{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,24]],"date-time":"2026-10-24T21:13:37Z","timestamp":1792876417086,"version":"4.5.0"},"reference-count":73,"publisher":"American Society for Clinical Investigation","issue":"10","license":[{"start":{"date-parts":[[2026,5,22]],"date-time":"2026-05-22T00:00:00Z","timestamp":1779408000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000024","name":"Canadian Institutes of Health Research","doi-asserted-by":"crossref","award":["CIHR PJT-175066"],"award-info":[{"award-number":["CIHR PJT-175066"]}],"id":[{"id":"10.13039\/501100000024","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100000024","name":"Canadian Institutes of Health Research","doi-asserted-by":"crossref","award":["CIHR PJT-175066"],"award-info":[{"award-number":["CIHR PJT-175066"]}],"id":[{"id":"10.13039\/501100000024","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,5,22]]},"abstract":"<jats:p>\n                    Brain metastases (BrMs) occur in approximately 30% of cancer patients, causing nearly one-fifth of cancer deaths. While immune checkpoint inhibitors (ICIs) benefit some BrM patients, responses remain highly variable. This variability partly reflects distinct histopathological growth patterns that include minimally invasive (MI) and highly invasive (HI) brain BrMs. Here we show that MI BrMs exhibit robust immune infiltration, whereas HI lesions are immunosuppressed. However, histological differentiation between MI and HI can be challenging because of subjective margin assessment. Here, using highly multiplexed spatial proteomics on 119 tumor sections from 46 patients with BrMs, we identify CHI3L1 as a key mediator of the immunosuppressive microenvironment in HI BrMs. In preclinical models, genetic deletion of CHI3L1 converts immune-cold metastases into lymphocyte-rich, ICI-responsive lesions infiltrated by granzyme B\n                    <jats:sup>+<\/jats:sup>\n                    CD8\n                    <jats:sup>+<\/jats:sup>\n                    T cells. In BrM patients treated with ICI, immunohistochemical quantification of CHI3L1 expression was a stronger predictor of ICI response than traditional MI\/HI classification. Thus, CHI3L1 represents a promising biomarker and therapeutic target for BrMs.\n                  <\/jats:p>","DOI":"10.1172\/jci.insight.199498","type":"journal-article","created":{"date-parts":[[2026,5,21]],"date-time":"2026-05-21T18:00:52Z","timestamp":1779386452000},"source":"Crossref","is-referenced-by-count":1,"title":["Distinct immune landscapes characterize highly versus minimally invasive brain metastases"],"prefix":"10.1172","volume":"11","author":[{"given":"Sarah M.","family":"Maritan","sequence":"first","affiliation":[{"name":"Goodman Cancer Institute,"},{"name":"Department of Medicine, Division of Experimental Medicine,"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elham","family":"Karimi","sequence":"additional","affiliation":[{"name":"Goodman Cancer Institute,"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthew","family":"Dankner","sequence":"additional","affiliation":[{"name":"Goodman Cancer Institute,"},{"name":"Department of 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