{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T04:51:23Z","timestamp":1790052683474,"version":"4.0.1"},"reference-count":27,"publisher":"American Society for Clinical Investigation","issue":"8","license":[{"start":{"date-parts":[[2026,4,22]],"date-time":"2026-04-22T00:00:00Z","timestamp":1776816000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100010269","name":"Wellcome Trust","doi-asserted-by":"crossref","award":["224586\/Z\/21\/Z"],"award-info":[{"award-number":["224586\/Z\/21\/Z"]}],"id":[{"id":"10.13039\/100010269","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100011718","name":"Macular Society","doi-asserted-by":"crossref","award":["23-PhD-02"],"award-info":[{"award-number":["23-PhD-02"]}],"id":[{"id":"10.13039\/100011718","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100017645","name":"Moorfields Eye Charity","doi-asserted-by":"crossref","award":["GR001504,PhD-24-105,GR001475"],"award-info":[{"award-number":["GR001504,PhD-24-105,GR001475"]}],"id":[{"id":"10.13039\/501100017645","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,4,22]]},"abstract":"<jats:p>\n                    We generated a comparative spatial proteomic atlas of the human and mouse retina using a highly multiplexed immunohistochemistry technique called iterative bleaching extends multiplexity (IBEX). We refined the IBEX workflow by integrating an antibody dissociation option alongside chemical bleaching. This dual strategy enabled removal of the entire antibody complex, permitting the flexible use of antibodies from the same host species across iterative cycles. We coupled this workflow with super-resolution imaging via deconvolution and applied it to the retina of healthy humans and WT mice and the Crb1\n                    <jats:italic>\n                      <jats:sup>rd8<\/jats:sup>\n                    <\/jats:italic>\n                    mouse model. We successfully imaged over 25 protein markers on human and mouse tissue sections, generating spatial atlases of the major retinal cell populations. Cross-species protein expression was compared to scRNA-seq datasets to identify protein and transcript disparities. Super-resolution IBEX delineated the ultrastructural features of the outer limiting membrane (OLM), identifying CD44 as a core structural component tightly colocalized with a highly organized F-actin belt within M\u00fcller glial endfeet. Using the Crb1\n                    <jats:italic>\n                      <jats:sup>rd8<\/jats:sup>\n                    <\/jats:italic>\n                    mouse model, disruption of this complex was spatially associated with rosette formation and OLM structural failure. In summary, spatial proteomic atlases of the human and mouse retina were used to reveal insights into the arrangement of major retinal cell populations and OLM structure.\n                  <\/jats:p>","DOI":"10.1172\/jci.insight.204535","type":"journal-article","created":{"date-parts":[[2026,4,21]],"date-time":"2026-04-21T18:00:45Z","timestamp":1776794445000},"source":"Crossref","is-referenced-by-count":1,"title":["Spatial proteomic mapping of the human and mouse retina using IBEX"],"prefix":"10.1172","volume":"11","author":[{"given":"Yuxuan","family":"Meng","sequence":"first","affiliation":[{"name":"UCL Institute of Ophthalmology, London, United Kingdom."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jakub","family":"Kubiak","sequence":"additional","affiliation":[{"name":"UCL Institute of Ophthalmology, London, United 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