{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T07:40:43Z","timestamp":1791272443384,"version":"4.1.0"},"reference-count":46,"publisher":"American Society for Clinical Investigation","issue":"15","funder":[{"name":"NIH","award":["DMID-NIAID U01 AI77887"],"award-info":[{"award-number":["DMID-NIAID U01 AI77887"]}]},{"name":"NIH","award":["R01 27530"],"award-info":[{"award-number":["R01 27530"]}]},{"name":"NIH","award":["RO1 27530"],"award-info":[{"award-number":["RO1 27530"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2016,9,22]]},"abstract":"<jats:p>\n                    We created and tested multi-epitope DNA or protein vaccines with TLR4 ligand emulsion adjuvant (gluco glucopyranosyl lipid adjuvant in a stable emulsion [GLA-SE]) for their ability to protect against\n                    <jats:italic>Toxoplasma gondii<\/jats:italic>\n                    in HLA transgenic mice. Our constructs each included 5 of our best down-selected CD8\n                    <jats:sup>+<\/jats:sup>\n                    T cell\u2013eliciting epitopes, a universal CD4\n                    <jats:sup>+<\/jats:sup>\n                    helper T lymphocyte epitope (PADRE), and a secretory signal,  all arranged for optimal MHC-I presentation. Their capacity to elicit immune and protective responses was studied using immunization of HLA-A*11:01 transgenic mice. These multi-epitope vaccines increased memory CD8\n                    <jats:sup>+<\/jats:sup>\n                    T cells that produced IFN-\u03b3 and protected mice against parasite burden when challenged with\n                    <jats:italic>T<\/jats:italic>\n                    .\n                    <jats:italic>gondii<\/jats:italic>\n                    . Endocytosis of emulsion-trapped protein and cross presentation of the antigens must account for the immunogenicity of our adjuvanted protein. Thus, our work creates an adjuvanted platform assembly of peptides resulting in cross presentation of CD8\n                    <jats:sup>+<\/jats:sup>\n                    T cell\u2013eliciting epitopes in a vaccine that prevents toxoplasmosis.\n                  <\/jats:p>","DOI":"10.1172\/jci.insight.85955","type":"journal-article","created":{"date-parts":[[2016,9,21]],"date-time":"2016-09-21T11:01:06Z","timestamp":1474455666000},"source":"Crossref","is-referenced-by-count":43,"title":["Adjuvanted multi-epitope vaccines protect HLA-A*11:01 transgenic mice against Toxoplasma gondii"],"prefix":"10.1172","volume":"1","author":[{"given":"Kamal","family":"El Bissati","sequence":"first","affiliation":[{"name":"Department of Opthalmology and Visual Science; and Department of Pediatrics, Infectious Diseases Division (RM), The University of Chicago, Chicago, Illinois, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aziz 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