{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T18:40:49Z","timestamp":1792608049416,"version":"4.5.0"},"reference-count":69,"publisher":"American Society for Clinical Investigation","issue":"20","license":[{"start":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T00:00:00Z","timestamp":1792627200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"NIH Grant","award":["DK137172,# CA286036"],"award-info":[{"award-number":["DK137172,# CA286036"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,10,22]]},"abstract":"<jats:p>\n                    Septins are RAS-like GTP-binding proteins involved in cytoskeletal organization and cellular division. We previously described 2 pediatric patients harboring\n                    <jats:italic>SEPT6<\/jats:italic>\n                    germline mutations (GM1: SEPT6 c.1282T&gt;C and GM2: SEPT6 c.1282T&gt;A) presenting with congenital neutropenia associated with the development of myelodysplastic syndrome. In one patient, an additional somatic mutation (SM: SEPT6 c.43C&gt;T) was detected at a low allelic fraction in the BM. Here, we describe the consequences of these mutations for hematopoiesis. Transgenic expression or gene editing of GM1 or GM2 in human CD34\n                    <jats:sup>+<\/jats:sup>\n                    cells led to enlarged, multinucleated cells associated with accelerated exhaustion and premature senescence of hematopoietic stem and progenitor cells (HSPCs). Senescence defects were associated with the upregulation of mTOR phosphorylation, and pharmacologic inhibition of the mTOR pathway resulted in rescue of senescence. In parallel, HSPCs exhibited marked mitochondrial abnormalities, including increased expression of OPA-1, a key regulator of mitochondrial fusion, and enhanced oxygen consumption during mitochondrial respiration. Introduction of the SM in\n                    <jats:italic>cis<\/jats:italic>\n                    with GM1 mitigated all observed defects. Collectively, our findings demonstrate a gain-of-function mechanism underlying marrow failure in patients carrying germline\n                    <jats:italic>SEPT6<\/jats:italic>\n                    mutations and suggest that acquisition of a secondary somatic SEPT6 variant may confer a compensatory effect within hematopoietic cells.\n                  <\/jats:p>","DOI":"10.1172\/jci.insight.209595","type":"journal-article","created":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T18:00:34Z","timestamp":1792605634000},"source":"Crossref","is-referenced-by-count":0,"title":["Patient-derived neomorphic SEPTIN6 mutations induce hematopoietic stem cell senescence underlying congenital neutropenia and myelodysplasia"],"prefix":"10.1172","volume":"11","author":[{"given":"Safa F.","family":"Mohamad","sequence":"first","affiliation":[{"name":"Division of Hematology\/Oncology, Boston Children\u2019s Hospital, Boston, Massachusetts, USA."},{"name":"Department of Pediatrics, Harvard Medical 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