{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T20:32:23Z","timestamp":1790627543651,"version":"4.1.0"},"reference-count":71,"publisher":"American Society for Clinical Investigation","issue":"5","license":[{"start":{"date-parts":[[2026,3,9]],"date-time":"2026-03-09T00:00:00Z","timestamp":1773014400000},"content-version":"vor","delay-in-days":46,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["U21A20348"],"award-info":[{"award-number":["U21A20348"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"name":"National Natural Science Young Scientists Foundation of China","award":["82103916,82200798"],"award-info":[{"award-number":["82103916,82200798"]}]},{"name":"Natural Science Foundation of Henan Province Excellent Young Scientists Fund Program","award":["202300410363"],"award-info":[{"award-number":["202300410363"]}]},{"name":"Henan Province Medical Science and Technology Joint Project","award":["LHGJ20250295"],"award-info":[{"award-number":["LHGJ20250295"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,3,9]]},"abstract":"<jats:p>\n                    Type 2 diabetic nephropathy (T\n                    <jats:sub>2<\/jats:sub>\n                    DN) is a major complication of type 2 diabetes and a leading cause of chronic kidney disease. This study aimed to explore Myosin IC (MYO1C) as both a candidate biomarker and elucidate its role as a mechanistic mediator of podocyte injury in T\n                    <jats:sub>2<\/jats:sub>\n                    DN. Using urinary extracellular vesicle RNA biomarkers identified from a training and validation cohort of 33 type 2 diabetes and 40 patients with T\n                    <jats:sub>2<\/jats:sub>\n                    DN, we developed a machine learning diagnostic model for T\n                    <jats:sub>2<\/jats:sub>\n                    DN. The model achieved an AUC of 0.877 in validation and performed well in an independent test cohort with an AUC of 0.824. MYO1C was identified as the most influential feature in the final model. Mechanistic investigations in vitro and in vivo revealed that high glucose and high-fat conditions induced podocyte injury, inflammation, and apoptosis, with increased MYO1C expression. MYO1C knockdown in vitro and in vivo reduced podocyte damage and inflammatory responses. MYO1C overexpression enhanced p38, p-CREB, and TNF-\u03b1 levels, while p38 inhibition mitigated these effects. These findings support MYO1C not only as a potential urinary biomarker for T\n                    <jats:sub>2<\/jats:sub>\n                    DN but also as a key pathogenic driver that promotes podocyte injury via p38 MAPK signaling.\n                  <\/jats:p>","DOI":"10.1172\/jci.insight.194604","type":"journal-article","created":{"date-parts":[[2026,1,22]],"date-time":"2026-01-22T17:00:17Z","timestamp":1769101217000},"source":"Crossref","is-referenced-by-count":2,"title":["MYO1C is a urinary extracellular vesicle biomarker and mediator of podocyte injury in diabetic nephropathy"],"prefix":"10.1172","volume":"11","author":[{"given":"Zihao","family":"Zhao","sequence":"first","affiliation":[{"name":"Department of Radiology, Department of Nephrology, and"},{"name":"Nephrology Research Center, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, China."},{"name":"Key Laboratory of Precision Diagnosis and Treatment for Chronic Kidney Disease in Henan Province, Zhengzhou, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qianqian","family":"Yan","sequence":"additional","affiliation":[{"name":"Department of Nephrology, Institute of Kidney Diseases, West China Hospital of Sichuan University, Chengdu, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sijie","family":"Zhou","sequence":"additional","affiliation":[{"name":"Nephrology Research Center, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, China."},{"name":"Key Laboratory of Precision Diagnosis and Treatment for Chronic Kidney Disease in Henan Province, Zhengzhou, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fengxun","family":"Liu","sequence":"additional","affiliation":[{"name":"Nephrology Research Center, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, China."},{"name":"Key Laboratory of Precision Diagnosis and Treatment for Chronic Kidney Disease in Henan Province, Zhengzhou, 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