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A single immunization with an attenuated strain of CHIKV (\u03945nsP3), a homologous prime-boost immunization with a DNA-launched RNA replicon encoding CHIKV envelope proteins (DREP-E), and a DREP-E prime followed by a recombinant modified vaccinia virus Ankara encoding CHIKV capsid and envelope (MVA-CE) boost all induced protection against WT CHIKV infection. The attenuated \u03945nsP3 virus proved to be safe and did not show any clinical signs typically associated with WT CHIKV infections such as fever, skin rash, lymphopenia, or joint swelling. These vaccines are based on an East\/Central\/South African strain of Indian Ocean lineage, but they also generated neutralizing antibodies against an isolate of the Asian genotype that now is rapidly spreading across the Americas. These results form the basis for clinical development of an efficacious CHIKV vaccine that generates both humoral and cellular immunity with long-term immunological memory.<\/jats:p>","DOI":"10.1172\/jci.insight.83527","type":"journal-article","created":{"date-parts":[[2017,3,22]],"date-time":"2017-03-22T11:01:42Z","timestamp":1490180502000},"source":"Crossref","is-referenced-by-count":96,"title":["Attenuated and vectored vaccines protect nonhuman primates against Chikungunya virus"],"prefix":"10.1172","volume":"2","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1825-1054","authenticated-orcid":true,"given":"Pierre","family":"Roques","sequence":"first","affiliation":[{"name":"Universit\u00e9 Paris Sud, UMR 1184, Orsay, France."},{"name":"CEA, DSV\/iMETI, Division of Immuno-Virology, IDMIT center,"},{"name":"Inserm, U1184, Center for immunology of viral infections and autoimmune diseases, Fontenay aux Roses, 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