{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,21]],"date-time":"2026-06-21T18:47:49Z","timestamp":1782067669453,"version":"3.54.5"},"reference-count":82,"publisher":"American Society for Clinical Investigation","issue":"12","license":[{"start":{"date-parts":[[2026,6,22]],"date-time":"2026-06-22T00:00:00Z","timestamp":1782086400000},"content-version":"vor","delay-in-days":55,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Bluesphere Bio","award":["no number"],"award-info":[{"award-number":["no number"]}]},{"DOI":"10.13039\/100007408","name":"University of Pittsburgh Medical Center","doi-asserted-by":"crossref","award":["no grant number"],"award-info":[{"award-number":["no grant number"]}],"id":[{"id":"10.13039\/100007408","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,6,22]]},"abstract":"<jats:p>\n                    Tumor-infiltrating CD8 cells recognize neoantigens created by tumor-specific mutations. Nonetheless, even after checkpoint inhibitor therapy, most patients\u2019 tumors progress. A deeper understanding of antitumor responses could facilitate development of better therapies. To enable such studies, we applied TCXpress, a high throughput platform that clones fully expressible TCRs from single cells into retroviral or lentiviral vectors without sequencing or gene synthesis, to study TCRs from CD8 cells infiltrating mouse MC38 tumors. We expressed cloned TCRs in reporter cells and interrogated TCR specificity by coculturing them with B6WT3 cells transduced with tandem minigenes encoding predicted neoantigens. We isolated TCRs reactive against epitopes from mutant\n                    <jats:italic>Rpl18<\/jats:italic>\n                    ,\n                    <jats:italic>Adpgk<\/jats:italic>\n                    ,\n                    <jats:italic>Psmd2<\/jats:italic>\n                    , and\n                    <jats:italic>Zc3h7b<\/jats:italic>\n                    along with self-reactive TCRs that recognized normal B6 and MC38 cells. Importantly, we successfully treated MC38-bearing mice with T cells transduced with anti-Rpl18 TCRs. These results establish a system that could be used to study many types of T cell responses and validate a therapeutic approach that could be tested in the clinic.\n                  <\/jats:p>","DOI":"10.1172\/jci.insight.203622","type":"journal-article","created":{"date-parts":[[2026,4,28]],"date-time":"2026-04-28T16:00:17Z","timestamp":1777392017000},"source":"Crossref","is-referenced-by-count":0,"title":["A platform for parallel TCR cloning and testing enables anti-neoantigen tumor immunotherapy"],"prefix":"10.1172","volume":"11","author":[{"given":"Alexander M.","family":"Rowe","sequence":"first","affiliation":[{"name":"Department of Immunology, University of Pittsburgh, Pittsburgh, Pennsylvania, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Smriti","family":"Chaurasia","sequence":"additional","affiliation":[{"name":"Department of Immunology, University of Pittsburgh, Pittsburgh, Pennsylvania, 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