{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,7]],"date-time":"2026-09-07T18:09:14Z","timestamp":1788804554783,"version":"build-2803163510"},"reference-count":80,"publisher":"American Society for Clinical Investigation","issue":"17","license":[{"start":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T00:00:00Z","timestamp":1788825600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"NIH\/NIAID","award":["R01AI153829"],"award-info":[{"award-number":["R01AI153829"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,9,8]]},"abstract":"<jats:p>\n                    Despite widespread vaccination,\n                    <jats:italic>Bordetella pertussis<\/jats:italic>\n                    (\n                    <jats:italic>Bp<\/jats:italic>\n                    ) cases are resurging globally. Although CD4\n                    <jats:sup>+<\/jats:sup>\n                    T cells are known to be essential for sustained protection, the antigens they recognize are not fully characterized, hindering vaccine refinement. Using immunopeptidomics, bioinformatics, and functional T cell assays, we identified high-affinity epitopes from reference and clinical\n                    <jats:italic>Bp<\/jats:italic>\n                    strains presented on MHC-II I-A\n                    <jats:sup>b<\/jats:sup>\n                    . A subset of these epitopes stimulated systemic and mucosal CD4\n                    <jats:sup>+<\/jats:sup>\n                    T cells of mice immunized with heat-killed\n                    <jats:italic>Bp<\/jats:italic>\n                    , and peripheral blood T cells from humans vaccinated with the whole-cell pertussis vaccine. Mice immunized with a subunit vaccine comprising two recombinant proteins identified in our screen were subsequently challenged with\n                    <jats:italic>Bp<\/jats:italic>\n                    . Bacterial burden was nearly eliminated from the lower respiratory tract and significantly reduced in the upper respiratory tract. Th1\/Th17-polarized CD4\n                    <jats:sup>+<\/jats:sup>\n                    tissue-resident memory T cells (Trms) were induced in nasal and pulmonary tissues. Depleting memory CD4\n                    <jats:sup>+<\/jats:sup>\n                    T cells before challenge abolished protection, confirming that antigen-specific CD4\n                    <jats:sup>+<\/jats:sup>\n                    T cells are critical for clearing\n                    <jats:italic>Bp<\/jats:italic>\n                    from the respiratory tract. Our integrated antigen identification and T cell assay approach revealed previously untested\n                    <jats:italic>Bp<\/jats:italic>\n                    antigens that elicit protective CD4\n                    <jats:sup>+<\/jats:sup>\n                    T cell\u2013mediated immunity, suggesting that incorporating them into new vaccines may help curb the resurgence of pertussis.\n                  <\/jats:p>","DOI":"10.1172\/jci.insight.199234","type":"journal-article","created":{"date-parts":[[2026,9,7]],"date-time":"2026-09-07T18:01:06Z","timestamp":1788804066000},"source":"Crossref","is-referenced-by-count":0,"title":["Discovery of CD4+ T cell\u2013recognized B. pertussis antigens that reduce airway colonization"],"prefix":"10.1172","volume":"11","author":[{"given":"Mohamed M.","family":"Shamseldin","sequence":"first","affiliation":[{"name":"Department of Microbial Infection and Immunity and"},{"name":"Department of Microbiology, The Ohio State University, Columbus, 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