{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T12:09:51Z","timestamp":1791288591865,"version":"4.3.0"},"reference-count":86,"publisher":"American Society for Clinical Investigation","issue":"6","license":[{"start":{"date-parts":[[2026,3,23]],"date-time":"2026-03-23T00:00:00Z","timestamp":1774224000000},"content-version":"vor","delay-in-days":41,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100010414","name":"Health Research Board","doi-asserted-by":"crossref","award":["EIA-2019-010"],"award-info":[{"award-number":["EIA-2019-010"]}],"id":[{"id":"10.13039\/100010414","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100010414","name":"Health Research Board","doi-asserted-by":"crossref","award":["ILP-POR-2024-044"],"award-info":[{"award-number":["ILP-POR-2024-044"]}],"id":[{"id":"10.13039\/100010414","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Royal City of Dublin Hospital Trust","award":["172b"],"award-info":[{"award-number":["172b"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,3,23]]},"abstract":"<jats:p>\n                    Infectious diseases remain a global health challenge, driven by increasing antimicrobial resistance and the threat of emerging epidemics.\n                    <jats:italic>Mycobacterium tuberculosis<\/jats:italic>\n                    and\n                    <jats:italic>Staphylococcus aureus<\/jats:italic>\n                    are leading causes of mortality worldwide. Trained immunity \u2014 a form of innate immune memory \u2014 offers a promising approach to enhance pathogen clearance. Here, we demonstrate that IFN-\u03b3 induces trained immunity in human monocytes through a mechanism involving mTORC1 activation, glutaminolysis, and epigenetic remodeling. Macrophages derived from IFN-\u03b3\u2013trained monocytes exhibited increased glycolytic activity with enhanced cytokine and chemokine responses upon stimulation or infection. Crucially, trained macrophages had increased production of reactive oxygen species, which mediated enhanced bactericidal activity against methicillin-resistant\n                    <jats:italic>S<\/jats:italic>\n                    .\n                    <jats:italic>aureus and M<\/jats:italic>\n                    .\n                    <jats:italic>tuberculosis<\/jats:italic>\n                    . Furthermore, ATAC-sequencing analysis of IFN-\u03b3\u2013trained macrophages revealed increased chromatin accessibility in regions associated with host defense. Last, IFN-\u03b3 training restored impaired innate responses in macrophages from individuals homozygous for the\n                    <jats:italic>TIRAP<\/jats:italic>\n                    180L polymorphism, a genetic variant associated with increased susceptibility to infection. These findings establish IFN-\u03b3 as a potent inducer of trained immunity in human monocytes and support its potential as a host-directed strategy to strengthen antimicrobial defenses, particularly in genetically susceptible individuals and high-risk clinical contexts.\n                  <\/jats:p>","DOI":"10.1172\/jci.insight.195866","type":"journal-article","created":{"date-parts":[[2026,2,10]],"date-time":"2026-02-10T17:00:52Z","timestamp":1770742852000},"source":"Crossref","is-referenced-by-count":3,"title":["IFN-\u03b3\u2013induced trained immunity enhances killing of priority pathogens in healthy and genetically vulnerable individuals"],"prefix":"10.1172","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0051-627X","authenticated-orcid":true,"given":"Dearbhla M.","family":"Murphy","sequence":"first","affiliation":[{"name":"School of Medicine, Trinity Translational Medicine Institute, St. James\u2019s Hospital, Trinity College Dublin, University of Dublin, Dublin, Ireland."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Isabella","family":"Batten","sequence":"additional","affiliation":[{"name":"School of Medicine, Trinity Translational Medicine Institute, St. James\u2019s Hospital, Trinity College Dublin, University of Dublin, Dublin, Ireland."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aoife","family":"O\u2019Farrell","sequence":"additional","affiliation":[{"name":"Department of Bioengineering, School of Engineering and Applied Sciences, University of Pennsylvania, Philadelphia, Pennsylvania, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Simon R.","family":"Carlile","sequence":"additional","affiliation":[{"name":"School of Biochemistry and Immunology, Trinity Biomedical Sciences Institute, Trinity College Dublin, University of Dublin, Dublin, Ireland."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sinead A.","family":"O\u2019Rourke","sequence":"additional","affiliation":[{"name":"School of Biochemistry and Immunology, Trinity Biomedical Sciences Institute, Trinity College Dublin, University of Dublin, Dublin, Ireland."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chloe","family":"Court","sequence":"additional","affiliation":[{"name":"School of Medicine, Trinity Translational Medicine Institute, St. James\u2019s Hospital, Trinity College Dublin, University of Dublin, Dublin, Ireland."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brenda","family":"Morris","sequence":"additional","affiliation":[{"name":"School of Biochemistry and Immunology, Trinity Biomedical Sciences Institute, Trinity College Dublin, University of Dublin, Dublin, Ireland."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gina","family":"Leisching","sequence":"additional","affiliation":[{"name":"School of Medicine, Trinity Translational Medicine Institute, St. James\u2019s Hospital, Trinity College Dublin, University of Dublin, Dublin, Ireland."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gr\u00e1inne","family":"Jameson","sequence":"additional","affiliation":[{"name":"School of Medicine, Trinity Translational Medicine Institute, St. James\u2019s Hospital, Trinity College Dublin, University of Dublin, Dublin, Ireland."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8887-1481","authenticated-orcid":true,"given":"Sarah A.","family":"Connolly","sequence":"additional","affiliation":[{"name":"School of Medicine, Trinity Translational Medicine Institute, St. James\u2019s Hospital, Trinity College Dublin, University of Dublin, Dublin, Ireland."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1356-510X","authenticated-orcid":true,"given":"Adam H.","family":"Dyer","sequence":"additional","affiliation":[{"name":"School of Medicine, Trinity Translational Medicine Institute, St. James\u2019s Hospital, Trinity College Dublin, University of Dublin, Dublin, Ireland."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John P.","family":"McGrath","sequence":"additional","affiliation":[{"name":"School of Medicine, Trinity Translational Medicine Institute, St. James\u2019s Hospital, Trinity College Dublin, University of Dublin, Dublin, Ireland."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emma","family":"McNally","sequence":"additional","affiliation":[{"name":"School of Medicine, Trinity Translational Medicine Institute, St. James\u2019s Hospital, Trinity College Dublin, University of Dublin, Dublin, Ireland."},{"name":"National Tuberculosis Centre, Department of Respiratory Medicine, and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Olivia","family":"Sandby-Thomas","sequence":"additional","affiliation":[{"name":"School of Medicine, Trinity Translational Medicine Institute, St. James\u2019s Hospital, Trinity College Dublin, University of Dublin, Dublin, Ireland."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anjali","family":"Yennemadi","sequence":"additional","affiliation":[{"name":"School of Medicine, Trinity Translational Medicine Institute, St. James\u2019s Hospital, Trinity College Dublin, University of Dublin, Dublin, Ireland."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Conor M.","family":"Finlay","sequence":"additional","affiliation":[{"name":"School of Medicine, Trinity Translational Medicine Institute, St. James\u2019s Hospital, Trinity College Dublin, University of Dublin, Dublin, Ireland."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cl\u00edona N\u00ed","family":"Cheallaigh","sequence":"additional","affiliation":[{"name":"School of Medicine, Trinity Translational Medicine Institute, St. James\u2019s Hospital, Trinity College Dublin, University of Dublin, Dublin, Ireland."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean","family":"Dunne","sequence":"additional","affiliation":[{"name":"School of Medicine, Trinity Translational Medicine Institute, St. James\u2019s Hospital, Trinity College Dublin, University of Dublin, Dublin, Ireland."},{"name":"Department of Clinical Immunology, St. James\u2019s Hospital, Dublin, Ireland."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cilian \u00d3","family":"Maoldomhnaigh","sequence":"additional","affiliation":[{"name":"School of Medicine, Trinity Translational Medicine Institute, St. James\u2019s Hospital, Trinity College Dublin, University of Dublin, Dublin, Ireland."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laura E.","family":"Gleeson","sequence":"additional","affiliation":[{"name":"School of Medicine, Trinity Translational Medicine Institute, St. James\u2019s Hospital, Trinity College Dublin, University of Dublin, Dublin, Ireland."},{"name":"National Tuberculosis Centre, Department of Respiratory Medicine, and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aisling","family":"Dunne","sequence":"additional","affiliation":[{"name":"School of Biochemistry and Immunology, Trinity Biomedical Sciences Institute, Trinity College Dublin, University of Dublin, Dublin, Ireland."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nollaig","family":"Bourke","sequence":"additional","affiliation":[{"name":"School of Medicine, Trinity Translational Medicine Institute, St. James\u2019s Hospital, Trinity College Dublin, University of Dublin, Dublin, Ireland."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6233-6410","authenticated-orcid":true,"given":"Reinout","family":"van Crevel","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine and Radboud Community for Infectious Diseases, Radboud University Medical Centre, Nijmegen, Netherlands."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8523-3836","authenticated-orcid":true,"given":"Donal J.","family":"Cox","sequence":"additional","affiliation":[{"name":"School of Medicine, Trinity Translational Medicine Institute, St. James\u2019s Hospital, Trinity College Dublin, University of Dublin, Dublin, Ireland."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Niall","family":"Conlon","sequence":"additional","affiliation":[{"name":"School of Medicine, Trinity Translational Medicine Institute, St. James\u2019s Hospital, Trinity College Dublin, University of Dublin, Dublin, Ireland."},{"name":"Department of Clinical Immunology, St. James\u2019s Hospital, Dublin, Ireland."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arjun","family":"Raj","sequence":"additional","affiliation":[{"name":"Department of Bioengineering, School of Engineering and Applied Sciences, University of Pennsylvania, Philadelphia, Pennsylvania, USA."},{"name":"Department of Genetics, Perelman School of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rachel M.","family":"McLoughlin","sequence":"additional","affiliation":[{"name":"School of Biochemistry and Immunology, Trinity Biomedical Sciences Institute, Trinity College Dublin, University of Dublin, Dublin, Ireland."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joseph","family":"Keane","sequence":"additional","affiliation":[{"name":"School of Medicine, Trinity Translational Medicine Institute, St. James\u2019s Hospital, Trinity College Dublin, University of Dublin, Dublin, Ireland."},{"name":"National Tuberculosis Centre, Department of Respiratory Medicine, and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5616-6665","authenticated-orcid":true,"given":"Sharee A.","family":"Basdeo","sequence":"additional","affiliation":[{"name":"School of Medicine, Trinity Translational Medicine Institute, St. James\u2019s Hospital, Trinity College Dublin, University of Dublin, Dublin, Ireland."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"232","published-online":{"date-parts":[[2026,2,10]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chom.2012.06.006"},{"key":"B2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1202870109"},{"key":"B3","doi-asserted-by":"publisher","DOI":"10.1126\/science.1250684"},{"key":"B4","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmet.2016.10.008"},{"key":"B5","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2016.11.011"},{"key":"B6","doi-asserted-by":"publisher","DOI":"10.1126\/science.1251086"},{"key":"B7","doi-asserted-by":"publisher","DOI":"10.1007\/s00109-020-01915-w"},{"key":"B8","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2017.12.031"},{"key":"B9","doi-asserted-by":"publisher","DOI":"10.1016\/j.chom.2017.12.010"},{"key":"B10","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2018.09.042"},{"key":"B11","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2016.01.019"},{"key":"B12","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2018.03.118"},{"key":"B13","doi-asserted-by":"publisher","DOI":"10.1016\/j.ebiom.2018.02.009"},{"key":"B14","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0153289"},{"key":"B15","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0162497"},{"key":"B16","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1600266"},{"key":"B17","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.98449.4"},{"key":"B18","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2019.12.015"},{"key":"B19","doi-asserted-by":"publisher","DOI":"10.1038\/s41590-023-01428-x"},{"key":"B20","doi-asserted-by":"publisher","DOI":"10.1038\/s41590-023-01739-z"},{"key":"B21","doi-asserted-by":"publisher","DOI":"10.1038\/s41577-020-0285-6"},{"key":"B22","doi-asserted-by":"publisher","DOI":"10.1038\/s41573-019-0025-4"},{"key":"B23","unstructured":"World Health Organization. WHO Bacterial Priority Pathogens List, 2024: bacterial pathogens of public health importance to guide research, development and strategies to prevent and control antimicrobial resistance. Geneva: World Health Organization; 2024. Licence: CC BY-NC-SA 3.0 IGO. https:\/\/www.who.int\/publications\/i\/item\/9789240093461"},{"key":"B24","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0811273106"},{"key":"B25","doi-asserted-by":"publisher","DOI":"10.1086\/656236"},{"key":"B26","doi-asserted-by":"publisher","DOI":"10.1038\/ng1976"},{"key":"B27","doi-asserted-by":"publisher","DOI":"10.1172\/JCI162581"},{"key":"B28","doi-asserted-by":"publisher","DOI":"10.1161\/ATVBAHA.114.303887"},{"key":"B29","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2020.10.011"},{"key":"B30","doi-asserted-by":"publisher","DOI":"10.1038\/sigtrans.2017.23"},{"key":"B31","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.90.24.11503"},{"key":"B32","doi-asserted-by":"publisher","DOI":"10.1128\/mcb.10.6.2448-2457.1990"},{"key":"B33","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1009220"},{"key":"B34","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.274.16.10689"},{"key":"B35","doi-asserted-by":"publisher","DOI":"10.1016\/j.cellimm.2009.10.007"},{"key":"B36","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.261570598"},{"key":"B37","doi-asserted-by":"publisher","DOI":"10.1038\/s41392-023-01608-z"},{"key":"B38","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.181.10.7316"},{"key":"B39","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1501612"},{"key":"B40","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2021.657261"},{"key":"B41","doi-asserted-by":"publisher","DOI":"10.1038\/s41385-022-00528-5"},{"key":"B42","doi-asserted-by":"publisher","DOI":"10.20900\/immunometab20210025"},{"key":"B43","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-023-43315-x"},{"key":"B44","doi-asserted-by":"publisher","DOI":"10.1038\/ni.1992"},{"key":"B45","doi-asserted-by":"crossref","unstructured":"Berry SB, et al. Disruption of aldehyde dehydrogenase 2 protects against bacterial infection [preprint]. https:\/\/doi.org\/10.1101\/2023.08.24.554661 Posted on bioRxiv October 18, 2025","DOI":"10.1101\/2023.08.24.554661"},{"key":"B46","doi-asserted-by":"publisher","DOI":"10.1186\/s13071-020-04331-8"},{"key":"B47","doi-asserted-by":"publisher","DOI":"10.1586\/eri.12.77"},{"key":"B48","doi-asserted-by":"publisher","DOI":"10.1093\/bmb\/ldw009"},{"key":"B49","doi-asserted-by":"crossref","unstructured":"O\u2019Farrell A, et al. Innate immune memory is stimulus specific [preprint]. https:\/\/doi.org\/10.1101\/2025.01.22.634275 Posted on bioRxiv January 24, 2025","DOI":"10.1101\/2025.01.22.634275"},{"key":"B50","doi-asserted-by":"publisher","DOI":"10.1038\/35092578"},{"key":"B51","doi-asserted-by":"publisher","DOI":"10.1038\/nature01182"},{"key":"B52","doi-asserted-by":"publisher","DOI":"10.1038\/gene.2013.48"},{"key":"B53","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2350-10-65"},{"key":"B54","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmet.2015.01.017"},{"key":"B55","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-023-40021-6"},{"key":"B56","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-019-13636-x"},{"key":"B57","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.2000532"},{"key":"B58","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2020.107634"},{"issue":"1","key":"B59","first-page":"56","article-title":"Interleukin-10 is upregulated in LPS tolerance","volume":"45","author":"Frankenberger","year":"1995","journal-title":"J Inflamm"},{"key":"B60","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2019.01109"},{"key":"B61","doi-asserted-by":"publisher","DOI":"10.1128\/CVI.00349-16"},{"key":"B62","doi-asserted-by":"publisher","DOI":"10.1016\/j.isci.2024.111284"},{"key":"B63","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2022.12.038"},{"key":"B64","doi-asserted-by":"publisher","DOI":"10.3390\/cells10050971"},{"key":"B65","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2018.03155"},{"key":"B66","doi-asserted-by":"publisher","DOI":"10.1093\/cvr\/cvz137"},{"key":"B67","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2014-04-568451"},{"key":"B68","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1001893"},{"key":"B69","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20102049"},{"key":"B70","doi-asserted-by":"publisher","DOI":"10.1038\/ni.1831"},{"key":"B71","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms7588"},{"key":"B72","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa2212616"},{"key":"B73","doi-asserted-by":"publisher","DOI":"10.1128\/cdli.11.1.161-167.2004"},{"key":"B74","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0182427"},{"key":"B75","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0224276"},{"key":"B76","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2018.00298"},{"key":"B77","doi-asserted-by":"publisher","DOI":"10.1016\/j.xcrm.2022.100817"},{"key":"B78","doi-asserted-by":"publisher","DOI":"10.1016\/j.xcrm.2024.101829"},{"key":"B79","doi-asserted-by":"publisher","DOI":"10.1038\/ni.3398"},{"key":"B80","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2021.663695"},{"key":"B81","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.4396"},{"key":"B82","doi-asserted-by":"publisher","DOI":"10.1186\/gb-2008-9-9-r137"},{"key":"B83","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-019-45839-z"},{"key":"B84","doi-asserted-by":"publisher","DOI":"10.1186\/s13059-014-0550-8"},{"key":"B85","doi-asserted-by":"publisher","DOI":"10.1038\/nbt.1630"},{"key":"B86","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-020-18035-1"}],"container-title":["JCI Insight"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/insight.jci.org\/articles\/view\/195866\/files\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,15]],"date-time":"2026-05-15T19:27:03Z","timestamp":1778873223000},"score":1,"resource":{"primary":{"URL":"https:\/\/insight.jci.org\/articles\/view\/195866"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,2,10]]},"references-count":86,"journal-issue":{"issue":"6","published-online":{"date-parts":[[2026,3,23]]},"published-print":{"date-parts":[[2026,3,23]]}},"alternative-id":["10.1172\/jci.insight.195866"],"URL":"https:\/\/doi.org\/10.1172\/jci.insight.195866","relation":{},"ISSN":["2379-3708"],"issn-type":[{"value":"2379-3708","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,2,10]]},"article-number":"e195866"}}