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Pathogenic variants in the voltage-gated potassium channel KCNQ1 are the most frequently discovered genetic cause. Most LQTS-associated KCNQ1 variants cause loss of function secondary to impaired trafficking of the channel to the plasma membrane. There are currently no therapeutic approaches that address this underlying molecular defect. Using a high-throughput screening paradigm, we identified VU0494372, a small molecule that increases total and cell surface levels and trafficking efficiency of WT KCNQ1 as well as three LQTS-associated variants. Additionally, 16-hour treatment of cells with VU0494372 increased I\n                    <jats:sub>\n                      <jats:sub>Ks<\/jats:sub>\n                    <\/jats:sub>\n                    (KCNQ1-KCNE1 current) for WT KCNQ1 and the LQTS-associated variant V207M in cells coexpressing KCNE1. VU0494372 had no impact on KCNQ1 transcription, degradation, or thermal stability, and increased the rate of KCNQ1 reaching the cell surface. We identified a potential direct interaction site with KCNQ1 at or near the binding site of the KCNQ1 potentiator ML277. Together, these findings demonstrate that small molecules can increase the expression levels and cell surface trafficking efficiency of KCNQ1 and introduce a potential new pharmacological approach for treating LQTS.\n                  <\/jats:p>","DOI":"10.1172\/jci.insight.201297","type":"journal-article","created":{"date-parts":[[2026,1,8]],"date-time":"2026-01-08T17:00:37Z","timestamp":1767891637000},"source":"Crossref","is-referenced-by-count":2,"title":["High-throughput screening identifies a trafficking corrector for long QT syndrome\u2013associated KCNQ1 variants"],"prefix":"10.1172","volume":"11","author":[{"given":"Katherine R. 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