{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T18:45:33Z","timestamp":1790189133800,"version":"4.1.0"},"reference-count":36,"publisher":"American Society for Clinical Investigation","issue":"20","license":[{"start":{"date-parts":[[2025,9,23]],"date-time":"2025-09-23T00:00:00Z","timestamp":1758585600000},"content-version":"vor","delay-in-days":42,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"NIH NIDDK","award":["U01DK133092,U01DK133093,U01DK133095,U01DK133097,U01DK114866,U01DK114908,U01DK133090,U01DK133113,U01DK133766,U01DK133768,U01DK114907,U01DK114920,U01DK114923,U01DK114933,U24DK114886,UH3DK114926,UH3DK114861,UH3DK114915,UH3DK114937,U01DK133081,U01DK133091"],"award-info":[{"award-number":["U01DK133092,U01DK133093,U01DK133095,U01DK133097,U01DK114866,U01DK114908,U01DK133090,U01DK133113,U01DK133766,U01DK133768,U01DK114907,U01DK114920,U01DK114923,U01DK114933,U24DK114886,UH3DK114926,UH3DK114861,UH3DK114915,UH3DK114937,U01DK133081,U01DK133091"]}]},{"name":"NIH NIDDK","award":["K23DK128358"],"award-info":[{"award-number":["K23DK128358"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,9,23]]},"abstract":"<jats:p>BACKGROUND. Urine proteomics may provide mechanistic insights on why patients experience a higher risk of kidney function decline after hospitalization. METHODS. In 174 patients with and without acute kidney injury (AKI) from the Assessment, Serial Evaluation, and Subsequent Sequelae in AKI (ASSESS-AKI) cohort, we used Olink to profile 2783 urinary proteins collected at 3 months after hospitalization and determined their association with estimated glomerular filtration rate (eGFR) decline during median [IQR] of 5.1 [4.0 to 6.0] years follow-up. In 4 independent cohorts, including the Kidney Precision Medicine Project (KPMP), we determined whether proteins were differentially expressed with AKI. We used weighted correlation network analysis to determine proteins\u2019 cellular enrichment in the kidney transcriptome (single-cell and spatial transcriptomics) in patients with AKI receiving research kidney biopsy.RESULTS. We identified 387 and 10 proteins associated with faster and slower eGFR decline, respectively, most of which were differentially expressed in patients at the time of AKI. Among these proteins, 283 (71%) were expressed by kidney cells in participants with AKI from KPMP. The expression formed 3 clusters enriched in the proximal tubule, degenerative tubule and myeloid cells, and stromal cells, and correlated with histopathological features of AKI, such as tubular injury, interstitial inflammation, and fibrosis, respectively.CONCLUSION. Urinary proteins reflecting degenerative tubular injury, inflammation, and fibrosis are associated with eGFR decline in recently hospitalized patients.FUNDING. National Institute of Diabetes and Digestive Kidney Diseases grants U01DK133081, U01DK133091, U01DK133092, U01DK133093, U01DK133095, U01DK133097, U01DK114866, U01DK114908, U01DK133090, U01DK133113, U01DK133766, U01DK133768, U01DK114907, U01DK114920, U01DK114923, U01DK114933, U24DK114886, UH3DK114926, UH3DK114861, UH3DK114915, UH3DK114937, K23DK128358, R01DK128087, and R01DK140717.<\/jats:p>","DOI":"10.1172\/jci.insight.195577","type":"journal-article","created":{"date-parts":[[2025,8,12]],"date-time":"2025-08-12T16:00:20Z","timestamp":1755014420000},"source":"Crossref","is-referenced-by-count":4,"title":["Urine proteomic signatures of kidney function decline after hospitalization"],"prefix":"10.1172","volume":"10","author":[{"given":"Yumeng","family":"Wen","sequence":"first","affiliation":[{"name":"Division of Nephrology, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2490-025X","authenticated-orcid":true,"given":"Steven","family":"Menez","sequence":"additional","affiliation":[{"name":"Division of Nephrology, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Heather","family":"Thiessen Philbrook","sequence":"additional","affiliation":[{"name":"Division of Nephrology, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9537-9038","authenticated-orcid":true,"given":"Dennis","family":"Moledina","sequence":"additional","affiliation":[{"name":"Section of Nephrology, Department of Medicine, Yale School of Medicine, New Haven, Connecticut, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0928-9168","authenticated-orcid":true,"given":"Steven G.","family":"Coca","sequence":"additional","affiliation":[{"name":"Division of Nephrology, Department of Medicine, Icahn School of Medicine at Mount Sinai, New York, New York, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiashu","family":"Xue","sequence":"additional","affiliation":[{"name":"Division of Nephrology, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6778-4167","authenticated-orcid":true,"given":"James","family":"Kaufman","sequence":"additional","affiliation":[{"name":"Division of Nephrology, New York University Grossman School of Medicine and VA New York Harbor Healthcare System, New York, New York, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vernon","family":"Chinchillil","sequence":"additional","affiliation":[{"name":"Department of Statistics, Pennsylvania State University College of Medicine, Hershey, Pennsylvania, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paul L.","family":"Kimmel","sequence":"additional","affiliation":[{"name":"Department of Medicine, George Washington University, Washington, DC, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5717-4218","authenticated-orcid":true,"given":"T. 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