{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,4]],"date-time":"2026-11-04T03:27:11Z","timestamp":1793762831914,"version":"4.6.1"},"reference-count":63,"publisher":"American Society for Clinical Investigation","issue":"12","license":[{"start":{"date-parts":[[2026,6,22]],"date-time":"2026-06-22T00:00:00Z","timestamp":1782086400000},"content-version":"vor","delay-in-days":55,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"NIH","award":["NIH R37 AI045898"],"award-info":[{"award-number":["NIH R37 AI045898"]}]},{"name":"NIH","award":["U19 AI070235"],"award-info":[{"award-number":["U19 AI070235"]}]},{"name":"NIH","award":["R01 DK140127"],"award-info":[{"award-number":["R01 DK140127"]}]},{"name":"The Cincinnati Children\u2019s Hospital Medical","award":["The Cincinnati Children\u2019s Hospital Medical Research Foundation"],"award-info":[{"award-number":["The Cincinnati Children\u2019s Hospital Medical Research Foundation"]}]},{"name":"the Campaign Urging Research for Eosinophilic Disease","award":["CURED"],"award-info":[{"award-number":["CURED"]}]},{"name":"Sunshine Charitable Foundation","award":["Sunshine Charitable Foundation"],"award-info":[{"award-number":["Sunshine Charitable Foundation"]}]},{"name":"Denise and David Bunning","award":["Denise"],"award-info":[{"award-number":["Denise"]}]},{"name":"Denise and David Bunning","award":["David Bunning"],"award-info":[{"award-number":["David Bunning"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,6,22]]},"abstract":"<jats:p>\n                    Eosinophilic esophagitis (EoE) is a type 2 allergic disease characterized by esophageal inflammation and epithelial cell dysfunction. The acquired loss of the anti\u2013serine protease of kazal type 7 (anti-SPINK7) in the squamous epithelium of the esophagus has a causal role in EoE pathogenesis. However, there is a limited understanding of the factors that regulate its expression and responsiveness to inflammatory stimuli. Herein, we have identified the transcription factor, ovo like transcriptional repressor 1 (OVOL1), as an esophageal selective gene product that regulates SPINK7 promoter activity. Overexpression of\n                    <jats:italic>OVOL1<\/jats:italic>\n                    increased\n                    <jats:italic>SPINK7<\/jats:italic>\n                    expression, whereas its depletion decreased\n                    <jats:italic>SPINK7<\/jats:italic>\n                    expression, impaired epithelial barrier, and increased production of the proatopy cytokine thymic stromal lymphopoietin (TSLP). Stimulation with IL-13 abrogated the nuclear translocation of OVOL1 and promoted enhanced degradation of OVOL1 protein. This effect of IL-13 was dependent on the esophageal specific cysteine protease calpain-14 at least in part. Analysis of human esophageal biopsies demonstrated that the expression of esophageal OVOL1 correlated with\n                    <jats:italic>SPINK7<\/jats:italic>\n                    transcript expression and was lost as a function of EoE disease activity. In summary, our study identifies key regulatory mechanisms in EoE pathogenesis, demonstrating that OVOL1 promotes SPINK7 transcription, whereas IL-13 suppresses this pathway in EoE.\n                  <\/jats:p>","DOI":"10.1172\/jci.insight.204687","type":"journal-article","created":{"date-parts":[[2026,4,28]],"date-time":"2026-04-28T16:01:31Z","timestamp":1777392091000},"source":"Crossref","is-referenced-by-count":4,"title":["IL-13 and calpain-14 suppress the expression of SPINK7 by regulating OVOL1 in eosinophilic esophagitis"],"prefix":"10.1172","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4969-1263","authenticated-orcid":true,"given":"Nurit P.","family":"Azouz","sequence":"first","affiliation":[{"name":"Division of Allergy and Immunology, Cincinnati Children\u2019s Hospital Medical Center, Department of Pediatrics, University of Cincinnati College of Medicine, Cincinnati, Ohio, USA."},{"name":"Department of Pediatrics, University of Cincinnati College of 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