall survival. Although CD8\n                    <jats:sup>+<\/jats:sup>\n                    T cells are highly radiosensitive, the dynamics of quantitative and qualitative changes to the CD8\n                    <jats:sup>+<\/jats:sup>\n                    T cell pool following exposure to high doses of ionizing radiation (IR) remain understudied. Herein, we sought to determine the long-term impact of sublethal whole body irradiation (WBI) on the antigen-inexperienced (Ag-inexperienced) CD8\n                    <jats:sup>+<\/jats:sup>\n                    T cell pool, comprising naive (T\n                    <jats:sub>N<\/jats:sub>\n                    ) and virtual memory (T\n                    <jats:sub>VM<\/jats:sub>\n                    ) CD8\n                    <jats:sup>+<\/jats:sup>\n                    T cells. We show that although both T\n                    <jats:sub>N<\/jats:sub>\n                    and T\n                    <jats:sub>VM<\/jats:sub>\n                    cells gradually regenerated after WBI-induced loss, T\n                    <jats:sub>N<\/jats:sub>\n                    recovery occurred only through de novo thymic production. Despite the numerical restoration, the subset and phenotypic composition of postrecovery Ag-inexperienced CD8\n                    <jats:sup>+<\/jats:sup>\n                    T cells did not qualitatively recapitulate the pre-WBI state. Specifically, the frequency of T\n                    <jats:sub>VM<\/jats:sub>\n                    cells is increased, especially during the early stages of recovery. Within the T\n                    <jats:sub>N<\/jats:sub>\n                    subset, a lasting overrepresentation of Ly6C\n                    <jats:sup>+<\/jats:sup>\n                    CD122\n                    <jats:sup>+<\/jats:sup>\n                    cells and an altered TCR clonotype diversity are also observed. Overall, our data highlight the dynamic changes to the Ag-inexperienced CD8\n                    <jats:sup>+<\/jats:sup>\n                    T cell pool upon recovery from RIL\n                  <\/jats:p>","DOI":"10.1172\/jci.insight.194201","type":"journal-article","created":{"date-parts":[[2025,8,5]],"date-time":"2025-08-05T16:00:12Z","timestamp":1754409612000},"source":"Crossref","is-referenced-by-count":5,"title":["Delineating the short- and long-term impact of ionizing radiation on antigen-inexperienced CD8+ T cell subsets"],"prefix":"10.1172","volume":"10","author":[{"given":"Mohammad","family":"Heidarian","sequence":"first","affiliation":[{"name":"Department of Pathology,"},{"name":"Experimental Pathology Graduate Program, and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shravan K.","family":"Kannan","sequence":"additional","affiliation":[{"name":"Department of Pathology,"},{"name":"Interdisciplinary Graduate Program in Immunology, University of Iowa, Iowa 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