{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,15]],"date-time":"2026-05-15T20:07:50Z","timestamp":1778875670099,"version":"3.51.4"},"reference-count":28,"publisher":"American Society for Clinical Investigation","issue":"8","license":[{"start":{"date-parts":[[2026,4,22]],"date-time":"2026-04-22T00:00:00Z","timestamp":1776816000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000050","name":"National Heart Lung and Blood Institute","doi-asserted-by":"crossref","award":["1R01HL169238-01A1"],"award-info":[{"award-number":["1R01HL169238-01A1"]}],"id":[{"id":"10.13039\/100000050","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,4,22]]},"abstract":"<jats:p>\n                    Vascularized skins were 3D printed using single donor human fibroblasts, pericytes, keratinocytes, and endothelial cells (ECs), the latter either unmodified (WT-ECs) or deleted of MHC molecules (KO-ECs). Adult MISTRG6 immunodeficient mice neonatally inoculated with adult human hematopoietic stem cells (HSCs) received printed skin allogeneic to the HSCs and were boosted 3 weeks after grafting with human PBMCs autologous to the HSCs. HSC inoculation alone produced low levels of circulating human myeloid and lymphoid cells without affecting grafts; PBMC boosting dramatically increased circulating human CD4\n                    <jats:sup>+<\/jats:sup>\n                    T cells and boosted CD8\n                    <jats:sup>+<\/jats:sup>\n                    T cells only in mice with WT-EC grafts. These grafts became infiltrated by human macrophages, dendritic cells, CD4\n                    <jats:sup>+<\/jats:sup>\n                    and CD8\n                    <jats:sup>+<\/jats:sup>\n                    T cells and showed evidence of rejection. Shared T cell clones were present in skin and spleen. KO-EC grafts had minimal infiltration of graft or spleen without rejection, despite MHC molecule expression on other graft cell types.\n                  <\/jats:p>","DOI":"10.1172\/jci.insight.201946","type":"journal-article","created":{"date-parts":[[2026,4,21]],"date-time":"2026-04-21T18:00:45Z","timestamp":1776794445000},"source":"Crossref","is-referenced-by-count":0,"title":["Endothelial MHC expression is required to initiate T cell\u2013mediated rejection of 3D-printed skin grafts"],"prefix":"10.1172","volume":"11","author":[{"given":"Zuzana","family":"Tobiasova","sequence":"first","affiliation":[{"name":"Department of Immunobiology,"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Esen","family":"Sefik","sequence":"additional","affiliation":[{"name":"Department of Immunobiology,"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lingfeng","family":"Qin","sequence":"additional","affiliation":[{"name":"Department of Surgery,"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8142-6481","authenticated-orcid":true,"given":"Jennifer M.","family":"McNiff","sequence":"additional","affiliation":[{"name":"Department of Dermatology, and"},{"name":"Department of Pathology Yale School of Medicine, Yale University, New Haven, Connecticut, USA."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gwendolyn","family":"Davis","sequence":"additional","affiliation":[{"name":"Department of Immunobiology,"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4461-0778","authenticated-orcid":true,"given":"Richard A.","family":"Flavell","sequence":"additional","affiliation":[{"name":"Department of Immunobiology,"},{"name":"Howard Hughes Medical Institute, New Haven, Connecticut, USA."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"W. 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