{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T20:33:36Z","timestamp":1792787616540,"version":"4.5.0"},"reference-count":31,"publisher":"American Society for Clinical Investigation","issue":"20","license":[{"start":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T00:00:00Z","timestamp":1792627200000},"content-version":"vor","delay-in-days":37,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Heart, Lung, and Blood Institute","award":["1R38HL143612,5T32HL160494-03,1K38HL180998-01"],"award-info":[{"award-number":["1R38HL143612,5T32HL160494-03,1K38HL180998-01"]}],"id":[{"id":"https:\/\/ror.org\/012pb6c26","id-type":"ROR","asserted-by":"crossref"}]},{"name":"CHEST Foundation","award":["n\/a"],"award-info":[{"award-number":["n\/a"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,10,22]]},"abstract":"<jats:p>\n                    Despite advances in treatment approaches for lung cancer, the morbidity and survival of patients with lung cancer with malignant pleural effusions (MPE) remain poor. This is in part due to gaps in understanding the role of immune cells in the pleural fluid microenvironment. We performed single-cell analysis with flow cytometry validation of CD45\n                    <jats:sup>+<\/jats:sup>\n                    cells in 8 MPE and 5 benign pleural fluid effusion (BPE) specimens to identify changes in the transcriptomic landscape of immune cells across disease states. We found upregulation of proinflammatory signaling pathways, including IFN and TNF signaling, in T cells, B cells, and macrophages in benign compared with malignant pleural effusions. Proinflammatory HLA-DR\n                    <jats:sup>+<\/jats:sup>\n                    macrophages were associated with good survival outcomes while protumorigenic HLA-DR\n                    <jats:sup>\u2013<\/jats:sup>\n                    macrophages with upregulation of angiogenesis, TGF-\u03b2, and fibronectin signaling were associated with poor survival outcomes in patients with MPE. We also validated these findings with macrophage cell surface expression markers using flow cytometry in 14 MPE and 7 BPE specimens. Finally, we performed multiplex cytokine analysis, which showed enrichment of the type 3 inflammatory cytokine, IL-17A, in MPE as a putative mechanism for macrophage reprogramming. These data provide a rich resource for interrogating the immune cell types and states present across the spectrum of pleural disease. They offer not only prognostic value for patient outcomes at the time of pleural fluid collection but also insights into novel immunotherapy targets.\n                  <\/jats:p>","DOI":"10.1172\/jci.insight.201691","type":"journal-article","created":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T16:01:20Z","timestamp":1789488080000},"source":"Crossref","is-referenced-by-count":0,"title":["Transcriptomic profiling of immune cells in malignant pleural effusions identifies macrophage reprogramming associated with survival"],"prefix":"10.1172","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4372-2324","authenticated-orcid":true,"given":"Aaditya","family":"Khatri","sequence":"first","affiliation":[{"name":"Department of Medicine, Division of Pulmonary, Allergy and Critical Care Medicine;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huimin","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Biostatistics and Bioinformatics;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhicheng","family":"Ji","sequence":"additional","affiliation":[{"name":"Department of Biostatistics and Bioinformatics;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Prekshaben","family":"Patel","sequence":"additional","affiliation":[{"name":"Department of Surgery;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7019-1912","authenticated-orcid":true,"given":"Smita K.","family":"Nair","sequence":"additional","affiliation":[{"name":"Department of Surgery;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9993-7407","authenticated-orcid":true,"given":"Javid P.","family":"Mohammed","sequence":"additional","affiliation":[{"name":"Department of Integrative Immunobiology;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2270-4821","authenticated-orcid":true,"given":"Beth H.","family":"Shaz","sequence":"additional","affiliation":[{"name":"Department of Pathology;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrew B.","family":"Nixon","sequence":"additional","affiliation":[{"name":"Department of Medicine, Division of Medical Oncology; 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