{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T06:48:57Z","timestamp":1792651737447,"version":"4.5.0"},"reference-count":57,"publisher":"American Society for Clinical Investigation","issue":"5","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2016,4,21]]},"abstract":"<jats:p>\n                    Posttransplantation cyclophosphamide (PTCy) effectively prevents graft-versus-host disease (GVHD), but its immunologic impact is poorly understood. We assessed lymphocyte reconstitution via flow cytometry (\n                    <jats:italic>n<\/jats:italic>\n                    = 74) and antigen receptor sequencing (\n                    <jats:italic>n<\/jats:italic>\n                    = 35) in recipients of myeloablative, HLA-matched allogeneic BM transplantation using PTCy. Recovering T cells were primarily phenotypically effector memory with lower T cell receptor \u03b2 (\n                    <jats:italic>TRB<\/jats:italic>\n                    ) repertoire diversity than input donor repertoires. Recovering B cells were predominantly naive with immunoglobulin heavy chain locus (\n                    <jats:italic>IGH<\/jats:italic>\n                    ) repertoire diversity similar to donors. Numerical T cell reconstitution and\n                    <jats:italic>TRB<\/jats:italic>\n                    diversity were strongly associated with recipient cytomegalovirus seropositivity. Global similarity between input donor and recipient posttransplant repertoires was uniformly low at 1\u20132 months after transplant but increased over the balance of the first posttransplant year. Blood\n                    <jats:italic>TRB<\/jats:italic>\n                    repertoires at \u22653 months after transplant were often dominated by clones present in the donor blood\/marrow memory CD8\n                    <jats:sup>+<\/jats:sup>\n                    compartment. Limited overlap was observed between the\n                    <jats:italic>TRB<\/jats:italic>\n                    repertoires of T cells infiltrating the skin or gastrointestinal tract versus the blood. Although public\n                    <jats:italic>TRB<\/jats:italic>\n                    sequences associated with herpesvirus- or alloantigen-specific CD8\n                    <jats:sup>+<\/jats:sup>\n                    T cells were detected in some patients, posttransplant\n                    <jats:italic>TRB<\/jats:italic>\n                    and\n                    <jats:italic>IGH<\/jats:italic>\n                    repertoires were unique to each individual. These data define the immune dynamics occurring after PTCy and establish a benchmark against which immune recovery after other transplantation approaches can be compared.\n                  <\/jats:p>","DOI":"10.1172\/jci.insight.86252","type":"journal-article","created":{"date-parts":[[2016,4,21]],"date-time":"2016-04-21T09:10:49Z","timestamp":1461229849000},"source":"Crossref","is-referenced-by-count":143,"title":["Origin and evolution of the T cell repertoire after posttransplantation cyclophosphamide"],"prefix":"10.1172","volume":"1","author":[{"given":"Christopher G.","family":"Kanakry","sequence":"first","affiliation":[{"name":"Sidney Kimmel Comprehensive Cancer Center, The Johns Hopkins University School of Medicine, Baltimore, Maryland, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David G.","family":"Coffey","sequence":"additional","affiliation":[{"name":"Clinical Research Division, Fred Hutchinson Cancer Research Center (FHCRC), Seattle, Washington, 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